Impact of diesel and biodiesel contamination on soil microbial community activity and structure

Abstract Soil contamination as a result of oil spills is a serious issue due to the global demand for diesel fuel. As an alternative to diesel, biodiesel has been introduced based on its high degradability rates and potential for reducing of greenhouse gases emissions. This study assessed the impact...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eduardo K. Mitter, James J. Germida, J. Renato de Freitas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/830ba78b953a42ffaba3f7c485f36eb9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:830ba78b953a42ffaba3f7c485f36eb9
record_format	dspace
spelling	oai:doaj.org-article:830ba78b953a42ffaba3f7c485f36eb92021-12-02T16:53:01ZImpact of diesel and biodiesel contamination on soil microbial community activity and structure10.1038/s41598-021-89637-y2045-2322https://doaj.org/article/830ba78b953a42ffaba3f7c485f36eb92021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89637-yhttps://doaj.org/toc/2045-2322Abstract Soil contamination as a result of oil spills is a serious issue due to the global demand for diesel fuel. As an alternative to diesel, biodiesel has been introduced based on its high degradability rates and potential for reducing of greenhouse gases emissions. This study assessed the impacts diesel and biodiesel contamination on soil microbial community activity and structure. Our results suggest higher microbial activity in biodiesel contaminated soils and analysis of PLFA profiles confirmed shifts in microbial community structure in response to contamination. High-throughput 16S rRNA amplicon sequencing also revealed a lower bacterial richness and diversity in contaminated soils when compared to control samples, supporting evidence of the detrimental effects of hydrocarbons on soil microbiota. Control samples comprised mostly of Actinobacteria, whereas Proteobacteria were predominantly observed in diesel and biodiesel contaminated soils. At genus level, diesel and biodiesel amendments highly selected for Rhodococcus and Pseudomonas spp., respectively. Moreover, predicted functional profiles based on hydrocarbon-degrading enzymes revealed significant differences between contaminated soils mostly due to the chemical composition of diesel and biodiesel fuel. Here, we also identified that Burkholderiaceae, Novosphingobium, Anaeromyxobacter, Pseudomonas and Rhodococcus were the main bacterial taxa contributing to these enzymes. Together, this study supports the evidence of diesel/biodiesel adverse effects in soil microbial community structure and highlights microbial taxa that could be further investigated for their biodegradation potential.Eduardo K. MitterJames J. GermidaJ. Renato de FreitasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eduardo K. Mitter James J. Germida J. Renato de Freitas Impact of diesel and biodiesel contamination on soil microbial community activity and structure
description	Abstract Soil contamination as a result of oil spills is a serious issue due to the global demand for diesel fuel. As an alternative to diesel, biodiesel has been introduced based on its high degradability rates and potential for reducing of greenhouse gases emissions. This study assessed the impacts diesel and biodiesel contamination on soil microbial community activity and structure. Our results suggest higher microbial activity in biodiesel contaminated soils and analysis of PLFA profiles confirmed shifts in microbial community structure in response to contamination. High-throughput 16S rRNA amplicon sequencing also revealed a lower bacterial richness and diversity in contaminated soils when compared to control samples, supporting evidence of the detrimental effects of hydrocarbons on soil microbiota. Control samples comprised mostly of Actinobacteria, whereas Proteobacteria were predominantly observed in diesel and biodiesel contaminated soils. At genus level, diesel and biodiesel amendments highly selected for Rhodococcus and Pseudomonas spp., respectively. Moreover, predicted functional profiles based on hydrocarbon-degrading enzymes revealed significant differences between contaminated soils mostly due to the chemical composition of diesel and biodiesel fuel. Here, we also identified that Burkholderiaceae, Novosphingobium, Anaeromyxobacter, Pseudomonas and Rhodococcus were the main bacterial taxa contributing to these enzymes. Together, this study supports the evidence of diesel/biodiesel adverse effects in soil microbial community structure and highlights microbial taxa that could be further investigated for their biodegradation potential.
format	article
author	Eduardo K. Mitter James J. Germida J. Renato de Freitas
author_facet	Eduardo K. Mitter James J. Germida J. Renato de Freitas
author_sort	Eduardo K. Mitter
title	Impact of diesel and biodiesel contamination on soil microbial community activity and structure
title_short	Impact of diesel and biodiesel contamination on soil microbial community activity and structure
title_full	Impact of diesel and biodiesel contamination on soil microbial community activity and structure
title_fullStr	Impact of diesel and biodiesel contamination on soil microbial community activity and structure
title_full_unstemmed	Impact of diesel and biodiesel contamination on soil microbial community activity and structure
title_sort	impact of diesel and biodiesel contamination on soil microbial community activity and structure
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/830ba78b953a42ffaba3f7c485f36eb9
work_keys_str_mv	AT eduardokmitter impactofdieselandbiodieselcontaminationonsoilmicrobialcommunityactivityandstructure AT jamesjgermida impactofdieselandbiodieselcontaminationonsoilmicrobialcommunityactivityandstructure AT jrenatodefreitas impactofdieselandbiodieselcontaminationonsoilmicrobialcommunityactivityandstructure
_version_	1718382870379102208

Impact of diesel and biodiesel contamination on soil microbial community activity and structure

Ejemplares similares