Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.

Dendritic cells are the professional antigen presenting cells of innate immunity and key players in maintaining the balance of immune responses. Studies with dendritic cells are mainly limited by their low numbers in vivo and their difficult maintenance in vitro. We differentiated bone marrow cells...

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Autores principales:	Cornelia Richter, Sebastian Thieme, Joanna Bandoła, Magdalena Laugsch, Konstantinos Anastassiadis, Sebastian Brenner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82bf1a9219a5402883a80c53fdc03da9
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spelling	oai:doaj.org-article:82bf1a9219a5402883a80c53fdc03da92021-11-18T07:48:14ZGeneration of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.1932-620310.1371/journal.pone.0062621https://doaj.org/article/82bf1a9219a5402883a80c53fdc03da92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23626840/?tool=EBIhttps://doaj.org/toc/1932-6203Dendritic cells are the professional antigen presenting cells of innate immunity and key players in maintaining the balance of immune responses. Studies with dendritic cells are mainly limited by their low numbers in vivo and their difficult maintenance in vitro. We differentiated bone marrow cells from transgenic mice expressing an inducible SV40 large T-antigen into dendritic cells. When immortalized by dexamethasone and doxycycline, these cells were stable in long-term culture. In the absence of dexamethasone and doxycycline (de-induction), dendritic cells displayed properties of primary cells, characterized by expression of classical dendritic cell surface markers CD11c, CD11b, MHCII, CD40 and CD86. Furthermore, de-induced lipopolysaccharide activated dendritic cells secreted IL-1β, IL-6, TNFα and IL-12. De-induced, Ovalbumin-loaded dendritic cells polarize CD4(+) T cells into Th1, Th17 and Th2 cells, indicating their correct antigen presenting property. Consistent with intratracheal application of Ovalbumin-loaded primary dendritic cells into mice, the application of de-induced dendritic cells resulted in recruitment of lymphocytes to the lungs. In summary, we successfully expanded dendritic cells using conditional immortalization. The generated dendritic cells demonstrate the characteristic immunophenotype of primary dendritic cells and will facilitate further studies on immunomodulatory properties of dendritic cells.Cornelia RichterSebastian ThiemeJoanna BandołaMagdalena LaugschKonstantinos AnastassiadisSebastian BrennerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62621 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cornelia Richter Sebastian Thieme Joanna Bandoła Magdalena Laugsch Konstantinos Anastassiadis Sebastian Brenner Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
description	Dendritic cells are the professional antigen presenting cells of innate immunity and key players in maintaining the balance of immune responses. Studies with dendritic cells are mainly limited by their low numbers in vivo and their difficult maintenance in vitro. We differentiated bone marrow cells from transgenic mice expressing an inducible SV40 large T-antigen into dendritic cells. When immortalized by dexamethasone and doxycycline, these cells were stable in long-term culture. In the absence of dexamethasone and doxycycline (de-induction), dendritic cells displayed properties of primary cells, characterized by expression of classical dendritic cell surface markers CD11c, CD11b, MHCII, CD40 and CD86. Furthermore, de-induced lipopolysaccharide activated dendritic cells secreted IL-1β, IL-6, TNFα and IL-12. De-induced, Ovalbumin-loaded dendritic cells polarize CD4(+) T cells into Th1, Th17 and Th2 cells, indicating their correct antigen presenting property. Consistent with intratracheal application of Ovalbumin-loaded primary dendritic cells into mice, the application of de-induced dendritic cells resulted in recruitment of lymphocytes to the lungs. In summary, we successfully expanded dendritic cells using conditional immortalization. The generated dendritic cells demonstrate the characteristic immunophenotype of primary dendritic cells and will facilitate further studies on immunomodulatory properties of dendritic cells.
format	article
author	Cornelia Richter Sebastian Thieme Joanna Bandoła Magdalena Laugsch Konstantinos Anastassiadis Sebastian Brenner
author_facet	Cornelia Richter Sebastian Thieme Joanna Bandoła Magdalena Laugsch Konstantinos Anastassiadis Sebastian Brenner
author_sort	Cornelia Richter
title	Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
title_short	Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
title_full	Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
title_fullStr	Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
title_full_unstemmed	Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
title_sort	generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/82bf1a9219a5402883a80c53fdc03da9
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Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.

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