Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum

With an aim to develop a highly attenuated and strongly immunogenic distinguishable vaccine candidate, a waaJ (a gene involved in the synthesis of lipopolysaccharide) and spiC (a virulence gene) double deletion Korean epidemic strain of S. enterica ser. Gallinarum (SG005) was constructed. Our result...

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Autores principales:	Jun-Feng Zhang, Ke Shang, Bai Wei, Yea-Jin Lee, Jong-Yeol Park, Hyung-Kwan Jang, Se-Yeoun Cha, Min Kang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	DIVA high attenuation waaJ S. enterica ser. Gallinarum double deletion spiC Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/82ab8223aec842ec8059bdf0def0bc3d
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spelling	oai:doaj.org-article:82ab8223aec842ec8059bdf0def0bc3d2021-11-16T06:34:16ZEvaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum2297-176910.3389/fvets.2021.756123https://doaj.org/article/82ab8223aec842ec8059bdf0def0bc3d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.756123/fullhttps://doaj.org/toc/2297-1769With an aim to develop a highly attenuated and strongly immunogenic distinguishable vaccine candidate, a waaJ (a gene involved in the synthesis of lipopolysaccharide) and spiC (a virulence gene) double deletion Korean epidemic strain of S. enterica ser. Gallinarum (SG005) was constructed. Our results showed that the growth and biochemical characteristics were not altered by this double deletion. The double deletion strain contained dual markers. One was a bacteriological marker (rough phenotype) and the other was a serological marker helping distinguish infected chickens from vaccinated chickens. The double deletion strain showed good genetic stability and reduced resistance to environmental stresses in vitro; furthermore, it was extremely safe and highly avirulent in broilers. Single intramuscular or oral immunization of 7-day-old broilers with the double deletion strain could stimulate the body to produce antibody levels similar to the conventional vaccine strain SG9R. In addition, against a lethal wild-type challenge, it conferred effective protection that was comparable to that seen in the group vaccinated with SG9R. In conclusion, this double deletion strain may be an effective vaccine candidate for controlling S. enterica ser. Gallinarum infection in broilers.Jun-Feng ZhangKe ShangBai WeiYea-Jin LeeJong-Yeol ParkHyung-Kwan JangSe-Yeoun ChaMin KangFrontiers Media S.A.articleDIVAhigh attenuationwaaJS. enterica ser. Gallinarumdouble deletionspiCVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DIVA high attenuation waaJ S. enterica ser. Gallinarum double deletion spiC Veterinary medicine SF600-1100
spellingShingle	DIVA high attenuation waaJ S. enterica ser. Gallinarum double deletion spiC Veterinary medicine SF600-1100 Jun-Feng Zhang Ke Shang Bai Wei Yea-Jin Lee Jong-Yeol Park Hyung-Kwan Jang Se-Yeoun Cha Min Kang Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
description	With an aim to develop a highly attenuated and strongly immunogenic distinguishable vaccine candidate, a waaJ (a gene involved in the synthesis of lipopolysaccharide) and spiC (a virulence gene) double deletion Korean epidemic strain of S. enterica ser. Gallinarum (SG005) was constructed. Our results showed that the growth and biochemical characteristics were not altered by this double deletion. The double deletion strain contained dual markers. One was a bacteriological marker (rough phenotype) and the other was a serological marker helping distinguish infected chickens from vaccinated chickens. The double deletion strain showed good genetic stability and reduced resistance to environmental stresses in vitro; furthermore, it was extremely safe and highly avirulent in broilers. Single intramuscular or oral immunization of 7-day-old broilers with the double deletion strain could stimulate the body to produce antibody levels similar to the conventional vaccine strain SG9R. In addition, against a lethal wild-type challenge, it conferred effective protection that was comparable to that seen in the group vaccinated with SG9R. In conclusion, this double deletion strain may be an effective vaccine candidate for controlling S. enterica ser. Gallinarum infection in broilers.
format	article
author	Jun-Feng Zhang Ke Shang Bai Wei Yea-Jin Lee Jong-Yeol Park Hyung-Kwan Jang Se-Yeoun Cha Min Kang
author_facet	Jun-Feng Zhang Ke Shang Bai Wei Yea-Jin Lee Jong-Yeol Park Hyung-Kwan Jang Se-Yeoun Cha Min Kang
author_sort	Jun-Feng Zhang
title	Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
title_short	Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
title_full	Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
title_fullStr	Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
title_full_unstemmed	Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum
title_sort	evaluation of safety and protective efficacy of a waaj and spic double deletion korean epidemic strain of salmonella enterica serovar gallinarum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/82ab8223aec842ec8059bdf0def0bc3d
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Evaluation of Safety and Protective Efficacy of a waaJ and spiC Double Deletion Korean Epidemic Strain of Salmonella enterica Serovar Gallinarum

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