Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis

RNA polymerase III (RNAPIII) contains 17 subunits forming 4 functional domains that control the different stages of RNAPIII transcription and are dedicated to the synthesis of small RNAs such as 5S rRNA and tRNAs. Here, we identified 23 genes encoding these subunits in Arabidopsis (<i>Arabidop...

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Autores principales:	Hailiang Zhao, Yao Qin, Ziyi Xiao, Kun Liang, Dianming Gong, Qin Sun, Fazhan Qiu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	RNA polymerase III subunits 5S rRNA tRNA seed development Arabidopsis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/832a2eecd1a0418fafef1eda498f79ea
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spelling	oai:doaj.org-article:832a2eecd1a0418fafef1eda498f79ea2021-11-11T16:48:14ZKnockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis10.3390/ijms2221113141422-00671661-6596https://doaj.org/article/832a2eecd1a0418fafef1eda498f79ea2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11314https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067RNA polymerase III (RNAPIII) contains 17 subunits forming 4 functional domains that control the different stages of RNAPIII transcription and are dedicated to the synthesis of small RNAs such as 5S rRNA and tRNAs. Here, we identified 23 genes encoding these subunits in Arabidopsis (<i>Arabidopsis thaliana</i>) and further analyzed 5 subunits (NRPC2, NRPC3, NRPC8, NRPABC1, and NRPABC2) encoded by 6 genes with different expression patterns and belonging to different sub-complexes. The knockdown of these genes repressed the expression of 5S rRNA and tRNAs, causing seed developmental arrest at different stages. Among these knockdown mutants, RNA-seq analysis revealed 821 common differentially expressed genes (DEGs), significantly enriched in response to stress, abscisic acid, cytokinins, and the jasmonic acid signaling pathway. Weighted gene co-expression network analysis (WGCNA) revealed several hub genes involved in embryo development, carbohydrate metabolic and lipid metabolic processes. We identified numerous unique DEGs between the mutants belonging to pathways, including cell proliferation, ribosome biogenesis, cell death, and tRNA metabolic processes. Thus, <i>NRPC2</i>, <i>NRPC3</i>, <i>NRPC8</i>, <i>NRPABC1</i>, and <i>NRPABC2</i> control seed development in Arabidopsis by influencing RNAPIII activity and, thus, hormone signaling. Reduced expression of these subunit genes causes an insufficient accumulation of the total RNAPIII, leading to the phenotypes observed following the genetic knockdown of these subunits.Hailiang ZhaoYao QinZiyi XiaoKun LiangDianming GongQin SunFazhan QiuMDPI AGarticleRNA polymerase III subunits5S rRNAtRNAseed developmentArabidopsisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11314, p 11314 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA polymerase III subunits 5S rRNA tRNA seed development Arabidopsis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	RNA polymerase III subunits 5S rRNA tRNA seed development Arabidopsis Biology (General) QH301-705.5 Chemistry QD1-999 Hailiang Zhao Yao Qin Ziyi Xiao Kun Liang Dianming Gong Qin Sun Fazhan Qiu Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis
description	RNA polymerase III (RNAPIII) contains 17 subunits forming 4 functional domains that control the different stages of RNAPIII transcription and are dedicated to the synthesis of small RNAs such as 5S rRNA and tRNAs. Here, we identified 23 genes encoding these subunits in Arabidopsis (<i>Arabidopsis thaliana</i>) and further analyzed 5 subunits (NRPC2, NRPC3, NRPC8, NRPABC1, and NRPABC2) encoded by 6 genes with different expression patterns and belonging to different sub-complexes. The knockdown of these genes repressed the expression of 5S rRNA and tRNAs, causing seed developmental arrest at different stages. Among these knockdown mutants, RNA-seq analysis revealed 821 common differentially expressed genes (DEGs), significantly enriched in response to stress, abscisic acid, cytokinins, and the jasmonic acid signaling pathway. Weighted gene co-expression network analysis (WGCNA) revealed several hub genes involved in embryo development, carbohydrate metabolic and lipid metabolic processes. We identified numerous unique DEGs between the mutants belonging to pathways, including cell proliferation, ribosome biogenesis, cell death, and tRNA metabolic processes. Thus, <i>NRPC2</i>, <i>NRPC3</i>, <i>NRPC8</i>, <i>NRPABC1</i>, and <i>NRPABC2</i> control seed development in Arabidopsis by influencing RNAPIII activity and, thus, hormone signaling. Reduced expression of these subunit genes causes an insufficient accumulation of the total RNAPIII, leading to the phenotypes observed following the genetic knockdown of these subunits.
format	article
author	Hailiang Zhao Yao Qin Ziyi Xiao Kun Liang Dianming Gong Qin Sun Fazhan Qiu
author_facet	Hailiang Zhao Yao Qin Ziyi Xiao Kun Liang Dianming Gong Qin Sun Fazhan Qiu
author_sort	Hailiang Zhao
title	Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis
title_short	Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis
title_full	Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis
title_fullStr	Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis
title_full_unstemmed	Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis
title_sort	knockdown nrpc2, 3, 8, nrpabc1 and nrpabc2 affects rnapiii activity and disrupts seed development in arabidopsis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/832a2eecd1a0418fafef1eda498f79ea
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Knockdown NRPC2, 3, 8, NRPABC1 and NRPABC2 Affects RNAPIII Activity and Disrupts Seed Development in Arabidopsis

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