Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.

<h4>Background</h4>Cell mediated immunity, including efficient CTL response, is required to prevent HIV-1 from cell-to-cell transmission. In previous investigations, we have shown that B1 peptide derived by Fourier transformation of HIV-1 primary structures and sharing no sequence homolo...

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Autores principales:	Ammar Achour, Jean-Michel Biquard, Velibor Krsmanovic, Jean-Pierre M'bika, Damien Ficheux, Marianna Sikorska, Alain J Cozzone
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82aaafaf9e67463e915190b29ebc5b01
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spelling	oai:doaj.org-article:82aaafaf9e67463e915190b29ebc5b012021-11-25T06:13:48ZInduction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.1932-620310.1371/journal.pone.0001214https://doaj.org/article/82aaafaf9e67463e915190b29ebc5b012007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0001214https://doaj.org/toc/1932-6203<h4>Background</h4>Cell mediated immunity, including efficient CTL response, is required to prevent HIV-1 from cell-to-cell transmission. In previous investigations, we have shown that B1 peptide derived by Fourier transformation of HIV-1 primary structures and sharing no sequence homology with the parent proteins was able to generate antiserum which recognizes envelope and Tat proteins. Here we have investigated cellular immune response towards a novel non-homologous peptide, referred to as cA1 peptide.<h4>Methodology/principal findings</h4>The 20 amino acid sequence of cA1 peptide was predicted using the notion of peptide hydropathic properties; the peptide is encoded by the complementary anti-sense DNA strand to the sense strand of previously described non-homologous A1 peptide. In this report we demonstrate that the cA1 peptide can be a target for major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes in HIV-1-infected or envelope-immunized individuals. The cA1 peptide is recognized in association with different MHC class I allotypes and could prime in vitro CTLs, derived from gp160-immunized individuals capable to recognize virus variants.<h4>Conclusions/significance</h4>For the first time a theoretically designed immunogen involved in broad-based cell-immune memory activation is described. Our findings may thus contribute to the advance in vaccine research by describing a novel strategy to develop a synthetic AIDS vaccine.Ammar AchourJean-Michel BiquardVelibor KrsmanovicJean-Pierre M'bikaDamien FicheuxMarianna SikorskaAlain J CozzonePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 2, Iss 11, p e1214 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ammar Achour Jean-Michel Biquard Velibor Krsmanovic Jean-Pierre M'bika Damien Ficheux Marianna Sikorska Alain J Cozzone Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
description	<h4>Background</h4>Cell mediated immunity, including efficient CTL response, is required to prevent HIV-1 from cell-to-cell transmission. In previous investigations, we have shown that B1 peptide derived by Fourier transformation of HIV-1 primary structures and sharing no sequence homology with the parent proteins was able to generate antiserum which recognizes envelope and Tat proteins. Here we have investigated cellular immune response towards a novel non-homologous peptide, referred to as cA1 peptide.<h4>Methodology/principal findings</h4>The 20 amino acid sequence of cA1 peptide was predicted using the notion of peptide hydropathic properties; the peptide is encoded by the complementary anti-sense DNA strand to the sense strand of previously described non-homologous A1 peptide. In this report we demonstrate that the cA1 peptide can be a target for major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes in HIV-1-infected or envelope-immunized individuals. The cA1 peptide is recognized in association with different MHC class I allotypes and could prime in vitro CTLs, derived from gp160-immunized individuals capable to recognize virus variants.<h4>Conclusions/significance</h4>For the first time a theoretically designed immunogen involved in broad-based cell-immune memory activation is described. Our findings may thus contribute to the advance in vaccine research by describing a novel strategy to develop a synthetic AIDS vaccine.
format	article
author	Ammar Achour Jean-Michel Biquard Velibor Krsmanovic Jean-Pierre M'bika Damien Ficheux Marianna Sikorska Alain J Cozzone
author_facet	Ammar Achour Jean-Michel Biquard Velibor Krsmanovic Jean-Pierre M'bika Damien Ficheux Marianna Sikorska Alain J Cozzone
author_sort	Ammar Achour
title	Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
title_short	Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
title_full	Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
title_fullStr	Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
title_full_unstemmed	Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
title_sort	induction of human immunodeficiency virus (hiv-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/82aaafaf9e67463e915190b29ebc5b01
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Induction of human immunodeficiency virus (HIV-1) envelope specific cell-mediated immunity by a non-homologous synthetic peptide.

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