Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer

Methyltransferase like 3 (METTL3) has been identified to serve as a definitive inducer in cancer progression. This study sought to analyze the regulatory mechanism of METTL3 in epithelial-mesenchymal transition (EMT), invasion, and metastasis in esophageal cancer (ESCA). The METTL3 expressions in ca...

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Autores principales:	Xuyang Liang, Zhimei Zhang, Lu Wang, Shuxian Zhang, Ling Ren, Shouying Li, Jing Xu, Shengxiang Lv
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	esophageal cancer mettl3 m6a modification dgcr8 pri-mir-20a-5p mir-20a-5p nfic epithelial-mesenchymal transition Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/83461020d9fb4d3f82151f336aa10945
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spelling	oai:doaj.org-article:83461020d9fb4d3f82151f336aa109452021-12-01T14:41:00ZMechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer2165-59792165-598710.1080/21655979.2021.1994721https://doaj.org/article/83461020d9fb4d3f82151f336aa109452021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1994721https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Methyltransferase like 3 (METTL3) has been identified to serve as a definitive inducer in cancer progression. This study sought to analyze the regulatory mechanism of METTL3 in epithelial-mesenchymal transition (EMT), invasion, and metastasis in esophageal cancer (ESCA). The METTL3 expressions in cancer tissues and cells were detected with extensive analysis of its correlation with clinical baseline data. The cells were transfected with sh-RNA-METTL3 and microRNA (miR)-20a-5p mimic, followed by evaluation of invasion, migration, and EMT. The N6-methyladenosine (m6A) level and enrichment of DiGeorge Critical Region 8 (DGCR8) and m6A were observed. The binding relationship between miR-20a-5p and Nuclear Factor I-C (NFIC) was verified, followed by Pearson correlation analysis. A subcutaneous tumor formation assay was conducted prior to observation of lung metastases. Our results revealed that METTL3 was highly expressed in ESCA patients and associated with severe lymph node involvement and distant metastasis. METTL3 downregulation radically inhibited the invasiveness, migration, and EMT. METTL3 elevated the miR-20a-5p expression via improving m6A modification. METTL3 inhibition downregulated the miR-20a-5p expression. Moreover, miR-20a-5p upregulation facilitated ESCA cell invasiveness and migration by targeting NFIC transcription. METTL3 inhibition suppressed tumor growth and lung metastasis in vivo. Overall, METTL3 promoted m6A modification and the binding of DGCR8 to miR-20a-5p to further elevate the miR-20a-5p expression and inhibit NFIC transcription, thus promoting EMT, invasion and migration.Xuyang LiangZhimei ZhangLu WangShuxian ZhangLing RenShouying LiJing XuShengxiang LvTaylor & Francis Grouparticleesophageal cancermettl3m6a modificationdgcr8pri-mir-20a-5pmir-20a-5pnficepithelial-mesenchymal transitionBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 10023-10036 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	esophageal cancer mettl3 m6a modification dgcr8 pri-mir-20a-5p mir-20a-5p nfic epithelial-mesenchymal transition Biotechnology TP248.13-248.65
spellingShingle	esophageal cancer mettl3 m6a modification dgcr8 pri-mir-20a-5p mir-20a-5p nfic epithelial-mesenchymal transition Biotechnology TP248.13-248.65 Xuyang Liang Zhimei Zhang Lu Wang Shuxian Zhang Ling Ren Shouying Li Jing Xu Shengxiang Lv Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
description	Methyltransferase like 3 (METTL3) has been identified to serve as a definitive inducer in cancer progression. This study sought to analyze the regulatory mechanism of METTL3 in epithelial-mesenchymal transition (EMT), invasion, and metastasis in esophageal cancer (ESCA). The METTL3 expressions in cancer tissues and cells were detected with extensive analysis of its correlation with clinical baseline data. The cells were transfected with sh-RNA-METTL3 and microRNA (miR)-20a-5p mimic, followed by evaluation of invasion, migration, and EMT. The N6-methyladenosine (m6A) level and enrichment of DiGeorge Critical Region 8 (DGCR8) and m6A were observed. The binding relationship between miR-20a-5p and Nuclear Factor I-C (NFIC) was verified, followed by Pearson correlation analysis. A subcutaneous tumor formation assay was conducted prior to observation of lung metastases. Our results revealed that METTL3 was highly expressed in ESCA patients and associated with severe lymph node involvement and distant metastasis. METTL3 downregulation radically inhibited the invasiveness, migration, and EMT. METTL3 elevated the miR-20a-5p expression via improving m6A modification. METTL3 inhibition downregulated the miR-20a-5p expression. Moreover, miR-20a-5p upregulation facilitated ESCA cell invasiveness and migration by targeting NFIC transcription. METTL3 inhibition suppressed tumor growth and lung metastasis in vivo. Overall, METTL3 promoted m6A modification and the binding of DGCR8 to miR-20a-5p to further elevate the miR-20a-5p expression and inhibit NFIC transcription, thus promoting EMT, invasion and migration.
format	article
author	Xuyang Liang Zhimei Zhang Lu Wang Shuxian Zhang Ling Ren Shouying Li Jing Xu Shengxiang Lv
author_facet	Xuyang Liang Zhimei Zhang Lu Wang Shuxian Zhang Ling Ren Shouying Li Jing Xu Shengxiang Lv
author_sort	Xuyang Liang
title	Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
title_short	Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
title_full	Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
title_fullStr	Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
title_full_unstemmed	Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
title_sort	mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/83461020d9fb4d3f82151f336aa10945
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_version_	1718405003763253248

Mechanism of methyltransferase like 3 in epithelial-mesenchymal transition process, invasion, and metastasis in esophageal cancer

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