Epigenetic signature of chronic low back pain in human T cells

Abstract. Objective:. Determine if chronic low back pain (LBP) is associated with DNA methylation signatures in human T cells that will reveal novel mechanisms and potential therapeutic targets and explore the feasibility of epigenetic diagnostic markers for pain-related pathophysiology. Methods:. G...

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Autores principales:	Stéphanie Grégoire, David Cheishvili, Mali Salmon-Divon, Sergiy Dymov, Lucas Topham, Virginie Calderon, Yoram Shir, Moshe Szyf, Laura S. Stone
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer 2021
Materias:	Anesthesiology RD78.3-87.3
Acceso en línea:	https://doaj.org/article/834bfd8300a94aec92e6c133920966ed
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spelling	oai:doaj.org-article:834bfd8300a94aec92e6c133920966ed2021-11-25T07:59:39ZEpigenetic signature of chronic low back pain in human T cells2471-253110.1097/PR9.0000000000000960https://doaj.org/article/834bfd8300a94aec92e6c133920966ed2021-11-01T00:00:00Zhttp://journals.lww.com/painrpts/fulltext/10.1097/PR9.0000000000000960https://doaj.org/toc/2471-2531Abstract. Objective:. Determine if chronic low back pain (LBP) is associated with DNA methylation signatures in human T cells that will reveal novel mechanisms and potential therapeutic targets and explore the feasibility of epigenetic diagnostic markers for pain-related pathophysiology. Methods:. Genome-wide DNA methylation analysis of 850,000 CpG sites in women and men with chronic LBP and pain-free controls was performed. T cells were isolated (discovery cohort, n = 32) and used to identify differentially methylated CpG sites, and gene ontologies and molecular pathways were identified. A polygenic DNA methylation score for LBP was generated in both women and men. Validation was performed in an independent cohort (validation cohort, n = 63) of chronic LBP and healthy controls. Results:. Analysis with the discovery cohort revealed a total of 2,496 and 419 differentially methylated CpGs in women and men, respectively. In women, most of these sites were hypomethylated and enriched in genes with functions in the extracellular matrix, in the immune system (ie, cytokines), or in epigenetic processes. In men, a unique chronic LBP DNA methylation signature was identified characterized by significant enrichment for genes from the major histocompatibility complex. Sex-specific polygenic DNA methylation scores were generated to estimate the pain status of each individual and confirmed in the validation cohort using pyrosequencing. Conclusion:. This study reveals sex-specific DNA methylation signatures in human T cells that discriminates chronic LBP participants from healthy controls.Stéphanie GrégoireDavid CheishviliMali Salmon-DivonSergiy DymovLucas TophamVirginie CalderonYoram ShirMoshe SzyfLaura S. StoneWolters KluwerarticleAnesthesiologyRD78.3-87.3ENPAIN Reports, Vol 6, Iss 4, p e960 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anesthesiology RD78.3-87.3
spellingShingle	Anesthesiology RD78.3-87.3 Stéphanie Grégoire David Cheishvili Mali Salmon-Divon Sergiy Dymov Lucas Topham Virginie Calderon Yoram Shir Moshe Szyf Laura S. Stone Epigenetic signature of chronic low back pain in human T cells
description	Abstract. Objective:. Determine if chronic low back pain (LBP) is associated with DNA methylation signatures in human T cells that will reveal novel mechanisms and potential therapeutic targets and explore the feasibility of epigenetic diagnostic markers for pain-related pathophysiology. Methods:. Genome-wide DNA methylation analysis of 850,000 CpG sites in women and men with chronic LBP and pain-free controls was performed. T cells were isolated (discovery cohort, n = 32) and used to identify differentially methylated CpG sites, and gene ontologies and molecular pathways were identified. A polygenic DNA methylation score for LBP was generated in both women and men. Validation was performed in an independent cohort (validation cohort, n = 63) of chronic LBP and healthy controls. Results:. Analysis with the discovery cohort revealed a total of 2,496 and 419 differentially methylated CpGs in women and men, respectively. In women, most of these sites were hypomethylated and enriched in genes with functions in the extracellular matrix, in the immune system (ie, cytokines), or in epigenetic processes. In men, a unique chronic LBP DNA methylation signature was identified characterized by significant enrichment for genes from the major histocompatibility complex. Sex-specific polygenic DNA methylation scores were generated to estimate the pain status of each individual and confirmed in the validation cohort using pyrosequencing. Conclusion:. This study reveals sex-specific DNA methylation signatures in human T cells that discriminates chronic LBP participants from healthy controls.
format	article
author	Stéphanie Grégoire David Cheishvili Mali Salmon-Divon Sergiy Dymov Lucas Topham Virginie Calderon Yoram Shir Moshe Szyf Laura S. Stone
author_facet	Stéphanie Grégoire David Cheishvili Mali Salmon-Divon Sergiy Dymov Lucas Topham Virginie Calderon Yoram Shir Moshe Szyf Laura S. Stone
author_sort	Stéphanie Grégoire
title	Epigenetic signature of chronic low back pain in human T cells
title_short	Epigenetic signature of chronic low back pain in human T cells
title_full	Epigenetic signature of chronic low back pain in human T cells
title_fullStr	Epigenetic signature of chronic low back pain in human T cells
title_full_unstemmed	Epigenetic signature of chronic low back pain in human T cells
title_sort	epigenetic signature of chronic low back pain in human t cells
publisher	Wolters Kluwer
publishDate	2021
url	https://doaj.org/article/834bfd8300a94aec92e6c133920966ed
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Epigenetic signature of chronic low back pain in human T cells

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