$Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy$
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Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy

Abu S M Mohsin,1 Mariam B Salim2 1Centre for Micro-Photonics, Department of Physics, Swinburne University of Technology, Melbourne, VIC 3122, Australia; 2Electrical and Telecommunication Engineering, Swinburne University of Technology, Melbourne, VIC 3122, Australia Background: We have introduced...

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Autores principales:	Mohsin ASM, Salim MB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	intracellular refractive index intracellular biosensor molecular interaction single particle study Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/82d85ef8fafa42a6a2721c3bcf10d27c
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spelling	oai:doaj.org-article:82d85ef8fafa42a6a2721c3bcf10d27c2021-12-02T05:06:32ZProbing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy1178-2013https://doaj.org/article/82d85ef8fafa42a6a2721c3bcf10d27c2018-10-01T00:00:00Zhttps://www.dovepress.com/probing-the-intracellular-refractive-index-and-molecular-interaction-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Abu S M Mohsin,1 Mariam B Salim2 1Centre for Micro-Photonics, Department of Physics, Swinburne University of Technology, Melbourne, VIC 3122, Australia; 2Electrical and Telecommunication Engineering, Swinburne University of Technology, Melbourne, VIC 3122, Australia Background: We have introduced a novel method to quantify the intracellular refractive index (RI) of living cells and determine the molecular interaction of two interacting molecules using single particle spectroscopy. The advantages of this proposed technique over fluorescence-based imaging techniques is that it does not require any contrasting agent and it does not blink and bleach. Instead, our technique provides a non-destructive, non-invasive, high-resolution imaging of live cells.Methods: To verify our technique, we initially tested our approach for a dielectric medium where gold nanoparticles (AuNPs) were embedded in a polyvinyl alcohol (PVA) matrix, which was then extended to the cellular environment. In the dielectric medium, we identified the single particle and dimer and determined the interparticle distance of AuNPs using confocal laser scattering microscopy. We also determined the single particle RI from dark-field scattering microscopy images, which was confirmed with Mie theory and finite-difference time-domain (FDTD) simulated results. The single particle spectroscopy and microscopy technique was then extended to determine the intracellular RI and biomolecular interaction inside living cells using hyperspectral imaging and dark-field scattering microscopy.Results: The novelty of the paper lies in the demonstration of a direct and accurate method to probe the intracellular RI and molecular interaction focused on single particle analysis whereas previous demonstrations were based on AuNP ensembles. Optically acquired single particle and dimer images was verified by correlated SEM images also optical spectrum with analytical models and FDTD simulations for both the dielectric and cellular environment. We reported the interparticle distance of AuNPs inside HeLa cells and intracellular refractive index, which was also confirmed with Mie Theory and extensive FDTD simulations.Conclusion: Moreover, we believe that our in-depth plasmonic NP-based alternate imaging technique will provide a new insight in monitoring cellular dynamics and tracking the targeted NPs within live cells, enabling us to use plasmonic NPs as an intracellular biosensor. Keywords: intracellular refractive index, molecular interaction, dimerization, single particle spectroscopy, biosensorMohsin ASMSalim MBDove Medical Pressarticleintracellular refractive indexintracellular biosensormolecular interactionsingle particle studyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6019-6028 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	intracellular refractive index intracellular biosensor molecular interaction single particle study Medicine (General) R5-920
spellingShingle	intracellular refractive index intracellular biosensor molecular interaction single particle study Medicine (General) R5-920 Mohsin ASM Salim MB Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy
description	Abu S M Mohsin,1 Mariam B Salim2 1Centre for Micro-Photonics, Department of Physics, Swinburne University of Technology, Melbourne, VIC 3122, Australia; 2Electrical and Telecommunication Engineering, Swinburne University of Technology, Melbourne, VIC 3122, Australia Background: We have introduced a novel method to quantify the intracellular refractive index (RI) of living cells and determine the molecular interaction of two interacting molecules using single particle spectroscopy. The advantages of this proposed technique over fluorescence-based imaging techniques is that it does not require any contrasting agent and it does not blink and bleach. Instead, our technique provides a non-destructive, non-invasive, high-resolution imaging of live cells.Methods: To verify our technique, we initially tested our approach for a dielectric medium where gold nanoparticles (AuNPs) were embedded in a polyvinyl alcohol (PVA) matrix, which was then extended to the cellular environment. In the dielectric medium, we identified the single particle and dimer and determined the interparticle distance of AuNPs using confocal laser scattering microscopy. We also determined the single particle RI from dark-field scattering microscopy images, which was confirmed with Mie theory and finite-difference time-domain (FDTD) simulated results. The single particle spectroscopy and microscopy technique was then extended to determine the intracellular RI and biomolecular interaction inside living cells using hyperspectral imaging and dark-field scattering microscopy.Results: The novelty of the paper lies in the demonstration of a direct and accurate method to probe the intracellular RI and molecular interaction focused on single particle analysis whereas previous demonstrations were based on AuNP ensembles. Optically acquired single particle and dimer images was verified by correlated SEM images also optical spectrum with analytical models and FDTD simulations for both the dielectric and cellular environment. We reported the interparticle distance of AuNPs inside HeLa cells and intracellular refractive index, which was also confirmed with Mie Theory and extensive FDTD simulations.Conclusion: Moreover, we believe that our in-depth plasmonic NP-based alternate imaging technique will provide a new insight in monitoring cellular dynamics and tracking the targeted NPs within live cells, enabling us to use plasmonic NPs as an intracellular biosensor. Keywords: intracellular refractive index, molecular interaction, dimerization, single particle spectroscopy, biosensor
format	article
author	Mohsin ASM Salim MB
author_facet	Mohsin ASM Salim MB
author_sort	Mohsin ASM
title	Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy
title_short	Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy
title_full	Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy
title_fullStr	Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy
title_full_unstemmed	Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy
title_sort	probing the intracellular refractive index and molecular interaction of gold nanoparticle in hela cells using single particle spectroscopy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/82d85ef8fafa42a6a2721c3bcf10d27c
work_keys_str_mv	AT mohsinasm probingtheintracellularrefractiveindexandmolecularinteractionofgoldnanoparticleinhelacellsusingsingleparticlespectroscopy AT salimmb probingtheintracellularrefractiveindexandmolecularinteractionofgoldnanoparticleinhelacellsusingsingleparticlespectroscopy
_version_	1718400600956207104

Probing the intracellular refractive index and molecular interaction of gold nanoparticle in HeLa cells using single particle spectroscopy

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