Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.

<h4>Aim</h4>HIV infection is associated with distortion of T-cell homeostasis and the IL-7/IL7R axis. Progressive infection results in loss of CD127+132- and gains in CD127-132+ CD4+ and CD8+ T-cells. We investigated the correlates of loss of CD127 from the T-cell surface to understand m...

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Autores principales:	Sarah C Sasson, John J Zaunders, Nabila Seddiki, Michelle Bailey, Kristin McBride, Kersten K Koelsch, Kate M Merlin, Don E Smith, David A Cooper, Anthony D Kelleher
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82ee42fdf8e24afc9cd709cad2d3c664
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spelling	oai:doaj.org-article:82ee42fdf8e24afc9cd709cad2d3c6642021-11-18T07:28:27ZProgressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.1932-620310.1371/journal.pone.0031148https://doaj.org/article/82ee42fdf8e24afc9cd709cad2d3c6642012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22348045/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Aim</h4>HIV infection is associated with distortion of T-cell homeostasis and the IL-7/IL7R axis. Progressive infection results in loss of CD127+132- and gains in CD127-132+ CD4+ and CD8+ T-cells. We investigated the correlates of loss of CD127 from the T-cell surface to understand mechanisms underlying this homeostatic dysregulation.<h4>Methods</h4>Peripheral and cord blood mononuclear cells (PBMCs; CBMC) from healthy volunteers and PBMC from patients with HIV infection were studied. CD127+132-, CD127+132+ and CD127-132+ T-cells were phenotyped by activation, differentiation, proliferation and survival markers. Cellular HIV-DNA content and signal-joint T-cell receptor excision circles (sjTRECs) were measured.<h4>Results</h4>CD127+132- T-cells were enriched for naïve cells while CD127-132+ T-cells were enriched for activated/terminally differentiated T-cells in CD4+ and CD8+ subsets in health and HIV infection. HIV was associated with increased proportions of activated/terminally differentiated CD127-132+ T-cells. In contrast to CD127+132- T-cells, CD127-132+ T-cells were Ki-67+Bcl-2(low) and contained increased levels of HIV-DNA. Naïve CD127+132- T-cells contained a higher proportion of sjTRECs.<h4>Conclusion</h4>The loss of CD127 from the T-cell surface in HIV infection is driven by activation of CD127+132- recent thymic emigrants into CD127-132+ activated/terminally differentiated cells. This process likely results in an irreversible loss of CD127 and permanent distortion of T-cell homeostasis.Sarah C SassonJohn J ZaundersNabila SeddikiMichelle BaileyKristin McBrideKersten K KoelschKate M MerlinDon E SmithDavid A CooperAnthony D KelleherPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31148 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah C Sasson John J Zaunders Nabila Seddiki Michelle Bailey Kristin McBride Kersten K Koelsch Kate M Merlin Don E Smith David A Cooper Anthony D Kelleher Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.
description	<h4>Aim</h4>HIV infection is associated with distortion of T-cell homeostasis and the IL-7/IL7R axis. Progressive infection results in loss of CD127+132- and gains in CD127-132+ CD4+ and CD8+ T-cells. We investigated the correlates of loss of CD127 from the T-cell surface to understand mechanisms underlying this homeostatic dysregulation.<h4>Methods</h4>Peripheral and cord blood mononuclear cells (PBMCs; CBMC) from healthy volunteers and PBMC from patients with HIV infection were studied. CD127+132-, CD127+132+ and CD127-132+ T-cells were phenotyped by activation, differentiation, proliferation and survival markers. Cellular HIV-DNA content and signal-joint T-cell receptor excision circles (sjTRECs) were measured.<h4>Results</h4>CD127+132- T-cells were enriched for naïve cells while CD127-132+ T-cells were enriched for activated/terminally differentiated T-cells in CD4+ and CD8+ subsets in health and HIV infection. HIV was associated with increased proportions of activated/terminally differentiated CD127-132+ T-cells. In contrast to CD127+132- T-cells, CD127-132+ T-cells were Ki-67+Bcl-2(low) and contained increased levels of HIV-DNA. Naïve CD127+132- T-cells contained a higher proportion of sjTRECs.<h4>Conclusion</h4>The loss of CD127 from the T-cell surface in HIV infection is driven by activation of CD127+132- recent thymic emigrants into CD127-132+ activated/terminally differentiated cells. This process likely results in an irreversible loss of CD127 and permanent distortion of T-cell homeostasis.
format	article
author	Sarah C Sasson John J Zaunders Nabila Seddiki Michelle Bailey Kristin McBride Kersten K Koelsch Kate M Merlin Don E Smith David A Cooper Anthony D Kelleher
author_facet	Sarah C Sasson John J Zaunders Nabila Seddiki Michelle Bailey Kristin McBride Kersten K Koelsch Kate M Merlin Don E Smith David A Cooper Anthony D Kelleher
author_sort	Sarah C Sasson
title	Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.
title_short	Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.
title_full	Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.
title_fullStr	Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.
title_full_unstemmed	Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.
title_sort	progressive activation of cd127+132- recent thymic emigrants into terminally differentiated cd127-132+ t-cells in hiv-1 infection.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/82ee42fdf8e24afc9cd709cad2d3c664
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Progressive activation of CD127+132- recent thymic emigrants into terminally differentiated CD127-132+ T-cells in HIV-1 infection.

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