Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis

Hypoxia is common in marine environments and a major stressor for marine organisms inhabiting benthic and intertidal zones. Several studies have explored the responses of these organisms to hypoxic stress at the whole organism level with a focus on energy metabolism and mitochondrial response, but t...

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Autores principales:	Eugene P. Sokolov, Linda Adzigbli, Stephanie Markert, Amanda Bundgaard, Angela Fago, Dörte Becher, Claudia Hirschfeld, Inna M. Sokolova
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	bioenergetics mitochondria respiration oxidative stress proteomics posttranslational modification (PTM) Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/833ae66eacfc4998bd005a97becdceac
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spelling	oai:doaj.org-article:833ae66eacfc4998bd005a97becdceac2021-11-05T05:46:51ZIntrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis2296-774510.3389/fmars.2021.773734https://doaj.org/article/833ae66eacfc4998bd005a97becdceac2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.773734/fullhttps://doaj.org/toc/2296-7745Hypoxia is common in marine environments and a major stressor for marine organisms inhabiting benthic and intertidal zones. Several studies have explored the responses of these organisms to hypoxic stress at the whole organism level with a focus on energy metabolism and mitochondrial response, but the instrinsic mitochondrial responses that support the organelle’s function under hypoxia and reoxygenation (H/R) stress are not well understood. We studied the effects of acute H/R stress (10 min anoxia followed by 15 min reoxygenation) on mitochondrial respiration, production of reactive oxygen species (ROS) and posttranslational modifications (PTM) of the proteome in a marine facultative anaerobe, the blue mussel Mytilus edulis. The mussels’ mitochondria showed increased OXPHOS respiration and suppressed proton leak resulting in a higher coupling efficiency after H/R stress. ROS production decreased in both the resting (LEAK) and phosphorylating (OXPHOS) state indicating that M. edulis was able to prevent oxidative stress and mitochondrial damage during reoxygenation. Hypoxia did not lead to rearrangement of the mitochondrial supercomplexes but impacted the mitochondrial phosphoproteome including the proteins involved in OXPHOS, amino acid- and fatty acid catabolism, and protein quality control. This study indicates that mussels’ mitochondria possess intrinsic mechanisms (including regulation via reversible protein phosphorylation) that ensure high respiratory flux and mitigate oxidative damage during H/R stress and contribute to the hypoxia-tolerant mitochondrial phenotype of this metabolically plastic species.Eugene P. SokolovLinda AdzigbliLinda AdzigbliStephanie MarkertStephanie MarkertAmanda BundgaardAngela FagoDörte BecherClaudia HirschfeldInna M. SokolovaInna M. SokolovaFrontiers Media S.A.articlebioenergeticsmitochondriarespirationoxidative stressproteomicsposttranslational modification (PTM)ScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioenergetics mitochondria respiration oxidative stress proteomics posttranslational modification (PTM) Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	bioenergetics mitochondria respiration oxidative stress proteomics posttranslational modification (PTM) Science Q General. Including nature conservation, geographical distribution QH1-199.5 Eugene P. Sokolov Linda Adzigbli Linda Adzigbli Stephanie Markert Stephanie Markert Amanda Bundgaard Angela Fago Dörte Becher Claudia Hirschfeld Inna M. Sokolova Inna M. Sokolova Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis
description	Hypoxia is common in marine environments and a major stressor for marine organisms inhabiting benthic and intertidal zones. Several studies have explored the responses of these organisms to hypoxic stress at the whole organism level with a focus on energy metabolism and mitochondrial response, but the instrinsic mitochondrial responses that support the organelle’s function under hypoxia and reoxygenation (H/R) stress are not well understood. We studied the effects of acute H/R stress (10 min anoxia followed by 15 min reoxygenation) on mitochondrial respiration, production of reactive oxygen species (ROS) and posttranslational modifications (PTM) of the proteome in a marine facultative anaerobe, the blue mussel Mytilus edulis. The mussels’ mitochondria showed increased OXPHOS respiration and suppressed proton leak resulting in a higher coupling efficiency after H/R stress. ROS production decreased in both the resting (LEAK) and phosphorylating (OXPHOS) state indicating that M. edulis was able to prevent oxidative stress and mitochondrial damage during reoxygenation. Hypoxia did not lead to rearrangement of the mitochondrial supercomplexes but impacted the mitochondrial phosphoproteome including the proteins involved in OXPHOS, amino acid- and fatty acid catabolism, and protein quality control. This study indicates that mussels’ mitochondria possess intrinsic mechanisms (including regulation via reversible protein phosphorylation) that ensure high respiratory flux and mitigate oxidative damage during H/R stress and contribute to the hypoxia-tolerant mitochondrial phenotype of this metabolically plastic species.
format	article
author	Eugene P. Sokolov Linda Adzigbli Linda Adzigbli Stephanie Markert Stephanie Markert Amanda Bundgaard Angela Fago Dörte Becher Claudia Hirschfeld Inna M. Sokolova Inna M. Sokolova
author_facet	Eugene P. Sokolov Linda Adzigbli Linda Adzigbli Stephanie Markert Stephanie Markert Amanda Bundgaard Angela Fago Dörte Becher Claudia Hirschfeld Inna M. Sokolova Inna M. Sokolova
author_sort	Eugene P. Sokolov
title	Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis
title_short	Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis
title_full	Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis
title_fullStr	Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis
title_full_unstemmed	Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis
title_sort	intrinsic mechanisms underlying hypoxia-tolerant mitochondrial phenotype during hypoxia-reoxygenation stress in a marine facultative anaerobe, the blue mussel mytilus edulis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/833ae66eacfc4998bd005a97becdceac
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Intrinsic Mechanisms Underlying Hypoxia-Tolerant Mitochondrial Phenotype During Hypoxia-Reoxygenation Stress in a Marine Facultative Anaerobe, the Blue Mussel Mytilus edulis

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