Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm

<i>Vitis vinifera</i> canes are waste material of grapevine pruning and thus represent cheap source of high-value polyphenols. In view of the fact that resistance of many pathogenic microorganisms to antibiotics is a growing problem, the antimicrobial activity of plant polyphenols is stu...

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Autores principales:	Zdeněk Kodeš, Maria Vrublevskaya, Markéta Kulišová, Petr Jaroš, Martina Paldrychová, Karolína Pádrová, Kristýna Lokočová, Andrea Palyzová, Olga Maťátková, Irena Kolouchová
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	resveratrol waste product utilization prevention of biofilm formation antioxidant activity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/82e93e545ce04bc29b9b37feb10777f2
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spelling	oai:doaj.org-article:82e93e545ce04bc29b9b37feb10777f22021-11-25T18:25:37ZComposition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm10.3390/microorganisms91123912076-2607https://doaj.org/article/82e93e545ce04bc29b9b37feb10777f22021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2391https://doaj.org/toc/2076-2607<i>Vitis vinifera</i> canes are waste material of grapevine pruning and thus represent cheap source of high-value polyphenols. In view of the fact that resistance of many pathogenic microorganisms to antibiotics is a growing problem, the antimicrobial activity of plant polyphenols is studied as one of the possible approaches. We have investigated the total phenolic content, composition, antioxidant activity, and antifungal activity against <i>Candida</i> biofilm of an extract from winter canes and a commercially available extract from blue grapes. Light microscopy and confocal microscopy imaging as well as crystal violet staining were used to quantify and visualize the biofilm. We found a decrease in cell adhesion to the surface depending on the concentration of resveratrol in the cane extract. The biofilm formation was observed as metabolic activity of <i>Candida albicans</i>, <i>Candida parapsilosis</i> and <i>Candida krusei</i> biofilm cells and the minimum biofilm inhibitory concentrations were determined. The highest inhibition of metabolic activity was observed in <i>Candida albicans</i> biofilm after treatment with the cane extract (30 mg/L) and blue grape extract (50 mg/L). The composition of cane extract was analyzed and found to be comparatively different from blue grape extract. In addition, the content of total phenolic groups in cane extract was three-times higher (12.75 g<sub>GA</sub>/L). The results showed that cane extract was more effective in preventing biofilm formation than blue grape extract and winter canes have proven to be a potential source of polyphenols for antimicrobial and antibiofilm treatment.Zdeněk KodešMaria VrublevskayaMarkéta KulišováPetr JarošMartina PaldrychováKarolína PádrováKristýna LokočováAndrea PalyzováOlga MaťátkováIrena KolouchováMDPI AGarticleresveratrolwaste product utilizationprevention of biofilm formationantioxidant activityBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2391, p 2391 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	resveratrol waste product utilization prevention of biofilm formation antioxidant activity Biology (General) QH301-705.5
spellingShingle	resveratrol waste product utilization prevention of biofilm formation antioxidant activity Biology (General) QH301-705.5 Zdeněk Kodeš Maria Vrublevskaya Markéta Kulišová Petr Jaroš Martina Paldrychová Karolína Pádrová Kristýna Lokočová Andrea Palyzová Olga Maťátková Irena Kolouchová Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm
description	<i>Vitis vinifera</i> canes are waste material of grapevine pruning and thus represent cheap source of high-value polyphenols. In view of the fact that resistance of many pathogenic microorganisms to antibiotics is a growing problem, the antimicrobial activity of plant polyphenols is studied as one of the possible approaches. We have investigated the total phenolic content, composition, antioxidant activity, and antifungal activity against <i>Candida</i> biofilm of an extract from winter canes and a commercially available extract from blue grapes. Light microscopy and confocal microscopy imaging as well as crystal violet staining were used to quantify and visualize the biofilm. We found a decrease in cell adhesion to the surface depending on the concentration of resveratrol in the cane extract. The biofilm formation was observed as metabolic activity of <i>Candida albicans</i>, <i>Candida parapsilosis</i> and <i>Candida krusei</i> biofilm cells and the minimum biofilm inhibitory concentrations were determined. The highest inhibition of metabolic activity was observed in <i>Candida albicans</i> biofilm after treatment with the cane extract (30 mg/L) and blue grape extract (50 mg/L). The composition of cane extract was analyzed and found to be comparatively different from blue grape extract. In addition, the content of total phenolic groups in cane extract was three-times higher (12.75 g<sub>GA</sub>/L). The results showed that cane extract was more effective in preventing biofilm formation than blue grape extract and winter canes have proven to be a potential source of polyphenols for antimicrobial and antibiofilm treatment.
format	article
author	Zdeněk Kodeš Maria Vrublevskaya Markéta Kulišová Petr Jaroš Martina Paldrychová Karolína Pádrová Kristýna Lokočová Andrea Palyzová Olga Maťátková Irena Kolouchová
author_facet	Zdeněk Kodeš Maria Vrublevskaya Markéta Kulišová Petr Jaroš Martina Paldrychová Karolína Pádrová Kristýna Lokočová Andrea Palyzová Olga Maťátková Irena Kolouchová
author_sort	Zdeněk Kodeš
title	Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm
title_short	Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm
title_full	Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm
title_fullStr	Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm
title_full_unstemmed	Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm
title_sort	composition and biological activity of <i>vitis vinifera</i> winter cane extract on candida biofilm
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/82e93e545ce04bc29b9b37feb10777f2
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Composition and Biological Activity of <i>Vitis vinifera</i> Winter Cane Extract on Candida Biofilm

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