Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).

Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Sam...

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Autores principales:	Jean-Claude Caissard, Thomas Olivier, Claire Delbecque, Sabine Palle, Pierre-Philippe Garry, Arthur Audran, Nadine Valot, Sandrine Moja, Florence Nicolé, Jean-Louis Magnard, Sylvain Legrand, Sylvie Baudino, Frédéric Jullien
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/832bdce77de34b74855fab383146651d
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spelling	oai:doaj.org-article:832bdce77de34b74855fab383146651d2021-11-18T08:10:56ZExtracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).1932-620310.1371/journal.pone.0048253https://doaj.org/article/832bdce77de34b74855fab383146651d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23133579/?tool=EBIhttps://doaj.org/toc/1932-6203Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.Jean-Claude CaissardThomas OlivierClaire DelbecqueSabine PallePierre-Philippe GarryArthur AudranNadine ValotSandrine MojaFlorence NicoléJean-Louis MagnardSylvain LegrandSylvie BaudinoFrédéric JullienPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e48253 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jean-Claude Caissard Thomas Olivier Claire Delbecque Sabine Palle Pierre-Philippe Garry Arthur Audran Nadine Valot Sandrine Moja Florence Nicolé Jean-Louis Magnard Sylvain Legrand Sylvie Baudino Frédéric Jullien Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
description	Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.
format	article
author	Jean-Claude Caissard Thomas Olivier Claire Delbecque Sabine Palle Pierre-Philippe Garry Arthur Audran Nadine Valot Sandrine Moja Florence Nicolé Jean-Louis Magnard Sylvain Legrand Sylvie Baudino Frédéric Jullien
author_facet	Jean-Claude Caissard Thomas Olivier Claire Delbecque Sabine Palle Pierre-Philippe Garry Arthur Audran Nadine Valot Sandrine Moja Florence Nicolé Jean-Louis Magnard Sylvain Legrand Sylvie Baudino Frédéric Jullien
author_sort	Jean-Claude Caissard
title	Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
title_short	Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
title_full	Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
title_fullStr	Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
title_full_unstemmed	Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
title_sort	extracellular localization of the diterpene sclareol in clary sage (salvia sclarea l., lamiaceae).
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/832bdce77de34b74855fab383146651d
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Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).

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