Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues

Abstract We evaluated the duloxetine DNA damaging capacity utilizing the comet assay applied to mouse brain and liver cells, as well as its DNA, lipid, protein, and nitric oxide oxidative potential in the same cells. A kinetic time/dose strategy showed the effect of 2, 20, and 200 mg/kg of the drug...

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Autores principales:	Isela Álvarez-González, Scarlett Camacho-Cantera, Patricia Gómez-González, Michael J. Rendón Barrón, José A. Morales-González, Eduardo O. Madrigal-Santillán, Rogelio Paniagua-Pérez, Eduardo Madrigal-Bujaidar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/82f7095f37b346aea7251153626e1055
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spelling	oai:doaj.org-article:82f7095f37b346aea7251153626e10552021-12-02T13:24:25ZGenotoxic and oxidative effect of duloxetine on mouse brain and liver tissues10.1038/s41598-021-86366-02045-2322https://doaj.org/article/82f7095f37b346aea7251153626e10552021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86366-0https://doaj.org/toc/2045-2322Abstract We evaluated the duloxetine DNA damaging capacity utilizing the comet assay applied to mouse brain and liver cells, as well as its DNA, lipid, protein, and nitric oxide oxidative potential in the same cells. A kinetic time/dose strategy showed the effect of 2, 20, and 200 mg/kg of the drug administered intraperitoneally once in comparison with a control and a methyl methanesulfonate group. Each parameter was evaluated at 3, 9, 15, and 21 h postadministration in five mice per group, except for the DNA oxidation that was examined only at 9 h postadministration. Results showed a significant DNA damage mainly at 9 h postexposure in both organs. In the brain, with 20 and 200 mg/kg we found 50 and 80% increase over the control group (p ≤ 0.05), in the liver, the increase of 2, 20, and 200 mg/kg of duloxetine was 50, 80, and 135% in comparison with the control level (p ≤ 0.05). DNA, lipid, protein and nitric oxide oxidation increase was also observed in both organs. Our data established the DNA damaging capacity of duloxetine even with a dose from the therapeutic range (2 mg/kg), and suggest that this effect can be related with its oxidative potential.Isela Álvarez-GonzálezScarlett Camacho-CanteraPatricia Gómez-GonzálezMichael J. Rendón BarrónJosé A. Morales-GonzálezEduardo O. Madrigal-SantillánRogelio Paniagua-PérezEduardo Madrigal-BujaidarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isela Álvarez-González Scarlett Camacho-Cantera Patricia Gómez-González Michael J. Rendón Barrón José A. Morales-González Eduardo O. Madrigal-Santillán Rogelio Paniagua-Pérez Eduardo Madrigal-Bujaidar Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
description	Abstract We evaluated the duloxetine DNA damaging capacity utilizing the comet assay applied to mouse brain and liver cells, as well as its DNA, lipid, protein, and nitric oxide oxidative potential in the same cells. A kinetic time/dose strategy showed the effect of 2, 20, and 200 mg/kg of the drug administered intraperitoneally once in comparison with a control and a methyl methanesulfonate group. Each parameter was evaluated at 3, 9, 15, and 21 h postadministration in five mice per group, except for the DNA oxidation that was examined only at 9 h postadministration. Results showed a significant DNA damage mainly at 9 h postexposure in both organs. In the brain, with 20 and 200 mg/kg we found 50 and 80% increase over the control group (p ≤ 0.05), in the liver, the increase of 2, 20, and 200 mg/kg of duloxetine was 50, 80, and 135% in comparison with the control level (p ≤ 0.05). DNA, lipid, protein and nitric oxide oxidation increase was also observed in both organs. Our data established the DNA damaging capacity of duloxetine even with a dose from the therapeutic range (2 mg/kg), and suggest that this effect can be related with its oxidative potential.
format	article
author	Isela Álvarez-González Scarlett Camacho-Cantera Patricia Gómez-González Michael J. Rendón Barrón José A. Morales-González Eduardo O. Madrigal-Santillán Rogelio Paniagua-Pérez Eduardo Madrigal-Bujaidar
author_facet	Isela Álvarez-González Scarlett Camacho-Cantera Patricia Gómez-González Michael J. Rendón Barrón José A. Morales-González Eduardo O. Madrigal-Santillán Rogelio Paniagua-Pérez Eduardo Madrigal-Bujaidar
author_sort	Isela Álvarez-González
title	Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
title_short	Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
title_full	Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
title_fullStr	Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
title_full_unstemmed	Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
title_sort	genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/82f7095f37b346aea7251153626e1055
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Genotoxic and oxidative effect of duloxetine on mouse brain and liver tissues

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