Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.

The objective of this work is to investigate the relationship between endo-β-mannanase and leaf abscission, and response to wounding in soybean (Glycine max). An endo-β-mannanase gene GmMAN1 was cloned from the abscission zone in petiole explants, and was heterologously expressed in E. coli. Polyclo...

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Autores principales:	Min Yan, Yifan Zhang, Wenjuan Guo, Xiaofeng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8305ee7cfdf842db84947e341c406e03
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spelling	oai:doaj.org-article:8305ee7cfdf842db84947e341c406e032021-11-18T08:08:22ZSoybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.1932-620310.1371/journal.pone.0049197https://doaj.org/article/8305ee7cfdf842db84947e341c406e032012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23173047/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The objective of this work is to investigate the relationship between endo-β-mannanase and leaf abscission, and response to wounding in soybean (Glycine max). An endo-β-mannanase gene GmMAN1 was cloned from the abscission zone in petiole explants, and was heterologously expressed in E. coli. Polyclonal antibodies were raised against the fusion protein. The increases in activity, isoform numbers, and amounts of transcripts and proteins of GmMAN1 were found not only in the abscission zone but also in the non-abscission zone during petiole abscission in the explants, but not in these two tissues during leaf abscission artificially induced by ethephon treatment in the intact plants. The changes in endo-β-mannanase expression patterns in these two tissues were probably induced by the inherent mechanical wounding during the preparation of explants. When soybean plants were wounded by removing half of the leaf blade of the first pair of true leaves, the transcripts and proteins of GmMAN1 were induced in the leaves and stem, leading to the increases in enzyme activity and isoform numbers in them. It is concluded that the soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.Min YanYifan ZhangWenjuan GuoXiaofeng WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49197 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Min Yan Yifan Zhang Wenjuan Guo Xiaofeng Wang Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
description	The objective of this work is to investigate the relationship between endo-β-mannanase and leaf abscission, and response to wounding in soybean (Glycine max). An endo-β-mannanase gene GmMAN1 was cloned from the abscission zone in petiole explants, and was heterologously expressed in E. coli. Polyclonal antibodies were raised against the fusion protein. The increases in activity, isoform numbers, and amounts of transcripts and proteins of GmMAN1 were found not only in the abscission zone but also in the non-abscission zone during petiole abscission in the explants, but not in these two tissues during leaf abscission artificially induced by ethephon treatment in the intact plants. The changes in endo-β-mannanase expression patterns in these two tissues were probably induced by the inherent mechanical wounding during the preparation of explants. When soybean plants were wounded by removing half of the leaf blade of the first pair of true leaves, the transcripts and proteins of GmMAN1 were induced in the leaves and stem, leading to the increases in enzyme activity and isoform numbers in them. It is concluded that the soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
format	article
author	Min Yan Yifan Zhang Wenjuan Guo Xiaofeng Wang
author_facet	Min Yan Yifan Zhang Wenjuan Guo Xiaofeng Wang
author_sort	Min Yan
title	Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
title_short	Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
title_full	Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
title_fullStr	Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
title_full_unstemmed	Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.
title_sort	soybean endo-β-mannanase gmman1 is not associated with leaf abscission, but might be involved in the response to wounding.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8305ee7cfdf842db84947e341c406e03
work_keys_str_mv	AT minyan soybeanendobmannanasegmman1isnotassociatedwithleafabscissionbutmightbeinvolvedintheresponsetowounding AT yifanzhang soybeanendobmannanasegmman1isnotassociatedwithleafabscissionbutmightbeinvolvedintheresponsetowounding AT wenjuanguo soybeanendobmannanasegmman1isnotassociatedwithleafabscissionbutmightbeinvolvedintheresponsetowounding AT xiaofengwang soybeanendobmannanasegmman1isnotassociatedwithleafabscissionbutmightbeinvolvedintheresponsetowounding
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Soybean endo-β-mannanase GmMAN1 is not associated with leaf abscission, but might be involved in the response to wounding.

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