A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants

Quantitative analysis of how DNA sequence determines transcription and translation regulation is of interest to systems and synthetic biologists. Here the authors present ELM-seq, which uses Dam activity as reporter for high-throughput analysis of promoter and 5’-UTR regions.

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Autores principales:	Eva Yus, Jae-Seong Yang, Adrià Sogues, Luis Serrano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/831799865ba64682af69f894155832d0
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spelling	oai:doaj.org-article:831799865ba64682af69f894155832d02021-12-02T15:38:39ZA reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants10.1038/s41467-017-00239-72041-1723https://doaj.org/article/831799865ba64682af69f894155832d02017-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00239-7https://doaj.org/toc/2041-1723Quantitative analysis of how DNA sequence determines transcription and translation regulation is of interest to systems and synthetic biologists. Here the authors present ELM-seq, which uses Dam activity as reporter for high-throughput analysis of promoter and 5’-UTR regions.Eva YusJae-Seong YangAdrià SoguesLuis SerranoNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Eva Yus Jae-Seong Yang Adrià Sogues Luis Serrano A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
description	Quantitative analysis of how DNA sequence determines transcription and translation regulation is of interest to systems and synthetic biologists. Here the authors present ELM-seq, which uses Dam activity as reporter for high-throughput analysis of promoter and 5’-UTR regions.
format	article
author	Eva Yus Jae-Seong Yang Adrià Sogues Luis Serrano
author_facet	Eva Yus Jae-Seong Yang Adrià Sogues Luis Serrano
author_sort	Eva Yus
title	A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
title_short	A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
title_full	A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
title_fullStr	A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
title_full_unstemmed	A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
title_sort	reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/831799865ba64682af69f894155832d0
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A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants

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