Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.

<h4>Background</h4>The clinic therapeutic effect of resveratrol is limited due to its low oral bioavailability. Piceid, a precursor of resveratrol, is the most abundant form of resveratrol in nature. A number of studies have hypothesized that piceid may have the same bioactivities like t...

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Autores principales:	Dan Su, Ying Cheng, Miao Liu, Daozhou Liu, Han Cui, Bangle Zhang, Siyuan Zhou, Tiehong Yang, Qibing Mei
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83716a19ec6a4497bd8232b38c99c5e6
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spelling	oai:doaj.org-article:83716a19ec6a4497bd8232b38c99c5e62021-11-18T08:01:09ZComparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.1932-620310.1371/journal.pone.0054505https://doaj.org/article/83716a19ec6a4497bd8232b38c99c5e62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23342161/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The clinic therapeutic effect of resveratrol is limited due to its low oral bioavailability. Piceid, a precursor of resveratrol, is the most abundant form of resveratrol in nature. A number of studies have hypothesized that piceid may have the same bioactivities like those of resveratrol. The aim of this work is to compare piceid with resveratrol in antioxidation and antiproliferation activities in vitro.<h4>Methods</h4>The antioxidative effects of resveratrol and piceid were evaluated by phenanthroline-Fe²⁺ method and H₂O₂-induced oxidative injury cell model. The antiproliferation effects were determined by MTT method in human liver tumor HepG2 cells, human breast cancer MDA-MB-231 cells and MCF-7 cells. The effects of resveratrol and piceid on the cell cycle and the apoptosis were evaluated by flow cytometry. Additionally, the uptake profiles of resveratrol and piceid in cancer cells were observed using fluorescence microscopy and clarified by LC-MS/MS.<h4>Conclusion</h4>Piceid exhibited higher scavenging activity against hydroxyl radicals than resveratrol in vitro. Resveratrol showed a significant protective effect against H₂O₂-induced cell damage. What is more, resveratrol had biphasic effects on tumor cells. Resveratrol and piceid only showed significant cytotoxicity on tumor cells at high concentration (≥50 µmol/L), while low concentration of resveratrol (<30 µmol/L) increased the cell viability. The principal effect of resveratrol and piceid on the viability of tumor cells was caused by the cell cycle arrest, while the effect on apoptosis was relatively minor. The reason that piceid showed lower biological activity than resveratrol at the same concentration was probably because piceid was more difficult in being uptaken by cells.Dan SuYing ChengMiao LiuDaozhou LiuHan CuiBangle ZhangSiyuan ZhouTiehong YangQibing MeiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e54505 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan Su Ying Cheng Miao Liu Daozhou Liu Han Cui Bangle Zhang Siyuan Zhou Tiehong Yang Qibing Mei Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
description	<h4>Background</h4>The clinic therapeutic effect of resveratrol is limited due to its low oral bioavailability. Piceid, a precursor of resveratrol, is the most abundant form of resveratrol in nature. A number of studies have hypothesized that piceid may have the same bioactivities like those of resveratrol. The aim of this work is to compare piceid with resveratrol in antioxidation and antiproliferation activities in vitro.<h4>Methods</h4>The antioxidative effects of resveratrol and piceid were evaluated by phenanthroline-Fe²⁺ method and H₂O₂-induced oxidative injury cell model. The antiproliferation effects were determined by MTT method in human liver tumor HepG2 cells, human breast cancer MDA-MB-231 cells and MCF-7 cells. The effects of resveratrol and piceid on the cell cycle and the apoptosis were evaluated by flow cytometry. Additionally, the uptake profiles of resveratrol and piceid in cancer cells were observed using fluorescence microscopy and clarified by LC-MS/MS.<h4>Conclusion</h4>Piceid exhibited higher scavenging activity against hydroxyl radicals than resveratrol in vitro. Resveratrol showed a significant protective effect against H₂O₂-induced cell damage. What is more, resveratrol had biphasic effects on tumor cells. Resveratrol and piceid only showed significant cytotoxicity on tumor cells at high concentration (≥50 µmol/L), while low concentration of resveratrol (<30 µmol/L) increased the cell viability. The principal effect of resveratrol and piceid on the viability of tumor cells was caused by the cell cycle arrest, while the effect on apoptosis was relatively minor. The reason that piceid showed lower biological activity than resveratrol at the same concentration was probably because piceid was more difficult in being uptaken by cells.
format	article
author	Dan Su Ying Cheng Miao Liu Daozhou Liu Han Cui Bangle Zhang Siyuan Zhou Tiehong Yang Qibing Mei
author_facet	Dan Su Ying Cheng Miao Liu Daozhou Liu Han Cui Bangle Zhang Siyuan Zhou Tiehong Yang Qibing Mei
author_sort	Dan Su
title	Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
title_short	Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
title_full	Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
title_fullStr	Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
title_full_unstemmed	Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
title_sort	comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/83716a19ec6a4497bd8232b38c99c5e6
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Comparision of piceid and resveratrol in antioxidation and antiproliferation activities in vitro.

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