Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties

Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recomb...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	María José Diéguez, Micaela López, Emiliano Altieri, María Fernanda Pergolesi, Marisol Alicia Dabove, Alba Romina Cuyeu, Nadia Justus, Mariana Kandus, Lorena Ingala, Francisco Sacco
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	wheat leaf rust durable resistance adult plant resistance genes marker-assisted selection gene introgression fine mapping Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/82c0993937684269a8bd0ba301b43a94
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:82c0993937684269a8bd0ba301b43a94
record_format	dspace
spelling	oai:doaj.org-article:82c0993937684269a8bd0ba301b43a942021-11-25T16:47:38ZCharacterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties10.3390/biology101111682079-7737https://doaj.org/article/82c0993937684269a8bd0ba301b43a942021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1168https://doaj.org/toc/2079-7737Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene <i>LrBMP1</i> was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene <i>LrSV1</i>, the APR genetic system <i>LrSV2</i> + <i>LrcSV2</i> and the ASR (All Stage Resistance) gene <i>LrG6</i>. Using backcrosses, <i>LrBMP1</i> was introgressed in four commercial susceptible varieties and <i>LrSV1</i>, <i>LrSV2</i> + <i>LrcSV2</i> and <i>LrG6</i> were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system <i>LrSV2</i> + <i>LrcSV2</i> for leaf rust APR is reported.María José DiéguezMicaela LópezEmiliano AltieriMaría Fernanda PergolesiMarisol Alicia DaboveAlba Romina CuyeuNadia JustusMariana KandusLorena IngalaFrancisco SaccoMDPI AGarticlewheat leaf rustdurable resistanceadult plant resistance genesmarker-assisted selectiongene introgressionfine mappingBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1168, p 1168 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wheat leaf rust durable resistance adult plant resistance genes marker-assisted selection gene introgression fine mapping Biology (General) QH301-705.5
spellingShingle	wheat leaf rust durable resistance adult plant resistance genes marker-assisted selection gene introgression fine mapping Biology (General) QH301-705.5 María José Diéguez Micaela López Emiliano Altieri María Fernanda Pergolesi Marisol Alicia Dabove Alba Romina Cuyeu Nadia Justus Mariana Kandus Lorena Ingala Francisco Sacco Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
description	Leaf rust is one of the most significant diseases of wheat worldwide. In Argentina, it is one of the main reasons for variety replacement that becomes susceptible after large-scale use. Some varieties showed durable resistance to this disease, including Buck Manantial and Sinvalocho MA. RILs (Recombinant Inbred Lines) were developed for each of these varieties and used in genetics studies to identify components of resistance, both in greenhouse inoculations using leaf rust races, and in field evaluations under natural population infections. In Buck Manantial, the APR gene <i>LrBMP1</i> was associated with resistance in field tests. In crosses involving Sinvalocho MA, four genes were previously identified and associated with resistance in field testing: APR (Adult Plant Resistance) gene <i>LrSV1</i>, the APR genetic system <i>LrSV2</i> + <i>LrcSV2</i> and the ASR (All Stage Resistance) gene <i>LrG6</i>. Using backcrosses, <i>LrBMP1</i> was introgressed in four commercial susceptible varieties and <i>LrSV1</i>, <i>LrSV2</i> + <i>LrcSV2</i> and <i>LrG6</i> were simultaneously introgressed in three susceptible commercial varieties. The use of molecular markers for recurrent parent background selection allowed us to select resistant lines with more than 80% similarity to commercial varieties. Additionally, progress towards positional cloning of the genetic system <i>LrSV2</i> + <i>LrcSV2</i> for leaf rust APR is reported.
format	article
author	María José Diéguez Micaela López Emiliano Altieri María Fernanda Pergolesi Marisol Alicia Dabove Alba Romina Cuyeu Nadia Justus Mariana Kandus Lorena Ingala Francisco Sacco
author_facet	María José Diéguez Micaela López Emiliano Altieri María Fernanda Pergolesi Marisol Alicia Dabove Alba Romina Cuyeu Nadia Justus Mariana Kandus Lorena Ingala Francisco Sacco
author_sort	María José Diéguez
title	Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
title_short	Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
title_full	Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
title_fullStr	Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
title_full_unstemmed	Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties
title_sort	characterization and use in wheat breeding of leaf rust resistance genes from durable varieties
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/82c0993937684269a8bd0ba301b43a94
work_keys_str_mv	AT mariajosedieguez characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT micaelalopez characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT emilianoaltieri characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT mariafernandapergolesi characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT marisolaliciadabove characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT albarominacuyeu characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT nadiajustus characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT marianakandus characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT lorenaingala characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties AT franciscosacco characterizationanduseinwheatbreedingofleafrustresistancegenesfromdurablevarieties
_version_	1718412966540345344

Characterization and Use in Wheat Breeding of Leaf Rust Resistance Genes from Durable Varieties

Ejemplares similares