Effects of growth rate and promoter activity on single-cell protein expression

Abstract Protein expression in a single cell depends on its global physiological state. Moreover, genetically-identical cells exhibit variability (noise) in protein expression, arising from the stochastic nature of biochemical processes, cell growth and division. While it is well understood how cell...

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Auteurs principaux:	Niclas Nordholt, Johan van Heerden, Remco Kort, Frank J. Bruggeman
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2017
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/82ef0677080943fda576e0581cd769a4
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id	oai:doaj.org-article:82ef0677080943fda576e0581cd769a4
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spelling	oai:doaj.org-article:82ef0677080943fda576e0581cd769a42021-12-02T15:05:51ZEffects of growth rate and promoter activity on single-cell protein expression10.1038/s41598-017-05871-32045-2322https://doaj.org/article/82ef0677080943fda576e0581cd769a42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05871-3https://doaj.org/toc/2045-2322Abstract Protein expression in a single cell depends on its global physiological state. Moreover, genetically-identical cells exhibit variability (noise) in protein expression, arising from the stochastic nature of biochemical processes, cell growth and division. While it is well understood how cellular growth rate influences mean protein expression, little is known about the relationship between growth rate and noise in protein expression. Here we quantify this relationship in Bacillus subtilis by a novel combination of experiments and theory. We measure the effects of promoter activity and growth rate on the expression of a fluorescent protein in single cells. We disentangle the observed protein expression noise into protein-specific and systemic contributions, using theory and variance decomposition. We find that noise in protein expression depends solely on mean expression levels, regardless of whether expression is set by promoter activity or growth rate, and that noise increases linearly with growth rate. Our results can aid studies of (synthetic) gene circuits of single cells and their condition dependence.Niclas NordholtJohan van HeerdenRemco KortFrank J. BruggemanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Niclas Nordholt Johan van Heerden Remco Kort Frank J. Bruggeman Effects of growth rate and promoter activity on single-cell protein expression
description	Abstract Protein expression in a single cell depends on its global physiological state. Moreover, genetically-identical cells exhibit variability (noise) in protein expression, arising from the stochastic nature of biochemical processes, cell growth and division. While it is well understood how cellular growth rate influences mean protein expression, little is known about the relationship between growth rate and noise in protein expression. Here we quantify this relationship in Bacillus subtilis by a novel combination of experiments and theory. We measure the effects of promoter activity and growth rate on the expression of a fluorescent protein in single cells. We disentangle the observed protein expression noise into protein-specific and systemic contributions, using theory and variance decomposition. We find that noise in protein expression depends solely on mean expression levels, regardless of whether expression is set by promoter activity or growth rate, and that noise increases linearly with growth rate. Our results can aid studies of (synthetic) gene circuits of single cells and their condition dependence.
format	article
author	Niclas Nordholt Johan van Heerden Remco Kort Frank J. Bruggeman
author_facet	Niclas Nordholt Johan van Heerden Remco Kort Frank J. Bruggeman
author_sort	Niclas Nordholt
title	Effects of growth rate and promoter activity on single-cell protein expression
title_short	Effects of growth rate and promoter activity on single-cell protein expression
title_full	Effects of growth rate and promoter activity on single-cell protein expression
title_fullStr	Effects of growth rate and promoter activity on single-cell protein expression
title_full_unstemmed	Effects of growth rate and promoter activity on single-cell protein expression
title_sort	effects of growth rate and promoter activity on single-cell protein expression
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/82ef0677080943fda576e0581cd769a4
work_keys_str_mv	AT niclasnordholt effectsofgrowthrateandpromoteractivityonsinglecellproteinexpression AT johanvanheerden effectsofgrowthrateandpromoteractivityonsinglecellproteinexpression AT remcokort effectsofgrowthrateandpromoteractivityonsinglecellproteinexpression AT frankjbruggeman effectsofgrowthrateandpromoteractivityonsinglecellproteinexpression
_version_	1718388653472874496

Effects of growth rate and promoter activity on single-cell protein expression

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