Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds

Juliane Pasold,1 Kathleen Zander,1 Benjamin Heskamp,1 Cordula Grüttner,2 Frank Lüthen,3 Thomas Tischer,1 Anika Jonitz-Heincke,1 Rainer Bader1 1Department of Orthopaedics, Biomechanics and Implant Technology Laboratory, University Medicine Rostock, Rostock, Germany; 2Micromod Part...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pasold J, Zander K, Heskamp B, Grüttner C, Lüthen F, Tischer T, Jonitz-Heincke A, Bader R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/82af9365a74e437b9d82800b39ddf4b7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:82af9365a74e437b9d82800b39ddf4b7
record_format	dspace
spelling	oai:doaj.org-article:82af9365a74e437b9d82800b39ddf4b72021-12-02T02:10:31ZPositive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds1178-2013https://doaj.org/article/82af9365a74e437b9d82800b39ddf4b72015-02-01T00:00:00Zhttp://www.dovepress.com/positive-impact-of-igf-1-coupled-nanoparticles-on-the-differentiation--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Juliane Pasold,1 Kathleen Zander,1 Benjamin Heskamp,1 Cordula Grüttner,2 Frank Lüthen,3 Thomas Tischer,1 Anika Jonitz-Heincke,1 Rainer Bader1 1Department of Orthopaedics, Biomechanics and Implant Technology Laboratory, University Medicine Rostock, Rostock, Germany; 2Micromod Particletechnology GmbH, Rostock, Germany; 3Institute of Cell Biology, University Medicine Rostock, Rostock, Germany Purpose: In the present study, silica nanoparticles (sNP) coupled with insulin-like growth factor 1 (IGF-1) were loaded on a collagen-based scaffold intended for cartilage repair, and the influence on the viability, proliferation, and differentiation potential of human primary articular chondrocytes was examined. Methods: Human chondrocytes were isolated from the hyaline cartilage of patients (n=4, female, mean age: 73±5.1 years) undergoing primary total knee joint replacement. Cells were dedifferentiated and then cultivated on a bioresorbable collagen matrix supplemented with fluorescent sNP coupled with IGF-1 (sNP–IGF-1). After 3, 7, and 14 days of cultivation, cell viability and integrity into the collagen scaffold as well as metabolic cell activity and synthesis rate of matrix proteins (collagen type I and II) were analyzed. Results: The number of vital cells increased over 14 days of cultivation, and the cells were able to infiltrate the collagen matrix (up to 120 µm by day 7). Chondrocytes cultured on the collagen scaffold supplemented with sNP–IGF-1 showed an increase in metabolic activity (5.98-fold), and reduced collagen type I (1.58-fold), but significantly increased collagen type II expression levels (1.53-fold; P=0.02) after 7 days of cultivation compared to 3 days. In contrast, chondrocytes grown in a monolayer on plastic supplemented with sNP-IGF-1 had significantly lower metabolic activity (1.32-fold; P=0.007), a consistent amount of collagen type I, and significantly reduced collagen type II protein expression (1.86-fold; P=0.001) after 7 days compared to 3 days. Conclusion: Collagen-based scaffolds enriched with growth factors, such as IGF-1 coupled to nanoparticles, represent an improved therapeutic intervention for the targeted and controlled treatment of articular cartilage lesions. Keywords: chondrogenic differentiation, silica nanoparticles, growth factor, 3D-matrix, cartilage repairPasold JZander KHeskamp BGrüttner CLüthen FTischer TJonitz-Heincke ABader RDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1131-1143 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Pasold J Zander K Heskamp B Grüttner C Lüthen F Tischer T Jonitz-Heincke A Bader R Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
description	Juliane Pasold,1 Kathleen Zander,1 Benjamin Heskamp,1 Cordula Grüttner,2 Frank Lüthen,3 Thomas Tischer,1 Anika Jonitz-Heincke,1 Rainer Bader1 1Department of Orthopaedics, Biomechanics and Implant Technology Laboratory, University Medicine Rostock, Rostock, Germany; 2Micromod Particletechnology GmbH, Rostock, Germany; 3Institute of Cell Biology, University Medicine Rostock, Rostock, Germany Purpose: In the present study, silica nanoparticles (sNP) coupled with insulin-like growth factor 1 (IGF-1) were loaded on a collagen-based scaffold intended for cartilage repair, and the influence on the viability, proliferation, and differentiation potential of human primary articular chondrocytes was examined. Methods: Human chondrocytes were isolated from the hyaline cartilage of patients (n=4, female, mean age: 73±5.1 years) undergoing primary total knee joint replacement. Cells were dedifferentiated and then cultivated on a bioresorbable collagen matrix supplemented with fluorescent sNP coupled with IGF-1 (sNP–IGF-1). After 3, 7, and 14 days of cultivation, cell viability and integrity into the collagen scaffold as well as metabolic cell activity and synthesis rate of matrix proteins (collagen type I and II) were analyzed. Results: The number of vital cells increased over 14 days of cultivation, and the cells were able to infiltrate the collagen matrix (up to 120 µm by day 7). Chondrocytes cultured on the collagen scaffold supplemented with sNP–IGF-1 showed an increase in metabolic activity (5.98-fold), and reduced collagen type I (1.58-fold), but significantly increased collagen type II expression levels (1.53-fold; P=0.02) after 7 days of cultivation compared to 3 days. In contrast, chondrocytes grown in a monolayer on plastic supplemented with sNP-IGF-1 had significantly lower metabolic activity (1.32-fold; P=0.007), a consistent amount of collagen type I, and significantly reduced collagen type II protein expression (1.86-fold; P=0.001) after 7 days compared to 3 days. Conclusion: Collagen-based scaffolds enriched with growth factors, such as IGF-1 coupled to nanoparticles, represent an improved therapeutic intervention for the targeted and controlled treatment of articular cartilage lesions. Keywords: chondrogenic differentiation, silica nanoparticles, growth factor, 3D-matrix, cartilage repair
format	article
author	Pasold J Zander K Heskamp B Grüttner C Lüthen F Tischer T Jonitz-Heincke A Bader R
author_facet	Pasold J Zander K Heskamp B Grüttner C Lüthen F Tischer T Jonitz-Heincke A Bader R
author_sort	Pasold J
title	Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
title_short	Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
title_full	Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
title_fullStr	Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
title_full_unstemmed	Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
title_sort	positive impact of igf-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/82af9365a74e437b9d82800b39ddf4b7
work_keys_str_mv	AT pasoldj positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT zanderk positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT heskampb positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT gruumlttnerc positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT luumlthenf positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT tischert positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT jonitzheinckea positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds AT baderr positiveimpactofigf1couplednanoparticlesonthedifferentiationpotentialofhumanchondrocytesculturedoncollagenscaffolds
_version_	1718402658727886848

Positive impact of IGF-1-coupled nanoparticles on the differentiation potential of human chondrocytes cultured on collagen scaffolds

Ejemplares similares