Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.

The transcriptome profiles were compared for buffalo embryos with normal growth and embryos with retarded growth on Day 25 after mating. Embryos with retarded growth on Day 25 after mating have a reduced likelihood of undergoing attachment to the uterine endometrium and establishing a pregnancy. Ita...

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Autores principales:	Maria Strazzullo, Bianca Gasparrini, Gianluca Neglia, Maria Luisa Balestrieri, Romina Francioso, Cristina Rossetti, Giovanni Nassa, Maria Rosaria De Filippo, Alessandro Weisz, Serena Di Francesco, Domenico Vecchio, Maurizio D'Esposito, Michael John D'Occhio, Luigi Zicarelli, Giuseppe Campanile
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82cff6b239154b5db6c139128b24119e
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spelling	oai:doaj.org-article:82cff6b239154b5db6c139128b24119e2021-11-18T08:30:26ZGlobal transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.1932-620310.1371/journal.pone.0090027https://doaj.org/article/82cff6b239154b5db6c139128b24119e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587197/?tool=EBIhttps://doaj.org/toc/1932-6203The transcriptome profiles were compared for buffalo embryos with normal growth and embryos with retarded growth on Day 25 after mating. Embryos with retarded growth on Day 25 after mating have a reduced likelihood of undergoing attachment to the uterine endometrium and establishing a pregnancy. Italian Mediterranean buffaloes were mated by AI and on Day 25 underwent trans-rectal ultrasonography to ascertain embryo development. Embryos with an embryonic width (EW)>2.7 mm were classed as normal embryos and embryos with an EW<2.7 mm were classed as retarded embryos. Three buffaloes with embryos of the largest EW (3.7, 3.7 and 3.9 mm) and three buffaloes with embryos of the smallest EW (1.5, 1.6 and 1.9 mm) were slaughtered on Day 27 to recover embryos for transcriptome analysis using a bovine custom designed oligo array. A total of 1,047 transcripts were differentially expressed between embryos with normal growth and embryos with retarded growth. Retarded embryos showed 773/1,047 (74%) transcripts that were down-regulated and 274/1,047 (26%) transcripts that were up-regulated relative to normal embryos; in silico analyses focused on 680/1,047 (65%) of the differentially expressed transcripts. The most altered transcripts observed in retarded embryos were associated with membrane structure and function and with metabolic and homeostasis maintenance functions. Other notable functions altered in retarded embryos were developmental processes and in particular nervous system differentiation and function. Specific biochemical pathways such as the complement cascade and coagulation were also altered in retarded embryos. It was concluded from the findings that buffalo embryos with retarded growth on Day 25 after mating show altered gene expression compared with normal embryos, and some de-regulated functions are associated with attachment to the uterine endometrium.Maria StrazzulloBianca GasparriniGianluca NegliaMaria Luisa BalestrieriRomina FranciosoCristina RossettiGiovanni NassaMaria Rosaria De FilippoAlessandro WeiszSerena Di FrancescoDomenico VecchioMaurizio D'EspositoMichael John D'OcchioLuigi ZicarelliGiuseppe CampanilePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e90027 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Strazzullo Bianca Gasparrini Gianluca Neglia Maria Luisa Balestrieri Romina Francioso Cristina Rossetti Giovanni Nassa Maria Rosaria De Filippo Alessandro Weisz Serena Di Francesco Domenico Vecchio Maurizio D'Esposito Michael John D'Occhio Luigi Zicarelli Giuseppe Campanile Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.
description	The transcriptome profiles were compared for buffalo embryos with normal growth and embryos with retarded growth on Day 25 after mating. Embryos with retarded growth on Day 25 after mating have a reduced likelihood of undergoing attachment to the uterine endometrium and establishing a pregnancy. Italian Mediterranean buffaloes were mated by AI and on Day 25 underwent trans-rectal ultrasonography to ascertain embryo development. Embryos with an embryonic width (EW)>2.7 mm were classed as normal embryos and embryos with an EW<2.7 mm were classed as retarded embryos. Three buffaloes with embryos of the largest EW (3.7, 3.7 and 3.9 mm) and three buffaloes with embryos of the smallest EW (1.5, 1.6 and 1.9 mm) were slaughtered on Day 27 to recover embryos for transcriptome analysis using a bovine custom designed oligo array. A total of 1,047 transcripts were differentially expressed between embryos with normal growth and embryos with retarded growth. Retarded embryos showed 773/1,047 (74%) transcripts that were down-regulated and 274/1,047 (26%) transcripts that were up-regulated relative to normal embryos; in silico analyses focused on 680/1,047 (65%) of the differentially expressed transcripts. The most altered transcripts observed in retarded embryos were associated with membrane structure and function and with metabolic and homeostasis maintenance functions. Other notable functions altered in retarded embryos were developmental processes and in particular nervous system differentiation and function. Specific biochemical pathways such as the complement cascade and coagulation were also altered in retarded embryos. It was concluded from the findings that buffalo embryos with retarded growth on Day 25 after mating show altered gene expression compared with normal embryos, and some de-regulated functions are associated with attachment to the uterine endometrium.
format	article
author	Maria Strazzullo Bianca Gasparrini Gianluca Neglia Maria Luisa Balestrieri Romina Francioso Cristina Rossetti Giovanni Nassa Maria Rosaria De Filippo Alessandro Weisz Serena Di Francesco Domenico Vecchio Maurizio D'Esposito Michael John D'Occhio Luigi Zicarelli Giuseppe Campanile
author_facet	Maria Strazzullo Bianca Gasparrini Gianluca Neglia Maria Luisa Balestrieri Romina Francioso Cristina Rossetti Giovanni Nassa Maria Rosaria De Filippo Alessandro Weisz Serena Di Francesco Domenico Vecchio Maurizio D'Esposito Michael John D'Occhio Luigi Zicarelli Giuseppe Campanile
author_sort	Maria Strazzullo
title	Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.
title_short	Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.
title_full	Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.
title_fullStr	Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.
title_full_unstemmed	Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.
title_sort	global transcriptome profiles of italian mediterranean buffalo embryos with normal and retarded growth.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/82cff6b239154b5db6c139128b24119e
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Global transcriptome profiles of Italian Mediterranean buffalo embryos with normal and retarded growth.

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