Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.

Previously, we have identified a novel CCCH zinc finger protein family as negative regulators of macrophage activation. To gain an overall insight into the entire CCCH zinc finger gene family and to evaluate their potential role in macrophage activation, here we performed a genome-wide survey of CCC...

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Autores principales:	Jian Liang, Wenjun Song, Gail Tromp, Pappachan E Kolattukudy, Mingui Fu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/833773d9dd944cb7996d3c52334874f6
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spelling	oai:doaj.org-article:833773d9dd944cb7996d3c52334874f62021-11-25T06:11:21ZGenome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.1932-620310.1371/journal.pone.0002880https://doaj.org/article/833773d9dd944cb7996d3c52334874f62008-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18682727/?tool=EBIhttps://doaj.org/toc/1932-6203Previously, we have identified a novel CCCH zinc finger protein family as negative regulators of macrophage activation. To gain an overall insight into the entire CCCH zinc finger gene family and to evaluate their potential role in macrophage activation, here we performed a genome-wide survey of CCCH zinc finger genes in mouse and human. Totally 58 CCCH zinc finger genes in mouse and 55 in human were identified and most of them have not been reported previously. Phylogenetic analysis revealed that the mouse CCCH family was divided into 6 groups. Meanwhile, we employed quantitative real-time PCR to profile their tissue expression patterns in adult mice. Clustering analysis showed that most of CCCH genes were broadly expressed in all of tissues examined with various levels. Interestingly, several CCCH genes Mbnl3, Zfp36l2, Zfp36, Zc3h12a, Zc3h12d, Zc3h7a and Leng9 were enriched in macrophage-related organs such as thymus, spleen, lung, intestine and adipose. Consistently, a comprehensive assessment of changes in expression of the 58 members of the mouse CCCH family during macrophage activation also revealed that these CCCH zinc finger genes were associated with the activation of bone marrow-derived macrophages by lipopolysaccharide. Taken together, this study not only identified a functional module of CCCH zinc finger genes in the regulation of macrophage activation but also provided the framework for future studies to dissect the function of this emerging gene family.Jian LiangWenjun SongGail TrompPappachan E KolattukudyMingui FuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 8, p e2880 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jian Liang Wenjun Song Gail Tromp Pappachan E Kolattukudy Mingui Fu Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
description	Previously, we have identified a novel CCCH zinc finger protein family as negative regulators of macrophage activation. To gain an overall insight into the entire CCCH zinc finger gene family and to evaluate their potential role in macrophage activation, here we performed a genome-wide survey of CCCH zinc finger genes in mouse and human. Totally 58 CCCH zinc finger genes in mouse and 55 in human were identified and most of them have not been reported previously. Phylogenetic analysis revealed that the mouse CCCH family was divided into 6 groups. Meanwhile, we employed quantitative real-time PCR to profile their tissue expression patterns in adult mice. Clustering analysis showed that most of CCCH genes were broadly expressed in all of tissues examined with various levels. Interestingly, several CCCH genes Mbnl3, Zfp36l2, Zfp36, Zc3h12a, Zc3h12d, Zc3h7a and Leng9 were enriched in macrophage-related organs such as thymus, spleen, lung, intestine and adipose. Consistently, a comprehensive assessment of changes in expression of the 58 members of the mouse CCCH family during macrophage activation also revealed that these CCCH zinc finger genes were associated with the activation of bone marrow-derived macrophages by lipopolysaccharide. Taken together, this study not only identified a functional module of CCCH zinc finger genes in the regulation of macrophage activation but also provided the framework for future studies to dissect the function of this emerging gene family.
format	article
author	Jian Liang Wenjun Song Gail Tromp Pappachan E Kolattukudy Mingui Fu
author_facet	Jian Liang Wenjun Song Gail Tromp Pappachan E Kolattukudy Mingui Fu
author_sort	Jian Liang
title	Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
title_short	Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
title_full	Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
title_fullStr	Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
title_full_unstemmed	Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.
title_sort	genome-wide survey and expression profiling of ccch-zinc finger family reveals a functional module in macrophage activation.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/833773d9dd944cb7996d3c52334874f6
work_keys_str_mv	AT jianliang genomewidesurveyandexpressionprofilingofccchzincfingerfamilyrevealsafunctionalmoduleinmacrophageactivation AT wenjunsong genomewidesurveyandexpressionprofilingofccchzincfingerfamilyrevealsafunctionalmoduleinmacrophageactivation AT gailtromp genomewidesurveyandexpressionprofilingofccchzincfingerfamilyrevealsafunctionalmoduleinmacrophageactivation AT pappachanekolattukudy genomewidesurveyandexpressionprofilingofccchzincfingerfamilyrevealsafunctionalmoduleinmacrophageactivation AT minguifu genomewidesurveyandexpressionprofilingofccchzincfingerfamilyrevealsafunctionalmoduleinmacrophageactivation
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Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation.

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