TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells

Abstract Combination antiretroviral therapy (cART) has increased the life expectancy of HIV patients. However, the incidence of non-AIDS associated lung comorbidities, such as COPD and asthma, and that of opportunistic lung infections have become more common among this population. HIV proteins secre...

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Autores principales:	S. Chinnapaiyan, R. K. Dutta, M. Nair, H. S. Chand, I. Rahman, H. J. Unwalla
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/834ec782dc1b4dcebf44bd65ff31e1a1
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spelling	oai:doaj.org-article:834ec782dc1b4dcebf44bd65ff31e1a12021-12-02T15:08:20ZTGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells10.1038/s41598-019-49056-62045-2322https://doaj.org/article/834ec782dc1b4dcebf44bd65ff31e1a12019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-49056-6https://doaj.org/toc/2045-2322Abstract Combination antiretroviral therapy (cART) has increased the life expectancy of HIV patients. However, the incidence of non-AIDS associated lung comorbidities, such as COPD and asthma, and that of opportunistic lung infections have become more common among this population. HIV proteins secreted by the anatomical HIV reservoirs can have both autocrine and paracrine effects contributing to the HIV-associated comorbidities. HIV has been recovered from cell-free bronchoalveolar lavage fluid, alveolar macrophages, and intrapulmonary lymphocytes. We have recently shown that ex-vivo cultured primary bronchial epithelial cells and the bronchial brushings from human subjects express canonical HIV receptors CD4, CCR5 and CXCR4 and can be infected with HIV. Together these studies suggest that the lung tissue can serve as an important reservoir for HIV. In this report, we show that TGF-β1 promotes HIV latency by upregulating a transcriptional repressor BLIMP-1. Furthermore, we identify miR-9-5p as an important intermediate in TGF-β-mediated BLIMP-1 upregulation and consequent HIV latency. The transcriptionally suppressed HIV can be reactivated by common latency reactivating agents. Together our data suggest that in patients with chronic airway diseases, TGF-β can elevate the HIV viral reservoir load that could further exacerbate the HIV associated lung comorbidities.S. ChinnapaiyanR. K. DuttaM. NairH. S. ChandI. RahmanH. J. UnwallaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Chinnapaiyan R. K. Dutta M. Nair H. S. Chand I. Rahman H. J. Unwalla TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells
description	Abstract Combination antiretroviral therapy (cART) has increased the life expectancy of HIV patients. However, the incidence of non-AIDS associated lung comorbidities, such as COPD and asthma, and that of opportunistic lung infections have become more common among this population. HIV proteins secreted by the anatomical HIV reservoirs can have both autocrine and paracrine effects contributing to the HIV-associated comorbidities. HIV has been recovered from cell-free bronchoalveolar lavage fluid, alveolar macrophages, and intrapulmonary lymphocytes. We have recently shown that ex-vivo cultured primary bronchial epithelial cells and the bronchial brushings from human subjects express canonical HIV receptors CD4, CCR5 and CXCR4 and can be infected with HIV. Together these studies suggest that the lung tissue can serve as an important reservoir for HIV. In this report, we show that TGF-β1 promotes HIV latency by upregulating a transcriptional repressor BLIMP-1. Furthermore, we identify miR-9-5p as an important intermediate in TGF-β-mediated BLIMP-1 upregulation and consequent HIV latency. The transcriptionally suppressed HIV can be reactivated by common latency reactivating agents. Together our data suggest that in patients with chronic airway diseases, TGF-β can elevate the HIV viral reservoir load that could further exacerbate the HIV associated lung comorbidities.
format	article
author	S. Chinnapaiyan R. K. Dutta M. Nair H. S. Chand I. Rahman H. J. Unwalla
author_facet	S. Chinnapaiyan R. K. Dutta M. Nair H. S. Chand I. Rahman H. J. Unwalla
author_sort	S. Chinnapaiyan
title	TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells
title_short	TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells
title_full	TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells
title_fullStr	TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells
title_full_unstemmed	TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells
title_sort	tgf-β1 increases viral burden and promotes hiv-1 latency in primary differentiated human bronchial epithelial cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/834ec782dc1b4dcebf44bd65ff31e1a1
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TGF-β1 increases viral burden and promotes HIV-1 latency in primary differentiated human bronchial epithelial cells

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