Structural and functional identification of vasculogenic mimicry in vitro
Abstract Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in an endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of an in vitro model of VM. Furthermore,...
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Nature Portfolio
2017
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oai:doaj.org-article:82e3e7833ff24addbd71128e3485aed82021-12-02T15:06:13ZStructural and functional identification of vasculogenic mimicry in vitro10.1038/s41598-017-07622-w2045-2322https://doaj.org/article/82e3e7833ff24addbd71128e3485aed82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07622-whttps://doaj.org/toc/2045-2322Abstract Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in an endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of an in vitro model of VM. Furthermore, many studies that claim to demonstrate VM fail to provide solid evidence of true hollow channels, raising concerns as to whether actual VM is actually being examined. Herein, we provide a standardized in vitro assay that recreates the formation of functional hollow channels using ovarian cancer cell lines, cancer spheres and primary cultures derived from ovarian cancer ascites. X-ray microtomography 3D-reconstruction, fluorescence confocal microscopy and dye microinjection conclusively confirm the existence of functional glycoprotein-rich lined tubular structures in vitro and demonstrate that many of structures reported in the literature may not represent VM. This assay may be useful to design and test future VM-blocking anticancer therapies.Dusan RacordonAndrés ValdiviaGabriel MingoRafaela EricesRaúl AravenaFelice SantoroMaria Loreto BravoCarolina RamirezPamela GonzalezAlejandra SandovalAlfonso GonzálezClaudio RetamalMarcelo J. KoganSumie KatoMauricio A. CuelloGerman OsorioFrancisco NualartPedro AlvaresAraceli Gago-AriasDaniella FabriIgnacio EspinozaBeatriz SanchezAlejandro H. CorvalánMauricio P. PintoGareth I. OwenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Dusan Racordon Andrés Valdivia Gabriel Mingo Rafaela Erices Raúl Aravena Felice Santoro Maria Loreto Bravo Carolina Ramirez Pamela Gonzalez Alejandra Sandoval Alfonso González Claudio Retamal Marcelo J. Kogan Sumie Kato Mauricio A. Cuello German Osorio Francisco Nualart Pedro Alvares Araceli Gago-Arias Daniella Fabri Ignacio Espinoza Beatriz Sanchez Alejandro H. Corvalán Mauricio P. Pinto Gareth I. Owen Structural and functional identification of vasculogenic mimicry in vitro |
| description |
Abstract Vasculogenic mimicry (VM) describes a process by which cancer cells establish an alternative perfusion pathway in an endothelial cell-free manner. Despite its strong correlation with reduced patient survival, controversy still surrounds the existence of an in vitro model of VM. Furthermore, many studies that claim to demonstrate VM fail to provide solid evidence of true hollow channels, raising concerns as to whether actual VM is actually being examined. Herein, we provide a standardized in vitro assay that recreates the formation of functional hollow channels using ovarian cancer cell lines, cancer spheres and primary cultures derived from ovarian cancer ascites. X-ray microtomography 3D-reconstruction, fluorescence confocal microscopy and dye microinjection conclusively confirm the existence of functional glycoprotein-rich lined tubular structures in vitro and demonstrate that many of structures reported in the literature may not represent VM. This assay may be useful to design and test future VM-blocking anticancer therapies. |
| format |
article |
| author |
Dusan Racordon Andrés Valdivia Gabriel Mingo Rafaela Erices Raúl Aravena Felice Santoro Maria Loreto Bravo Carolina Ramirez Pamela Gonzalez Alejandra Sandoval Alfonso González Claudio Retamal Marcelo J. Kogan Sumie Kato Mauricio A. Cuello German Osorio Francisco Nualart Pedro Alvares Araceli Gago-Arias Daniella Fabri Ignacio Espinoza Beatriz Sanchez Alejandro H. Corvalán Mauricio P. Pinto Gareth I. Owen |
| author_facet |
Dusan Racordon Andrés Valdivia Gabriel Mingo Rafaela Erices Raúl Aravena Felice Santoro Maria Loreto Bravo Carolina Ramirez Pamela Gonzalez Alejandra Sandoval Alfonso González Claudio Retamal Marcelo J. Kogan Sumie Kato Mauricio A. Cuello German Osorio Francisco Nualart Pedro Alvares Araceli Gago-Arias Daniella Fabri Ignacio Espinoza Beatriz Sanchez Alejandro H. Corvalán Mauricio P. Pinto Gareth I. Owen |
| author_sort |
Dusan Racordon |
| title |
Structural and functional identification of vasculogenic mimicry in vitro |
| title_short |
Structural and functional identification of vasculogenic mimicry in vitro |
| title_full |
Structural and functional identification of vasculogenic mimicry in vitro |
| title_fullStr |
Structural and functional identification of vasculogenic mimicry in vitro |
| title_full_unstemmed |
Structural and functional identification of vasculogenic mimicry in vitro |
| title_sort |
structural and functional identification of vasculogenic mimicry in vitro |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/82e3e7833ff24addbd71128e3485aed8 |
| work_keys_str_mv |
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