Offspring production from cryopreserved primordial germ cells in Drosophila
Asaoka, Sakamaki, Fukumoto et al. present a more viable method of long-term storage of Drosophila fly strains by cryopreservation of primordial germ cells (PGCs) without any drop in viability. The authors show that PGCs from stage 5 embryos can be transplanted into embryos and properly developed int...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/82d699b101e344e6a70debccf44f84b8 |
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| Sumario: | Asaoka, Sakamaki, Fukumoto et al. present a more viable method of long-term storage of Drosophila fly strains by cryopreservation of primordial germ cells (PGCs) without any drop in viability. The authors show that PGCs from stage 5 embryos can be transplanted into embryos and properly developed into germline stem cells to produce offspring of both sexes after being revived from storage in liquid nitrogen. |
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