Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).

<h4>Background</h4>The insect midgut and fat body represent major tissue interfaces that deal with several important physiological functions including digestion, detoxification and immune response. The emerald ash borer (Agrilus planipennis), is an exotic invasive insect pest that has ki...

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Autores principales:	Omprakash Mittapalli, Xiaodong Bai, Praveen Mamidala, Swapna Priya Rajarapu, Pierluigi Bonello, Daniel A Herms
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82bc0890f614460d96be031cc9a1d97f
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spelling	oai:doaj.org-article:82bc0890f614460d96be031cc9a1d97f2021-11-18T07:02:43ZTissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).1932-620310.1371/journal.pone.0013708https://doaj.org/article/82bc0890f614460d96be031cc9a1d97f2010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21060843/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The insect midgut and fat body represent major tissue interfaces that deal with several important physiological functions including digestion, detoxification and immune response. The emerald ash borer (Agrilus planipennis), is an exotic invasive insect pest that has killed millions of ash trees (Fraxinus spp.) primarily in the Midwestern United States and Ontario, Canada. However, despite its high impact status little knowledge exists for A. planipennis at the molecular level.<h4>Methodology and principal findings</h4>Newer-generation Roche-454 pyrosequencing was used to obtain 126,185 reads for the midgut and 240,848 reads for the fat body, which were assembled into 25,173 and 37,661 high quality expressed sequence tags (ESTs) for the midgut and the fat body of A. planipennis larvae, respectively. Among these ESTs, 36% of the midgut and 38% of the fat body sequences showed similarity to proteins in the GenBank nr database. A high number of the midgut sequences contained chitin-binding peritrophin (248)and trypsin (98) domains; while the fat body sequences showed high occurrence of cytochrome P450s (85) and protein kinase (123) domains. Further, the midgut transcriptome of A. planipennis revealed putative microbial transcripts encoding for cell-wall degrading enzymes such as polygalacturonases and endoglucanases. A significant number of SNPs (137 in midgut and 347 in fat body) and microsatellite loci (317 in midgut and 571 in fat body) were predicted in the A. planipennis transcripts. An initial assessment of cytochrome P450s belonging to various CYP clades revealed distinct expression patterns at the tissue level.<h4>Conclusions and significance</h4>To our knowledge this study is one of the first to illuminate tissue-specific gene expression in an invasive insect of high ecological and economic consequence. These findings will lay the foundation for future gene expression and functional studies in A. planipennis.Omprakash MittapalliXiaodong BaiPraveen MamidalaSwapna Priya RajarapuPierluigi BonelloDaniel A HermsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13708 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Omprakash Mittapalli Xiaodong Bai Praveen Mamidala Swapna Priya Rajarapu Pierluigi Bonello Daniel A Herms Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).
description	<h4>Background</h4>The insect midgut and fat body represent major tissue interfaces that deal with several important physiological functions including digestion, detoxification and immune response. The emerald ash borer (Agrilus planipennis), is an exotic invasive insect pest that has killed millions of ash trees (Fraxinus spp.) primarily in the Midwestern United States and Ontario, Canada. However, despite its high impact status little knowledge exists for A. planipennis at the molecular level.<h4>Methodology and principal findings</h4>Newer-generation Roche-454 pyrosequencing was used to obtain 126,185 reads for the midgut and 240,848 reads for the fat body, which were assembled into 25,173 and 37,661 high quality expressed sequence tags (ESTs) for the midgut and the fat body of A. planipennis larvae, respectively. Among these ESTs, 36% of the midgut and 38% of the fat body sequences showed similarity to proteins in the GenBank nr database. A high number of the midgut sequences contained chitin-binding peritrophin (248)and trypsin (98) domains; while the fat body sequences showed high occurrence of cytochrome P450s (85) and protein kinase (123) domains. Further, the midgut transcriptome of A. planipennis revealed putative microbial transcripts encoding for cell-wall degrading enzymes such as polygalacturonases and endoglucanases. A significant number of SNPs (137 in midgut and 347 in fat body) and microsatellite loci (317 in midgut and 571 in fat body) were predicted in the A. planipennis transcripts. An initial assessment of cytochrome P450s belonging to various CYP clades revealed distinct expression patterns at the tissue level.<h4>Conclusions and significance</h4>To our knowledge this study is one of the first to illuminate tissue-specific gene expression in an invasive insect of high ecological and economic consequence. These findings will lay the foundation for future gene expression and functional studies in A. planipennis.
format	article
author	Omprakash Mittapalli Xiaodong Bai Praveen Mamidala Swapna Priya Rajarapu Pierluigi Bonello Daniel A Herms
author_facet	Omprakash Mittapalli Xiaodong Bai Praveen Mamidala Swapna Priya Rajarapu Pierluigi Bonello Daniel A Herms
author_sort	Omprakash Mittapalli
title	Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).
title_short	Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).
title_full	Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).
title_fullStr	Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).
title_full_unstemmed	Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).
title_sort	tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (agrilus planipennis).
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/82bc0890f614460d96be031cc9a1d97f
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Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).

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