Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens

Photodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with r...

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Autores principales:	Nadezhda Ignatova, Tatiana Ivanova, Artem Antonyan, Ivan Budruev, Olga Streltsova, Vadim Elagin, Vladislav Kamensky
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	urolithiasis antibiotic-resistant bacteria bactericidal effect laser-induced inactivation photosensitizer Applied optics. Photonics TA1501-1820
Acceso en línea:	https://doaj.org/article/83474888f87e42b9a5a7b3af4b6a767e
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spelling	oai:doaj.org-article:83474888f87e42b9a5a7b3af4b6a767e2021-11-25T18:43:22ZEfficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens10.3390/photonics81104952304-6732https://doaj.org/article/83474888f87e42b9a5a7b3af4b6a767e2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-6732/8/11/495https://doaj.org/toc/2304-6732Photodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with renal calculi were isolated and identified using standard methods of bacteriological analysis and tested for drug resistance to 10 antibiotics by the disco-diffusion method. Uropathogenic bacterial strains present in 78.7 ± 5.2% of the infected samples from the total number of analyzed calculi. The most frequent representatives belonged to the genera <i>Staphylococcus</i>, <i>Escherichia</i>, and <i>Enterococcus</i>. All tested strains showed high antibiotic resistance. Representatives of the most common bacterial genera in the calculi were used as models for the selection of PD exposure modes. It was found that the maximum time of photosensitizer accumulation depends on the structure of the bacterial cell wall: 30 min for Gram-negative strains and 60 min for Gram-positive ones. Optimal modes of PD exposure to antibiotic-resistant uropathogenic microorganisms were selected: 50 µg/mL Fotoditazin and 150 mW laser power. The maximal bactericidal activity of PDI against uropathogenic microorganisms was shown for <i>Enterococcus faecalis</i>, and <i>Staphylococcus aureus</i>. The bacteriostatic effect was found against <i>Escherichia coli</i> and <i>Proteus mirabilis.</i>Nadezhda IgnatovaTatiana IvanovaArtem AntonyanIvan BudruevOlga StreltsovaVadim ElaginVladislav KamenskyMDPI AGarticleurolithiasisantibiotic-resistant bacteriabactericidal effectlaser-induced inactivationphotosensitizerApplied optics. PhotonicsTA1501-1820ENPhotonics, Vol 8, Iss 495, p 495 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urolithiasis antibiotic-resistant bacteria bactericidal effect laser-induced inactivation photosensitizer Applied optics. Photonics TA1501-1820
spellingShingle	urolithiasis antibiotic-resistant bacteria bactericidal effect laser-induced inactivation photosensitizer Applied optics. Photonics TA1501-1820 Nadezhda Ignatova Tatiana Ivanova Artem Antonyan Ivan Budruev Olga Streltsova Vadim Elagin Vladislav Kamensky Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
description	Photodynamic inactivation (PDI) is considered to be an effective method of prevention of postoperative complications of urolithiasis. The present study shows a complex approach to assess the efficacy of PDI of drug resistant bacteria associated with renal calculi. Bacterial strains associated with renal calculi were isolated and identified using standard methods of bacteriological analysis and tested for drug resistance to 10 antibiotics by the disco-diffusion method. Uropathogenic bacterial strains present in 78.7 ± 5.2% of the infected samples from the total number of analyzed calculi. The most frequent representatives belonged to the genera <i>Staphylococcus</i>, <i>Escherichia</i>, and <i>Enterococcus</i>. All tested strains showed high antibiotic resistance. Representatives of the most common bacterial genera in the calculi were used as models for the selection of PD exposure modes. It was found that the maximum time of photosensitizer accumulation depends on the structure of the bacterial cell wall: 30 min for Gram-negative strains and 60 min for Gram-positive ones. Optimal modes of PD exposure to antibiotic-resistant uropathogenic microorganisms were selected: 50 µg/mL Fotoditazin and 150 mW laser power. The maximal bactericidal activity of PDI against uropathogenic microorganisms was shown for <i>Enterococcus faecalis</i>, and <i>Staphylococcus aureus</i>. The bacteriostatic effect was found against <i>Escherichia coli</i> and <i>Proteus mirabilis.</i>
format	article
author	Nadezhda Ignatova Tatiana Ivanova Artem Antonyan Ivan Budruev Olga Streltsova Vadim Elagin Vladislav Kamensky
author_facet	Nadezhda Ignatova Tatiana Ivanova Artem Antonyan Ivan Budruev Olga Streltsova Vadim Elagin Vladislav Kamensky
author_sort	Nadezhda Ignatova
title	Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
title_short	Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
title_full	Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
title_fullStr	Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
title_full_unstemmed	Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens
title_sort	efficacy of photodynamic inactivation against the major human antibiotic-resistant uropathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/83474888f87e42b9a5a7b3af4b6a767e
work_keys_str_mv	AT nadezhdaignatova efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens AT tatianaivanova efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens AT artemantonyan efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens AT ivanbudruev efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens AT olgastreltsova efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens AT vadimelagin efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens AT vladislavkamensky efficacyofphotodynamicinactivationagainstthemajorhumanantibioticresistanturopathogens
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Efficacy of Photodynamic Inactivation against the Major Human Antibiotic-Resistant Uropathogens

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