Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing

Abstract Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing...

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Autores principales:	Norbert Moldován, Gábor Torma, Gábor Gulyás, Ákos Hornyák, Zoltán Zádori, Victoria A. Jefferson, Zsolt Csabai, Miklós Boldogkői, Dóra Tombácz, Florencia Meyer, Zsolt Boldogkői
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/836965dfd649493381b1c70a976eb07c
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spelling	oai:doaj.org-article:836965dfd649493381b1c70a976eb07c2021-12-02T15:09:23ZTime-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing10.1038/s41598-020-77520-12045-2322https://doaj.org/article/836965dfd649493381b1c70a976eb07c2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77520-1https://doaj.org/toc/2045-2322Abstract Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.Norbert MoldovánGábor TormaGábor GulyásÁkos HornyákZoltán ZádoriVictoria A. JeffersonZsolt CsabaiMiklós BoldogkőiDóra TombáczFlorencia MeyerZsolt BoldogkőiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Norbert Moldován Gábor Torma Gábor Gulyás Ákos Hornyák Zoltán Zádori Victoria A. Jefferson Zsolt Csabai Miklós Boldogkői Dóra Tombácz Florencia Meyer Zsolt Boldogkői Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
description	Abstract Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle worldwide. This study reports the profiling of the dynamic lytic transcriptome of BoHV-1 using two long-read sequencing (LRS) techniques, the Oxford Nanopore Technologies MinION, and the LoopSeq synthetic LRS methods, using multiple library preparation protocols. In this work, we annotated viral mRNAs and non-coding transcripts, and a large number of transcript isoforms, including transcription start and end sites, as well as splice variants of BoHV-1. Our analysis demonstrated an extremely complex pattern of transcriptional overlaps.
format	article
author	Norbert Moldován Gábor Torma Gábor Gulyás Ákos Hornyák Zoltán Zádori Victoria A. Jefferson Zsolt Csabai Miklós Boldogkői Dóra Tombácz Florencia Meyer Zsolt Boldogkői
author_facet	Norbert Moldován Gábor Torma Gábor Gulyás Ákos Hornyák Zoltán Zádori Victoria A. Jefferson Zsolt Csabai Miklós Boldogkői Dóra Tombácz Florencia Meyer Zsolt Boldogkői
author_sort	Norbert Moldován
title	Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_short	Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_full	Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_fullStr	Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_full_unstemmed	Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
title_sort	time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/836965dfd649493381b1c70a976eb07c
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Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing

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