Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the highly infectious coronavirus disease COVID-19. Extensive research has been performed in recent months to better understand how SARS-CoV-2 infects and ma-nipulates its host to identify potential drug targets a...

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Autores principales:	Cinzia Klemm, Henry Wood, Grace Heredge Thomas, Guðjón Ólafsson, Mara Teixeira Torres, Peter H. Thorpe
Formato:	article
Lenguaje:	EN
Publicado:	Shared Science Publishers OG 2021
Materias:	sars-cov-2 covid-19 synthetic physical interactions vesicle trafficking transcription Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/83532c3e718c44c5865eb0d261090653
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spelling	oai:doaj.org-article:83532c3e718c44c5865eb0d2610906532021-12-03T12:59:37ZForced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking2311-263810.15698/mic2021.12.766https://doaj.org/article/83532c3e718c44c5865eb0d2610906532021-10-01T00:00:00Zhttp://microbialcell.com/researcharticles/2021a-klemm-microbial-cell/https://doaj.org/toc/2311-2638Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the highly infectious coronavirus disease COVID-19. Extensive research has been performed in recent months to better understand how SARS-CoV-2 infects and ma-nipulates its host to identify potential drug targets and support patient recovery from COVID-19. However, the function of many SARS-CoV-2 proteins remains uncharacterised. Here we used the Synthetic Physical Interactions (SPI) method to recruit SARS-CoV-2 proteins to most of the budding yeast proteome to identify conserved pathways which are affected by SARS-CoV-2 proteins. The set of yeast proteins that result in growth defects when asso-ciated with the viral proteins have homologous functions that overlap those identified in studies performed in mammalian cells. Specifically, we were able to show that recruiting the SARS-CoV-2 NSP1 protein to HOPS, a vesicle-docking complex, is sufficient to perturb membrane trafficking in yeast consistent with the hijack-ing of the endoplasmic-reticulum–Golgi intermediate compart-ment trafficking pathway during viral infection of mammalian cells. These data demonstrate that the yeast SPI method is a rap-id way to identify potential functions of ectopic viral proteins.Cinzia KlemmHenry WoodGrace Heredge ThomasGuðjón ÓlafssonMara Teixeira TorresPeter H. ThorpeShared Science Publishers OGarticlesars-cov-2covid-19synthetic physical interactionsvesicle traffickingtranscriptionBiology (General)QH301-705.5ENMicrobial Cell, Vol 8, Iss 12, Pp 280-296 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sars-cov-2 covid-19 synthetic physical interactions vesicle trafficking transcription Biology (General) QH301-705.5
spellingShingle	sars-cov-2 covid-19 synthetic physical interactions vesicle trafficking transcription Biology (General) QH301-705.5 Cinzia Klemm Henry Wood Grace Heredge Thomas Guðjón Ólafsson Mara Teixeira Torres Peter H. Thorpe Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
description	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the highly infectious coronavirus disease COVID-19. Extensive research has been performed in recent months to better understand how SARS-CoV-2 infects and ma-nipulates its host to identify potential drug targets and support patient recovery from COVID-19. However, the function of many SARS-CoV-2 proteins remains uncharacterised. Here we used the Synthetic Physical Interactions (SPI) method to recruit SARS-CoV-2 proteins to most of the budding yeast proteome to identify conserved pathways which are affected by SARS-CoV-2 proteins. The set of yeast proteins that result in growth defects when asso-ciated with the viral proteins have homologous functions that overlap those identified in studies performed in mammalian cells. Specifically, we were able to show that recruiting the SARS-CoV-2 NSP1 protein to HOPS, a vesicle-docking complex, is sufficient to perturb membrane trafficking in yeast consistent with the hijack-ing of the endoplasmic-reticulum–Golgi intermediate compart-ment trafficking pathway during viral infection of mammalian cells. These data demonstrate that the yeast SPI method is a rap-id way to identify potential functions of ectopic viral proteins.
format	article
author	Cinzia Klemm Henry Wood Grace Heredge Thomas Guðjón Ólafsson Mara Teixeira Torres Peter H. Thorpe
author_facet	Cinzia Klemm Henry Wood Grace Heredge Thomas Guðjón Ólafsson Mara Teixeira Torres Peter H. Thorpe
author_sort	Cinzia Klemm
title	Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
title_short	Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
title_full	Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
title_fullStr	Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
title_full_unstemmed	Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking
title_sort	forced association of sars-cov-2 proteins with the yeast proteome perturb vesicle trafficking
publisher	Shared Science Publishers OG
publishDate	2021
url	https://doaj.org/article/83532c3e718c44c5865eb0d261090653
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Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking

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