Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling

Abstract The high growth rate of Ulva seaweeds makes it a potential algal biomass resource. In particular, Ulva meridionalis grows up to fourfold a day. Here, we demonstrated strong carbon fixation by U. meridionalis using 13C stable isotope labelling and traced the 13C flux through sugar metabolite...

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Autores principales:	Shuntaro Tsubaki, Hiroshi Nishimura, Tomoya Imai, Ayumu Onda, Masanori Hiraoka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/835c0e27549a40f89e5ec798d4db3240
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spelling	oai:doaj.org-article:835c0e27549a40f89e5ec798d4db32402021-12-02T16:08:37ZProbing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling10.1038/s41598-020-77237-12045-2322https://doaj.org/article/835c0e27549a40f89e5ec798d4db32402020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77237-1https://doaj.org/toc/2045-2322Abstract The high growth rate of Ulva seaweeds makes it a potential algal biomass resource. In particular, Ulva meridionalis grows up to fourfold a day. Here, we demonstrated strong carbon fixation by U. meridionalis using 13C stable isotope labelling and traced the 13C flux through sugar metabolites with isotope-ratio mass spectrometry (IR-MS), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), 13C-nuclear magnetic resonance spectrometry (13C-NMR), and gas chromatography-mass spectrometry (GC–MS). U. meridionalis was first cultured in 13C-labelled enriched artificial seawater for 0–12 h, and the algae were collected every 4 h. U. meridionalis grew 1.8-fold (dry weight), and the 13C ratio reached 40% in 12 h, whereas 13C incorporation hardly occurred under darkness. At the beginning of the light period, 13C was incorporated into nucleic diphosphate (NDP) sugars in 4 h, and 13C labelled peaks were identified using FT-ICR-MS spectra. Using semiquantitative 13C-NMR measurements and GC–MS, 13C was detected in starch and matrix polysaccharides after the formation of NDP sugars. Moreover, the 14:10 light:dark regime resulted into 85% of 13C labelling was achieved after 72 h of cultivation. The rapid 13C uptake by U. meridionalis shows its strong carbon fixation capacity as a promising seaweed biomass feedstock.Shuntaro TsubakiHiroshi NishimuraTomoya ImaiAyumu OndaMasanori HiraokaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuntaro Tsubaki Hiroshi Nishimura Tomoya Imai Ayumu Onda Masanori Hiraoka Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling
description	Abstract The high growth rate of Ulva seaweeds makes it a potential algal biomass resource. In particular, Ulva meridionalis grows up to fourfold a day. Here, we demonstrated strong carbon fixation by U. meridionalis using 13C stable isotope labelling and traced the 13C flux through sugar metabolites with isotope-ratio mass spectrometry (IR-MS), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), 13C-nuclear magnetic resonance spectrometry (13C-NMR), and gas chromatography-mass spectrometry (GC–MS). U. meridionalis was first cultured in 13C-labelled enriched artificial seawater for 0–12 h, and the algae were collected every 4 h. U. meridionalis grew 1.8-fold (dry weight), and the 13C ratio reached 40% in 12 h, whereas 13C incorporation hardly occurred under darkness. At the beginning of the light period, 13C was incorporated into nucleic diphosphate (NDP) sugars in 4 h, and 13C labelled peaks were identified using FT-ICR-MS spectra. Using semiquantitative 13C-NMR measurements and GC–MS, 13C was detected in starch and matrix polysaccharides after the formation of NDP sugars. Moreover, the 14:10 light:dark regime resulted into 85% of 13C labelling was achieved after 72 h of cultivation. The rapid 13C uptake by U. meridionalis shows its strong carbon fixation capacity as a promising seaweed biomass feedstock.
format	article
author	Shuntaro Tsubaki Hiroshi Nishimura Tomoya Imai Ayumu Onda Masanori Hiraoka
author_facet	Shuntaro Tsubaki Hiroshi Nishimura Tomoya Imai Ayumu Onda Masanori Hiraoka
author_sort	Shuntaro Tsubaki
title	Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling
title_short	Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling
title_full	Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling
title_fullStr	Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling
title_full_unstemmed	Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling
title_sort	probing rapid carbon fixation in fast-growing seaweed ulva meridionalis using stable isotope 13c-labelling
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/835c0e27549a40f89e5ec798d4db3240
work_keys_str_mv	AT shuntarotsubaki probingrapidcarbonfixationinfastgrowingseaweedulvameridionalisusingstableisotope13clabelling AT hiroshinishimura probingrapidcarbonfixationinfastgrowingseaweedulvameridionalisusingstableisotope13clabelling AT tomoyaimai probingrapidcarbonfixationinfastgrowingseaweedulvameridionalisusingstableisotope13clabelling AT ayumuonda probingrapidcarbonfixationinfastgrowingseaweedulvameridionalisusingstableisotope13clabelling AT masanorihiraoka probingrapidcarbonfixationinfastgrowingseaweedulvameridionalisusingstableisotope13clabelling
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Probing rapid carbon fixation in fast-growing seaweed Ulva meridionalis using stable isotope 13C-labelling

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