Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines

Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the synthesis of Au nanoparticles by pectin (PEC) as a natural reducing and stabilizing agent without using any toxic and harmful reagent under ultrasonic condition. The biosynthesized Au NPs@PEC we...

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Autores principales:	Dongxiao Wang, Jin Pu, Yuebin Liao, Jinhua Liu, Gen Hu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Pectin Gold nanoparticles DNA fragmentation Apoptosis Antioxidant Acute lung injury Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/832f9488ac954c8fa501011a2a7d8000
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spelling	oai:doaj.org-article:832f9488ac954c8fa501011a2a7d80002021-11-16T04:09:29ZPulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines1878-535210.1016/j.arabjc.2021.103533https://doaj.org/article/832f9488ac954c8fa501011a2a7d80002022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221005487https://doaj.org/toc/1878-5352Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the synthesis of Au nanoparticles by pectin (PEC) as a natural reducing and stabilizing agent without using any toxic and harmful reagent under ultrasonic condition. The biosynthesized Au NPs@PEC were characterized by advanced physicochemical techniques like ultraviolet–visible (UV–Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that pectin-stabilized Au nanoparticles have a spherical shape with a mean diameter from 5 to 10 nm. In the medicinal part of the present research, the lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell viability was determined by trypan blue assay. The caspase activity colorimetric assay kit and Rhodamine123 fluorescence dye were used to determine the caspase-3 activity and mitochondrial membrane potential, respectively. Apoptosis and DNA fragmentation were determined by the TUNEL test. Also, the inflammatory cytokines concentrations were evaluated by the Rat inflammatory cytokine assay kit. Au NPs@PEC-treated cell cutlers decreased significantly (p ≤ 0.01) the caspase-3 activity, inflammatory cytokines concentrations, and DNA fragmentation, and enhanced the mitochondrial membrane potential and cell viability in the high concentration of Methotrexate-treated lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cells. In the antioxidant test, the IC50 of Au NPs@PEC nanocomposite and BHT against DPPH free radicals were 203 and 181 µg/mL, respectively. Finally, Au NPs@PEC may be used as a pulmonary protective supplement to treat acute lung injury.Dongxiao WangJin PuYuebin LiaoJinhua LiuGen HuElsevierarticlePectinGold nanoparticlesDNA fragmentationApoptosisAntioxidantAcute lung injuryChemistryQD1-999ENArabian Journal of Chemistry, Vol 15, Iss 1, Pp 103533- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pectin Gold nanoparticles DNA fragmentation Apoptosis Antioxidant Acute lung injury Chemistry QD1-999
spellingShingle	Pectin Gold nanoparticles DNA fragmentation Apoptosis Antioxidant Acute lung injury Chemistry QD1-999 Dongxiao Wang Jin Pu Yuebin Liao Jinhua Liu Gen Hu Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
description	Regarding applicative, facile, green chemical research, a bio-inspired approach is being reported for the synthesis of Au nanoparticles by pectin (PEC) as a natural reducing and stabilizing agent without using any toxic and harmful reagent under ultrasonic condition. The biosynthesized Au NPs@PEC were characterized by advanced physicochemical techniques like ultraviolet–visible (UV–Vis), Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX), and X-ray Diffraction (XRD) study. It has been established that pectin-stabilized Au nanoparticles have a spherical shape with a mean diameter from 5 to 10 nm. In the medicinal part of the present research, the lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell viability was determined by trypan blue assay. The caspase activity colorimetric assay kit and Rhodamine123 fluorescence dye were used to determine the caspase-3 activity and mitochondrial membrane potential, respectively. Apoptosis and DNA fragmentation were determined by the TUNEL test. Also, the inflammatory cytokines concentrations were evaluated by the Rat inflammatory cytokine assay kit. Au NPs@PEC-treated cell cutlers decreased significantly (p ≤ 0.01) the caspase-3 activity, inflammatory cytokines concentrations, and DNA fragmentation, and enhanced the mitochondrial membrane potential and cell viability in the high concentration of Methotrexate-treated lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cells. In the antioxidant test, the IC50 of Au NPs@PEC nanocomposite and BHT against DPPH free radicals were 203 and 181 µg/mL, respectively. Finally, Au NPs@PEC may be used as a pulmonary protective supplement to treat acute lung injury.
format	article
author	Dongxiao Wang Jin Pu Yuebin Liao Jinhua Liu Gen Hu
author_facet	Dongxiao Wang Jin Pu Yuebin Liao Jinhua Liu Gen Hu
author_sort	Dongxiao Wang
title	Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
title_short	Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
title_full	Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
title_fullStr	Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
title_full_unstemmed	Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines
title_sort	pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on methotrexate-induced acute lung injury in lung beas-2b, wi-38, ccd-19lu, imr-90, mrc-5, and hel 299 cell lines
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/832f9488ac954c8fa501011a2a7d8000
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Pulmonary protective effects of ultrasonic green synthesis of gold nanoparticles mediated by pectin on Methotrexate-induced acute lung injury in lung BEAS-2B, WI-38, CCD-19Lu, IMR-90, MRC-5, and HEL 299 cell lines

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