“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid

Abstract Diagnosis of seizure disorders such as epilepsy currently relies on clinical examination and electroencephalogram recordings and is associated with substantial mis-diagnosis. The miRNA, miR-134 (MIR134 in humans), has been found to be elevated in brain tissue after experimental status epile...

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Autores principales:	Hazel McArdle, Eva M. Jimenez-Mateos, Rana Raoof, Eadaoin Carthy, David Boyle, Hany ElNaggar, Norman Delanty, Hajo Hamer, Muejgdan Dogan, Tessa Huchtemann, Peter Kӧrtvelyessy, Felix Rosenow, Robert J. Forster, David C. Henshall, Elaine Spain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82ad782d772448c6aa8e45ae4ebd31e4
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spelling	oai:doaj.org-article:82ad782d772448c6aa8e45ae4ebd31e42021-12-02T15:05:57Z“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid10.1038/s41598-017-01947-22045-2322https://doaj.org/article/82ad782d772448c6aa8e45ae4ebd31e42017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01947-2https://doaj.org/toc/2045-2322Abstract Diagnosis of seizure disorders such as epilepsy currently relies on clinical examination and electroencephalogram recordings and is associated with substantial mis-diagnosis. The miRNA, miR-134 (MIR134 in humans), has been found to be elevated in brain tissue after experimental status epilepticus and in human epilepsy cells and their detection in biofluids may serve as unique biomarkers. miRNAs from unprocessed human plasma and human cerebrospinal fluid samples were used in a novel electrochemical detection based on electrocatalytic platinum nanoparticles inside a centrifugal microfluidic device where the sandwich assay is formed using an event triggered release system, suitable for the rapid point-of-care detection of low abundance biomarkers of disease. The device has the advantage of controlling the rotation speed of the centrifugal device to pump nanoliter volumes of fluid at a set time and manipulate the transfer of liquids within the device. The centrifugal platform improves reaction rates and yields by proposing efficient mixing strategies to overcome diffusion-limited processes and improve mass transport rates, resulting in reduced hybridization times with a limit of detection of 1 pM target concentration. Plasma and cerebrospinal fluid samples (unprocessed) from patients with epilepsy or who experienced status epilepticus were tested and the catalytic response obtained was in range of the calibration plot. This study demonstrates a rapid and simple detection for epilepsy biomarkers in biofluid.Hazel McArdleEva M. Jimenez-MateosRana RaoofEadaoin CarthyDavid BoyleHany ElNaggarNorman DelantyHajo HamerMuejgdan DoganTessa HuchtemannPeter KӧrtvelyessyFelix RosenowRobert J. ForsterDavid C. HenshallElaine SpainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hazel McArdle Eva M. Jimenez-Mateos Rana Raoof Eadaoin Carthy David Boyle Hany ElNaggar Norman Delanty Hajo Hamer Muejgdan Dogan Tessa Huchtemann Peter Kӧrtvelyessy Felix Rosenow Robert J. Forster David C. Henshall Elaine Spain “TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid
description	Abstract Diagnosis of seizure disorders such as epilepsy currently relies on clinical examination and electroencephalogram recordings and is associated with substantial mis-diagnosis. The miRNA, miR-134 (MIR134 in humans), has been found to be elevated in brain tissue after experimental status epilepticus and in human epilepsy cells and their detection in biofluids may serve as unique biomarkers. miRNAs from unprocessed human plasma and human cerebrospinal fluid samples were used in a novel electrochemical detection based on electrocatalytic platinum nanoparticles inside a centrifugal microfluidic device where the sandwich assay is formed using an event triggered release system, suitable for the rapid point-of-care detection of low abundance biomarkers of disease. The device has the advantage of controlling the rotation speed of the centrifugal device to pump nanoliter volumes of fluid at a set time and manipulate the transfer of liquids within the device. The centrifugal platform improves reaction rates and yields by proposing efficient mixing strategies to overcome diffusion-limited processes and improve mass transport rates, resulting in reduced hybridization times with a limit of detection of 1 pM target concentration. Plasma and cerebrospinal fluid samples (unprocessed) from patients with epilepsy or who experienced status epilepticus were tested and the catalytic response obtained was in range of the calibration plot. This study demonstrates a rapid and simple detection for epilepsy biomarkers in biofluid.
format	article
author	Hazel McArdle Eva M. Jimenez-Mateos Rana Raoof Eadaoin Carthy David Boyle Hany ElNaggar Norman Delanty Hajo Hamer Muejgdan Dogan Tessa Huchtemann Peter Kӧrtvelyessy Felix Rosenow Robert J. Forster David C. Henshall Elaine Spain
author_facet	Hazel McArdle Eva M. Jimenez-Mateos Rana Raoof Eadaoin Carthy David Boyle Hany ElNaggar Norman Delanty Hajo Hamer Muejgdan Dogan Tessa Huchtemann Peter Kӧrtvelyessy Felix Rosenow Robert J. Forster David C. Henshall Elaine Spain
author_sort	Hazel McArdle
title	“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid
title_short	“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid
title_full	“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid
title_fullStr	“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid
title_full_unstemmed	“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid
title_sort	“tornado” – theranostic one-step rna detector; microfluidic disc for the direct detection of microrna-134 in plasma and cerebrospinal fluid
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/82ad782d772448c6aa8e45ae4ebd31e4
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“TORNADO” – Theranostic One-Step RNA Detector; microfluidic disc for the direct detection of microRNA-134 in plasma and cerebrospinal fluid

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