A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants

A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants

Quantitative analysis of how DNA sequence determines transcription and translation regulation is of interest to systems and synthetic biologists. Here the authors present ELM-seq, which uses Dam activity as reporter for high-throughput analysis of promoter and 5’-UTR regions.

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Detalles Bibliográficos
Autores principales: Eva Yus, Jae-Seong Yang, Adrià Sogues, Luis Serrano
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Science
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Acceso en línea:https://doaj.org/article/831799865ba64682af69f894155832d0
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