Pak1 kinase controls cell shape through ribonucleoprotein granules

Fission yeast cells maintain a rod shape due to conserved signaling pathways that organize the cytoskeleton for polarized growth. We discovered a mechanism linking the conserved protein kinase Pak1 with cell shape through the RNA-binding protein Sts5. Pak1 (also called Shk1 and Orb2) prevents Sts5 a...

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Autores principales:	Joseph O Magliozzi, James B Moseley
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Pak1 Sts5 Orb6 polarity P body pombe Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/82fcbf066efd4e438e2007e9127fd757
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spelling	oai:doaj.org-article:82fcbf066efd4e438e2007e9127fd7572021-11-14T15:26:29ZPak1 kinase controls cell shape through ribonucleoprotein granules10.7554/eLife.676482050-084Xe67648https://doaj.org/article/82fcbf066efd4e438e2007e9127fd7572021-07-01T00:00:00Zhttps://elifesciences.org/articles/67648https://doaj.org/toc/2050-084XFission yeast cells maintain a rod shape due to conserved signaling pathways that organize the cytoskeleton for polarized growth. We discovered a mechanism linking the conserved protein kinase Pak1 with cell shape through the RNA-binding protein Sts5. Pak1 (also called Shk1 and Orb2) prevents Sts5 association with P bodies by directly phosphorylating its intrinsically disordered region (IDR). Pak1 and the cell polarity kinase Orb6 both phosphorylate the Sts5 IDR but at distinct residues. Mutations preventing phosphorylation in the Sts5 IDR cause increased P body formation and defects in cell shape and polarity. Unexpectedly, when cells encounter glucose starvation, PKA signaling triggers Pak1 recruitment to stress granules with Sts5. Through retargeting experiments, we reveal that Pak1 localizes to stress granules to promote rapid dissolution of Sts5 upon glucose addition. Our work reveals a new role for Pak1 in regulating cell shape through ribonucleoprotein granules during normal and stressed growth conditions.Joseph O MagliozziJames B MoseleyeLife Sciences Publications LtdarticlePak1Sts5Orb6polarityP bodypombeMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pak1 Sts5 Orb6 polarity P body pombe Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Pak1 Sts5 Orb6 polarity P body pombe Medicine R Science Q Biology (General) QH301-705.5 Joseph O Magliozzi James B Moseley Pak1 kinase controls cell shape through ribonucleoprotein granules
description	Fission yeast cells maintain a rod shape due to conserved signaling pathways that organize the cytoskeleton for polarized growth. We discovered a mechanism linking the conserved protein kinase Pak1 with cell shape through the RNA-binding protein Sts5. Pak1 (also called Shk1 and Orb2) prevents Sts5 association with P bodies by directly phosphorylating its intrinsically disordered region (IDR). Pak1 and the cell polarity kinase Orb6 both phosphorylate the Sts5 IDR but at distinct residues. Mutations preventing phosphorylation in the Sts5 IDR cause increased P body formation and defects in cell shape and polarity. Unexpectedly, when cells encounter glucose starvation, PKA signaling triggers Pak1 recruitment to stress granules with Sts5. Through retargeting experiments, we reveal that Pak1 localizes to stress granules to promote rapid dissolution of Sts5 upon glucose addition. Our work reveals a new role for Pak1 in regulating cell shape through ribonucleoprotein granules during normal and stressed growth conditions.
format	article
author	Joseph O Magliozzi James B Moseley
author_facet	Joseph O Magliozzi James B Moseley
author_sort	Joseph O Magliozzi
title	Pak1 kinase controls cell shape through ribonucleoprotein granules
title_short	Pak1 kinase controls cell shape through ribonucleoprotein granules
title_full	Pak1 kinase controls cell shape through ribonucleoprotein granules
title_fullStr	Pak1 kinase controls cell shape through ribonucleoprotein granules
title_full_unstemmed	Pak1 kinase controls cell shape through ribonucleoprotein granules
title_sort	pak1 kinase controls cell shape through ribonucleoprotein granules
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/82fcbf066efd4e438e2007e9127fd757
work_keys_str_mv	AT josephomagliozzi pak1kinasecontrolscellshapethroughribonucleoproteingranules AT jamesbmoseley pak1kinasecontrolscellshapethroughribonucleoproteingranules
_version_	1718429058720595968

Pak1 kinase controls cell shape through ribonucleoprotein granules

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