Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging

Abstract Washed textiles can remain malodorous and dingy due to the recalcitrance of soils. Recent work has found that ‘invisible’ soils such as microbial extracellular DNA (eDNA) play a key role in the adhesion of extracellular polymeric substances that form matrixes contributing to these undesirab...

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Autores principales:	Hamish C. L. Yau, Adam K. Malekpour, Nazarmohammad G. Momin, Ana L. Morales-García, William G. T. Willats, Neil J. Lant, Catherine Y. Jones
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/832a6ce7ac724667ad4a7d6a9e38bec3
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spelling	oai:doaj.org-article:832a6ce7ac724667ad4a7d6a9e38bec32021-11-08T10:49:16ZRemoval of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging10.1038/s41598-021-98939-02045-2322https://doaj.org/article/832a6ce7ac724667ad4a7d6a9e38bec32021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98939-0https://doaj.org/toc/2045-2322Abstract Washed textiles can remain malodorous and dingy due to the recalcitrance of soils. Recent work has found that ‘invisible’ soils such as microbial extracellular DNA (eDNA) play a key role in the adhesion of extracellular polymeric substances that form matrixes contributing to these undesirable characteristics. Here we report the application of an immunostaining method to illustrate the cleaning mechanism of a nuclease (DNase I) acting upon eDNA. Extending previous work that established a key role for eDNA in anchoring these soil matrixes, this work provides new insights into the presence and effective removal of eDNA deposited on fabrics using high-resolution in-situ imaging. Using a monoclonal antibody specific to Z-DNA, we showed that when fabrics are washed with DNase I, the incidence of microbial eDNA is reduced. As well as a quantitative reduction in microbial eDNA, the deep cleaning benefits of this enzyme are shown using confocal microscopy and imaging analysis of T-shirt fibers. To the best of our knowledge, this is the first time the use of a molecular probe has been leveraged for fabric and homecare-related R&D to visualize eDNA and evaluate its removal from textiles by a new-to-laundry DNase enzyme. The approaches described in the current work also have scope for re-application to identify further cleaning technology.Hamish C. L. YauAdam K. MalekpourNazarmohammad G. MominAna L. Morales-GarcíaWilliam G. T. WillatsNeil J. LantCatherine Y. JonesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamish C. L. Yau Adam K. Malekpour Nazarmohammad G. Momin Ana L. Morales-García William G. T. Willats Neil J. Lant Catherine Y. Jones Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging
description	Abstract Washed textiles can remain malodorous and dingy due to the recalcitrance of soils. Recent work has found that ‘invisible’ soils such as microbial extracellular DNA (eDNA) play a key role in the adhesion of extracellular polymeric substances that form matrixes contributing to these undesirable characteristics. Here we report the application of an immunostaining method to illustrate the cleaning mechanism of a nuclease (DNase I) acting upon eDNA. Extending previous work that established a key role for eDNA in anchoring these soil matrixes, this work provides new insights into the presence and effective removal of eDNA deposited on fabrics using high-resolution in-situ imaging. Using a monoclonal antibody specific to Z-DNA, we showed that when fabrics are washed with DNase I, the incidence of microbial eDNA is reduced. As well as a quantitative reduction in microbial eDNA, the deep cleaning benefits of this enzyme are shown using confocal microscopy and imaging analysis of T-shirt fibers. To the best of our knowledge, this is the first time the use of a molecular probe has been leveraged for fabric and homecare-related R&D to visualize eDNA and evaluate its removal from textiles by a new-to-laundry DNase enzyme. The approaches described in the current work also have scope for re-application to identify further cleaning technology.
format	article
author	Hamish C. L. Yau Adam K. Malekpour Nazarmohammad G. Momin Ana L. Morales-García William G. T. Willats Neil J. Lant Catherine Y. Jones
author_facet	Hamish C. L. Yau Adam K. Malekpour Nazarmohammad G. Momin Ana L. Morales-García William G. T. Willats Neil J. Lant Catherine Y. Jones
author_sort	Hamish C. L. Yau
title	Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging
title_short	Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging
title_full	Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging
title_fullStr	Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging
title_full_unstemmed	Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging
title_sort	removal of edna from fabrics using a novel laundry dnase revealed using high-resolution imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/832a6ce7ac724667ad4a7d6a9e38bec3
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Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging

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