Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage

Abstract Subarachnoid haemorrhage (SAH) is a type of hemorrhagic stroke that is associated with high morbidity and mortality. New effective treatments are needed to improve outcomes. The pathophysiology of SAH is complex and includes early brain injury and delayed cerebral ischemia, both of which ar...

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Autores principales:	Michael K. Tso, Paul Turgeon, Bert Bosche, Charles K. Lee, Tian Nie, Josephine D’Abbondanza, Jinglu Ai, Philip A. Marsden, R. Loch Macdonald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82ab040c092e4160870f46b7e2769ae7
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spelling	oai:doaj.org-article:82ab040c092e4160870f46b7e2769ae72021-12-02T14:15:53ZGene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage10.1038/s41598-021-87301-z2045-2322https://doaj.org/article/82ab040c092e4160870f46b7e2769ae72021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87301-zhttps://doaj.org/toc/2045-2322Abstract Subarachnoid haemorrhage (SAH) is a type of hemorrhagic stroke that is associated with high morbidity and mortality. New effective treatments are needed to improve outcomes. The pathophysiology of SAH is complex and includes early brain injury and delayed cerebral ischemia, both of which are characterized by blood–brain barrier (BBB) impairment. We isolated brain endothelial cells (BECs) from mice subjected to SAH by injection of blood into the prechiasmatic cistern. We used gene expression profiling to identify 707 unique genes (2.8% of transcripts, 403 upregulated, 304 downregulated, 24,865 interrogated probe sets) that were significantly differentially expressed in mouse BECs after SAH. The pathway involving prostaglandin synthesis and regulation was significantly upregulated after SAH, including increased expression of the Ptgs2 gene and its corresponding COX-2 protein. Celecoxib, a selective COX-2 inhibitor, limited upregulation of Ptgs2 in BECs. In this study, we have defined the gene expression profiling of BECs after experimental SAH and provide further insight into BBB pathophysiology, which may be relevant to other neurological diseases such as traumatic brain injury, brain tumours, ischaemic stroke, multiple sclerosis, and neurodegenerative disorders.Michael K. TsoPaul TurgeonBert BoscheCharles K. LeeTian NieJosephine D’AbbondanzaJinglu AiPhilip A. MarsdenR. Loch MacdonaldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael K. Tso Paul Turgeon Bert Bosche Charles K. Lee Tian Nie Josephine D’Abbondanza Jinglu Ai Philip A. Marsden R. Loch Macdonald Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
description	Abstract Subarachnoid haemorrhage (SAH) is a type of hemorrhagic stroke that is associated with high morbidity and mortality. New effective treatments are needed to improve outcomes. The pathophysiology of SAH is complex and includes early brain injury and delayed cerebral ischemia, both of which are characterized by blood–brain barrier (BBB) impairment. We isolated brain endothelial cells (BECs) from mice subjected to SAH by injection of blood into the prechiasmatic cistern. We used gene expression profiling to identify 707 unique genes (2.8% of transcripts, 403 upregulated, 304 downregulated, 24,865 interrogated probe sets) that were significantly differentially expressed in mouse BECs after SAH. The pathway involving prostaglandin synthesis and regulation was significantly upregulated after SAH, including increased expression of the Ptgs2 gene and its corresponding COX-2 protein. Celecoxib, a selective COX-2 inhibitor, limited upregulation of Ptgs2 in BECs. In this study, we have defined the gene expression profiling of BECs after experimental SAH and provide further insight into BBB pathophysiology, which may be relevant to other neurological diseases such as traumatic brain injury, brain tumours, ischaemic stroke, multiple sclerosis, and neurodegenerative disorders.
format	article
author	Michael K. Tso Paul Turgeon Bert Bosche Charles K. Lee Tian Nie Josephine D’Abbondanza Jinglu Ai Philip A. Marsden R. Loch Macdonald
author_facet	Michael K. Tso Paul Turgeon Bert Bosche Charles K. Lee Tian Nie Josephine D’Abbondanza Jinglu Ai Philip A. Marsden R. Loch Macdonald
author_sort	Michael K. Tso
title	Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
title_short	Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
title_full	Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
title_fullStr	Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
title_full_unstemmed	Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
title_sort	gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/82ab040c092e4160870f46b7e2769ae7
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Gene expression profiling of brain endothelial cells after experimental subarachnoid haemorrhage

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