ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease

Abstract Background Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the development of coronary artery disease (CAD). ABCA1 and ABCG1 transporters regulate cell cholesterol content and reverse cholesterol transport. We aimed to determine whether DNA methylatio...

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Autores principales:	Valentina V. Miroshnikova, Alexandra A. Panteleeva, Irina A. Pobozheva, Natalia D. Razgildina, Ekaterina A. Polyakova, Anton V. Markov, Olga D. Belyaeva, Olga A. Berkovich, Elena I. Baranova, Maria S. Nazarenko, Valery P. Puzyrev, Sofya N. Pchelina
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Coronary artery disease ABCA1 and ABCG1 transporters Epicardial adipose tissue DNA methylation Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/82a825775b1641eea31803dca559b692
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spelling	oai:doaj.org-article:82a825775b1641eea31803dca559b6922021-11-28T12:05:18ZABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease10.1186/s12872-021-02379-71471-2261https://doaj.org/article/82a825775b1641eea31803dca559b6922021-11-01T00:00:00Zhttps://doi.org/10.1186/s12872-021-02379-7https://doaj.org/toc/1471-2261Abstract Background Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the development of coronary artery disease (CAD). ABCA1 and ABCG1 transporters regulate cell cholesterol content and reverse cholesterol transport. We aimed to determine whether DNA methylation and mRNA levels of the ABCA1 and ABCG1 genes in EAT and subcutaneous adipose tissue (SAT) were associated with CAD. Methods Paired EAT and SAT samples were collected from 82 patients undergoing elective cardiac surgery either for coronary artery bypass grafting (CAD group, N = 66) or valve surgery (NCAD group, N = 16). ABCA1 and ABCG1 mRNA levels in EAT and SAT samples were analyzed using real time polymerase chain reaction, ABCA1 protein levels in EAT samples were assessed by western blotting. ABCA1 and ABCG1 DNA methylation analysis was performed in 24 samples from the CAD group and 9 samples from the NCAD group via pyrosequencing. Results DNA methylation levels in the ABCA1 promoter and ABCG1 cg27243685 and cg06500161 CpG sites were higher in EAT samples from patients with CAD compared with NCAD (21.92% vs 10.81%, p = 0.003; 71.51% vs 68.42%, p = 0.024; 46.11% vs 37.79%, p = 0.016, respectively). In patients with CAD, ABCA1 and ABCG1 DNA methylation levels were higher in EAT than in SAT samples (p < 0.05). ABCA1 mRNA levels in EAT samples were reduced in the subgroup of patients with CAD and concomitant carotid artery disease or peripheral artery disease compared with the NCAD group (p = 0.024). ABCA1 protein levels in EAT samples tended to be lower in CAD patients than in the NCAD group (p = 0.053). DNA methylation levels at the ABCG1 cg27243685 site positively correlated with plasma triglyceride concentration (r = 0.510, p = 0.008), body mass index (r = 0.556, p = 0.013) and waist-to-hip ratio (r = 0.504, p = 0.012) in SAT samples. Conclusion CAD is associated with ABCA1 and ABCG1 DNA hypermethylation in EAT. CAD with concomitant carotid artery disease or peripheral artery disease is accompanied by decreased ABCA1 gene expression in EAT. DNA methylation levels at the ABCG1 cg27243685 locus in SAT are associated with hypertriglyceridemia and obesity.Valentina V. MiroshnikovaAlexandra A. PanteleevaIrina A. PobozhevaNatalia D. RazgildinaEkaterina A. PolyakovaAnton V. MarkovOlga D. BelyaevaOlga A. BerkovichElena I. BaranovaMaria S. NazarenkoValery P. PuzyrevSofya N. PchelinaBMCarticleCoronary artery diseaseABCA1 and ABCG1 transportersEpicardial adipose tissueDNA methylationDiseases of the circulatory (Cardiovascular) systemRC666-701ENBMC Cardiovascular Disorders, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Coronary artery disease ABCA1 and ABCG1 transporters Epicardial adipose tissue DNA methylation Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	Coronary artery disease ABCA1 and ABCG1 transporters Epicardial adipose tissue DNA methylation Diseases of the circulatory (Cardiovascular) system RC666-701 Valentina V. Miroshnikova Alexandra A. Panteleeva Irina A. Pobozheva Natalia D. Razgildina Ekaterina A. Polyakova Anton V. Markov Olga D. Belyaeva Olga A. Berkovich Elena I. Baranova Maria S. Nazarenko Valery P. Puzyrev Sofya N. Pchelina ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease
description	Abstract Background Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the development of coronary artery disease (CAD). ABCA1 and ABCG1 transporters regulate cell cholesterol content and reverse cholesterol transport. We aimed to determine whether DNA methylation and mRNA levels of the ABCA1 and ABCG1 genes in EAT and subcutaneous adipose tissue (SAT) were associated with CAD. Methods Paired EAT and SAT samples were collected from 82 patients undergoing elective cardiac surgery either for coronary artery bypass grafting (CAD group, N = 66) or valve surgery (NCAD group, N = 16). ABCA1 and ABCG1 mRNA levels in EAT and SAT samples were analyzed using real time polymerase chain reaction, ABCA1 protein levels in EAT samples were assessed by western blotting. ABCA1 and ABCG1 DNA methylation analysis was performed in 24 samples from the CAD group and 9 samples from the NCAD group via pyrosequencing. Results DNA methylation levels in the ABCA1 promoter and ABCG1 cg27243685 and cg06500161 CpG sites were higher in EAT samples from patients with CAD compared with NCAD (21.92% vs 10.81%, p = 0.003; 71.51% vs 68.42%, p = 0.024; 46.11% vs 37.79%, p = 0.016, respectively). In patients with CAD, ABCA1 and ABCG1 DNA methylation levels were higher in EAT than in SAT samples (p < 0.05). ABCA1 mRNA levels in EAT samples were reduced in the subgroup of patients with CAD and concomitant carotid artery disease or peripheral artery disease compared with the NCAD group (p = 0.024). ABCA1 protein levels in EAT samples tended to be lower in CAD patients than in the NCAD group (p = 0.053). DNA methylation levels at the ABCG1 cg27243685 site positively correlated with plasma triglyceride concentration (r = 0.510, p = 0.008), body mass index (r = 0.556, p = 0.013) and waist-to-hip ratio (r = 0.504, p = 0.012) in SAT samples. Conclusion CAD is associated with ABCA1 and ABCG1 DNA hypermethylation in EAT. CAD with concomitant carotid artery disease or peripheral artery disease is accompanied by decreased ABCA1 gene expression in EAT. DNA methylation levels at the ABCG1 cg27243685 locus in SAT are associated with hypertriglyceridemia and obesity.
format	article
author	Valentina V. Miroshnikova Alexandra A. Panteleeva Irina A. Pobozheva Natalia D. Razgildina Ekaterina A. Polyakova Anton V. Markov Olga D. Belyaeva Olga A. Berkovich Elena I. Baranova Maria S. Nazarenko Valery P. Puzyrev Sofya N. Pchelina
author_facet	Valentina V. Miroshnikova Alexandra A. Panteleeva Irina A. Pobozheva Natalia D. Razgildina Ekaterina A. Polyakova Anton V. Markov Olga D. Belyaeva Olga A. Berkovich Elena I. Baranova Maria S. Nazarenko Valery P. Puzyrev Sofya N. Pchelina
author_sort	Valentina V. Miroshnikova
title	ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease
title_short	ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease
title_full	ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease
title_fullStr	ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease
title_full_unstemmed	ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease
title_sort	abca1 and abcg1 dna methylation in epicardial adipose tissue of patients with coronary artery disease
publisher	BMC
publishDate	2021
url	https://doaj.org/article/82a825775b1641eea31803dca559b692
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ABCA1 and ABCG1 DNA methylation in epicardial adipose tissue of patients with coronary artery disease

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