Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis

Abstract Emerging evidence supports that stem cells are regulated by both intrinsic and extrinsic mechanisms. However, factors that determine the fate of stem cells remain incompletely understood. The Drosophila testis provides an exclusive powerful model in searching for potential important regulat...

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Autores principales:	Dongsheng Chen, Xiangxiang Zhu, Lijuan Zhou, Jian Wang, Xiaoqian Tao, Shuang Wang, Fuling Sun, Xianzhao Kan, Zhengqi Han, Yuelin Gu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82bc8fdfb0f14cc5af49ec463916e3e3
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spelling	oai:doaj.org-article:82bc8fdfb0f14cc5af49ec463916e3e32021-12-02T16:07:56ZGilgamesh is required for the maintenance of germline stem cells in Drosophila testis10.1038/s41598-017-05975-w2045-2322https://doaj.org/article/82bc8fdfb0f14cc5af49ec463916e3e32017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05975-whttps://doaj.org/toc/2045-2322Abstract Emerging evidence supports that stem cells are regulated by both intrinsic and extrinsic mechanisms. However, factors that determine the fate of stem cells remain incompletely understood. The Drosophila testis provides an exclusive powerful model in searching for potential important regulatory factors and their underlying mechanisms for controlling the fate of germline stem cells (GSCs). In this study, we have found that Drosophila gilgamesh (gish), which encodes a homologue of human CK1-γ (casein kinase 1-gamma), is required intrinsically for GSC maintenance. Our genetic analyses indicate gish is not required for Dpp/Gbb signaling silencing of bam and is dispensable for Dpp/Gbb signaling-dependent Dad expression. Finally, we show that overexpression of gish fail to dramatically increase the number of GSCs. These findings demonstrate that gish controls the fate of GSCs in Drosophila testis by a novel Dpp/Gbb signaling-independent pathway.Dongsheng ChenXiangxiang ZhuLijuan ZhouJian WangXiaoqian TaoShuang WangFuling SunXianzhao KanZhengqi HanYuelin GuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dongsheng Chen Xiangxiang Zhu Lijuan Zhou Jian Wang Xiaoqian Tao Shuang Wang Fuling Sun Xianzhao Kan Zhengqi Han Yuelin Gu Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis
description	Abstract Emerging evidence supports that stem cells are regulated by both intrinsic and extrinsic mechanisms. However, factors that determine the fate of stem cells remain incompletely understood. The Drosophila testis provides an exclusive powerful model in searching for potential important regulatory factors and their underlying mechanisms for controlling the fate of germline stem cells (GSCs). In this study, we have found that Drosophila gilgamesh (gish), which encodes a homologue of human CK1-γ (casein kinase 1-gamma), is required intrinsically for GSC maintenance. Our genetic analyses indicate gish is not required for Dpp/Gbb signaling silencing of bam and is dispensable for Dpp/Gbb signaling-dependent Dad expression. Finally, we show that overexpression of gish fail to dramatically increase the number of GSCs. These findings demonstrate that gish controls the fate of GSCs in Drosophila testis by a novel Dpp/Gbb signaling-independent pathway.
format	article
author	Dongsheng Chen Xiangxiang Zhu Lijuan Zhou Jian Wang Xiaoqian Tao Shuang Wang Fuling Sun Xianzhao Kan Zhengqi Han Yuelin Gu
author_facet	Dongsheng Chen Xiangxiang Zhu Lijuan Zhou Jian Wang Xiaoqian Tao Shuang Wang Fuling Sun Xianzhao Kan Zhengqi Han Yuelin Gu
author_sort	Dongsheng Chen
title	Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis
title_short	Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis
title_full	Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis
title_fullStr	Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis
title_full_unstemmed	Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis
title_sort	gilgamesh is required for the maintenance of germline stem cells in drosophila testis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/82bc8fdfb0f14cc5af49ec463916e3e3
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Gilgamesh is required for the maintenance of germline stem cells in Drosophila testis

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