Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages

Trypanosoma cruzi is a protozoan parasite that affects millions of people in Latin America. Infection occurs by vectorial transmission or by transfusion or transplacental route. Immune events occurring immediately after the parasite entrance are poorly explored. Dendritic cells (DCs) are target for...

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Autores principales:	Brenda Celeste Gutierrez, Estela Lammel, Stella Maris González-Cappa, Carolina Verónica Poncini
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	T. cruzi parasite stages dendritic cells cell activation T cells Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/82b93a9165244a93b0df0c49ebcd4704
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spelling	oai:doaj.org-article:82b93a9165244a93b0df0c49ebcd47042021-12-01T02:01:42ZEarly Immune Response Elicited by Different Trypanosoma cruzi Infective Stages2235-298810.3389/fcimb.2021.768566https://doaj.org/article/82b93a9165244a93b0df0c49ebcd47042021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.768566/fullhttps://doaj.org/toc/2235-2988Trypanosoma cruzi is a protozoan parasite that affects millions of people in Latin America. Infection occurs by vectorial transmission or by transfusion or transplacental route. Immune events occurring immediately after the parasite entrance are poorly explored. Dendritic cells (DCs) are target for the parasite immune evasion mechanisms. Recently, we have demonstrated that two different populations of DCs display variable activation after interaction with the two infective forms of the parasite: metacyclic or blood trypomastigotes (mTp or bTp) in vitro. The skin constitutes a complex network with several populations of antigen-presenting cells. Previously, we have demonstrated T. cruzi conditioning the repertoire of cells recruited into the site of infection. In the present work, we observed that mTp and bTp inoculation displayed differences in cell recruitment to the site of infection and in the activation status of APCs in draining lymph nodes and spleen during acute infection. Animals inoculated with mTp exhibited 100% of survival with no detectable parasitemia, in contrast with those injected with bTp that displayed high mortality and high parasite load. Animals infected with mTp and challenged with a lethal dose of bTp 15 days after primary infection showed no mortality and incremented DC activation in secondary lymphoid organs compared with controls injected only with bTp or non-infected mice. These animals also displayed a smaller number of amastigote nests in cardiac tissue and more CD8 T cells than mice infected with bTp. All the results suggest that both Tp infective stages induce an unequal immune response since the beginning of the infection.Brenda Celeste GutierrezEstela LammelStella Maris González-CappaStella Maris González-CappaCarolina Verónica PonciniCarolina Verónica PonciniFrontiers Media S.A.articleT. cruziparasite stagesdendritic cellscell activationT cellsMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	T. cruzi parasite stages dendritic cells cell activation T cells Microbiology QR1-502
spellingShingle	T. cruzi parasite stages dendritic cells cell activation T cells Microbiology QR1-502 Brenda Celeste Gutierrez Estela Lammel Stella Maris González-Cappa Stella Maris González-Cappa Carolina Verónica Poncini Carolina Verónica Poncini Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages
description	Trypanosoma cruzi is a protozoan parasite that affects millions of people in Latin America. Infection occurs by vectorial transmission or by transfusion or transplacental route. Immune events occurring immediately after the parasite entrance are poorly explored. Dendritic cells (DCs) are target for the parasite immune evasion mechanisms. Recently, we have demonstrated that two different populations of DCs display variable activation after interaction with the two infective forms of the parasite: metacyclic or blood trypomastigotes (mTp or bTp) in vitro. The skin constitutes a complex network with several populations of antigen-presenting cells. Previously, we have demonstrated T. cruzi conditioning the repertoire of cells recruited into the site of infection. In the present work, we observed that mTp and bTp inoculation displayed differences in cell recruitment to the site of infection and in the activation status of APCs in draining lymph nodes and spleen during acute infection. Animals inoculated with mTp exhibited 100% of survival with no detectable parasitemia, in contrast with those injected with bTp that displayed high mortality and high parasite load. Animals infected with mTp and challenged with a lethal dose of bTp 15 days after primary infection showed no mortality and incremented DC activation in secondary lymphoid organs compared with controls injected only with bTp or non-infected mice. These animals also displayed a smaller number of amastigote nests in cardiac tissue and more CD8 T cells than mice infected with bTp. All the results suggest that both Tp infective stages induce an unequal immune response since the beginning of the infection.
format	article
author	Brenda Celeste Gutierrez Estela Lammel Stella Maris González-Cappa Stella Maris González-Cappa Carolina Verónica Poncini Carolina Verónica Poncini
author_facet	Brenda Celeste Gutierrez Estela Lammel Stella Maris González-Cappa Stella Maris González-Cappa Carolina Verónica Poncini Carolina Verónica Poncini
author_sort	Brenda Celeste Gutierrez
title	Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages
title_short	Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages
title_full	Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages
title_fullStr	Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages
title_full_unstemmed	Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages
title_sort	early immune response elicited by different trypanosoma cruzi infective stages
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/82b93a9165244a93b0df0c49ebcd4704
work_keys_str_mv	AT brendacelestegutierrez earlyimmuneresponseelicitedbydifferenttrypanosomacruziinfectivestages AT estelalammel earlyimmuneresponseelicitedbydifferenttrypanosomacruziinfectivestages AT stellamarisgonzalezcappa earlyimmuneresponseelicitedbydifferenttrypanosomacruziinfectivestages AT stellamarisgonzalezcappa earlyimmuneresponseelicitedbydifferenttrypanosomacruziinfectivestages AT carolinaveronicaponcini earlyimmuneresponseelicitedbydifferenttrypanosomacruziinfectivestages AT carolinaveronicaponcini earlyimmuneresponseelicitedbydifferenttrypanosomacruziinfectivestages
_version_	1718405971886211072

Early Immune Response Elicited by Different Trypanosoma cruzi Infective Stages

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