Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples

Abstract Oxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study human and animal behaviour with minimal disturbance. However, a thorough validation of ana...

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Autores principales:	G. Wirobski, F. S. Schaebs, F. Range, S. Marshall-Pescini, T. Deschner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82b6ea6828b84feda7ddc8e106d405b6
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spelling	oai:doaj.org-article:82b6ea6828b84feda7ddc8e106d405b62021-12-02T17:41:30ZAnalytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples10.1038/s41598-021-92356-z2045-2322https://doaj.org/article/82b6ea6828b84feda7ddc8e106d405b62021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92356-zhttps://doaj.org/toc/2045-2322Abstract Oxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study human and animal behaviour with minimal disturbance. However, a thorough validation of analytical methods and an assessment of the physiological significance of these measures are essential. We conducted an analytical validation of a commercial Enzyme Immunoassay (EIA; Arbor OT assay kit) to measure OTM concentrations in dog, wolf, and human urine samples. To test the assay’s ability to detect changes in OTM concentrations, we administered oxytocin intranasally to 14 dogs. Assay performance with regard to parallelism was acceptable. Assay accuracy and extraction efficiency for dog and wolf samples were comparable to a previously validated assay (Enzo OT assay kit) but variation was smaller for human samples. Binding sensitivity and antibody specificity were better in the Arbor assay. Average OTM concentrations were more than twice as high as in comparable samples measured with the Enzo assay, highlighting a lack of comparability of absolute values between different assays. Changes in OTM concentrations after intranasal treatment were detected reliably. The Arbor assay met requirements of a “fit-for-purpose” validation with improvement of several parameters compared to the Enzo assay.G. WirobskiF. S. SchaebsF. RangeS. Marshall-PesciniT. DeschnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q G. Wirobski F. S. Schaebs F. Range S. Marshall-Pescini T. Deschner Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
description	Abstract Oxytocin (OT) promotes pro-sociality, bonding, and cooperation in a variety of species. Measuring oxytocin metabolite (OTM) concentrations in urine or saliva provides intriguing opportunities to study human and animal behaviour with minimal disturbance. However, a thorough validation of analytical methods and an assessment of the physiological significance of these measures are essential. We conducted an analytical validation of a commercial Enzyme Immunoassay (EIA; Arbor OT assay kit) to measure OTM concentrations in dog, wolf, and human urine samples. To test the assay’s ability to detect changes in OTM concentrations, we administered oxytocin intranasally to 14 dogs. Assay performance with regard to parallelism was acceptable. Assay accuracy and extraction efficiency for dog and wolf samples were comparable to a previously validated assay (Enzo OT assay kit) but variation was smaller for human samples. Binding sensitivity and antibody specificity were better in the Arbor assay. Average OTM concentrations were more than twice as high as in comparable samples measured with the Enzo assay, highlighting a lack of comparability of absolute values between different assays. Changes in OTM concentrations after intranasal treatment were detected reliably. The Arbor assay met requirements of a “fit-for-purpose” validation with improvement of several parameters compared to the Enzo assay.
format	article
author	G. Wirobski F. S. Schaebs F. Range S. Marshall-Pescini T. Deschner
author_facet	G. Wirobski F. S. Schaebs F. Range S. Marshall-Pescini T. Deschner
author_sort	G. Wirobski
title	Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
title_short	Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
title_full	Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
title_fullStr	Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
title_full_unstemmed	Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
title_sort	analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/82b6ea6828b84feda7ddc8e106d405b6
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Analytical and physiological validation of an enzyme immunoassay to measure oxytocin in dog, wolf, and human urine samples

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