Offspring production from cryopreserved primordial germ cells in Drosophila

Asaoka, Sakamaki, Fukumoto et al. present a more viable method of long-term storage of Drosophila fly strains by cryopreservation of primordial germ cells (PGCs) without any drop in viability. The authors show that PGCs from stage 5 embryos can be transplanted into embryos and properly developed int...

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Autores principales:	Miho Asaoka, Yurina Sakamaki, Tatsuya Fukumoto, Kaori Nishimura, Masatoshi Tomaru, Toshiyuki Takano-Shimizu, Daisuke Tanaka, Satoru Kobayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/82d699b101e344e6a70debccf44f84b8
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spelling	oai:doaj.org-article:82d699b101e344e6a70debccf44f84b82021-12-02T19:16:17ZOffspring production from cryopreserved primordial germ cells in Drosophila10.1038/s42003-021-02692-z2399-3642https://doaj.org/article/82d699b101e344e6a70debccf44f84b82021-10-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02692-zhttps://doaj.org/toc/2399-3642Asaoka, Sakamaki, Fukumoto et al. present a more viable method of long-term storage of Drosophila fly strains by cryopreservation of primordial germ cells (PGCs) without any drop in viability. The authors show that PGCs from stage 5 embryos can be transplanted into embryos and properly developed into germline stem cells to produce offspring of both sexes after being revived from storage in liquid nitrogen.Miho AsaokaYurina SakamakiTatsuya FukumotoKaori NishimuraMasatoshi TomaruToshiyuki Takano-ShimizuDaisuke TanakaSatoru KobayashiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Miho Asaoka Yurina Sakamaki Tatsuya Fukumoto Kaori Nishimura Masatoshi Tomaru Toshiyuki Takano-Shimizu Daisuke Tanaka Satoru Kobayashi Offspring production from cryopreserved primordial germ cells in Drosophila
description	Asaoka, Sakamaki, Fukumoto et al. present a more viable method of long-term storage of Drosophila fly strains by cryopreservation of primordial germ cells (PGCs) without any drop in viability. The authors show that PGCs from stage 5 embryos can be transplanted into embryos and properly developed into germline stem cells to produce offspring of both sexes after being revived from storage in liquid nitrogen.
format	article
author	Miho Asaoka Yurina Sakamaki Tatsuya Fukumoto Kaori Nishimura Masatoshi Tomaru Toshiyuki Takano-Shimizu Daisuke Tanaka Satoru Kobayashi
author_facet	Miho Asaoka Yurina Sakamaki Tatsuya Fukumoto Kaori Nishimura Masatoshi Tomaru Toshiyuki Takano-Shimizu Daisuke Tanaka Satoru Kobayashi
author_sort	Miho Asaoka
title	Offspring production from cryopreserved primordial germ cells in Drosophila
title_short	Offspring production from cryopreserved primordial germ cells in Drosophila
title_full	Offspring production from cryopreserved primordial germ cells in Drosophila
title_fullStr	Offspring production from cryopreserved primordial germ cells in Drosophila
title_full_unstemmed	Offspring production from cryopreserved primordial germ cells in Drosophila
title_sort	offspring production from cryopreserved primordial germ cells in drosophila
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/82d699b101e344e6a70debccf44f84b8
work_keys_str_mv	AT mihoasaoka offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT yurinasakamaki offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT tatsuyafukumoto offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT kaorinishimura offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT masatoshitomaru offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT toshiyukitakanoshimizu offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT daisuketanaka offspringproductionfromcryopreservedprimordialgermcellsindrosophila AT satorukobayashi offspringproductionfromcryopreservedprimordialgermcellsindrosophila
_version_	1718376980791951360

Offspring production from cryopreserved primordial germ cells in Drosophila

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