Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children.
<h4>Background</h4>Advances in molecular diagnostics have implicated newly-discovered respiratory viruses in the pathogenesis of pneumonia. We aimed to determine the prevalence and clinical characteristics of human bocavirus (hBoV), human rhinovirus (hRV), polyomavirus-WU (WUPyV) and -KI...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/8373482ba46e4d05a0bcf700e420ee3a |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:8373482ba46e4d05a0bcf700e420ee3a |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:8373482ba46e4d05a0bcf700e420ee3a2021-11-18T08:34:21ZClinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children.1932-620310.1371/journal.pone.0086448https://doaj.org/article/8373482ba46e4d05a0bcf700e420ee3a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498274/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Advances in molecular diagnostics have implicated newly-discovered respiratory viruses in the pathogenesis of pneumonia. We aimed to determine the prevalence and clinical characteristics of human bocavirus (hBoV), human rhinovirus (hRV), polyomavirus-WU (WUPyV) and -KI (KIPyV) and human coronaviruses (CoV)-OC43, -NL63, -HKU1 and -229E among children hospitalized with lower respiratory tract infections (LRTI).<h4>Methods</h4>Multiplex real-time reverse-transcriptase polymerase chain reaction was undertaken on archived nasopharyngeal aspirates from HIV-infected and -uninfected children (<2 years age) hospitalized for LRTI, who had been previously investigated for respiratory syncytial virus, human metapneumovirus, parainfluenza I-III, adenovirus and influenza A/B.<h4>Results</h4>At least one of these viruses were identified in 274 (53.0%) of 517 and in 509 (54.0%) of 943 LRTI-episodes in HIV-infected and -uninfected children, respectively. Human rhinovirus was the most prevalent in HIV-infected (31.7%) and -uninfected children (32.0%), followed by CoV-OC43 (12.2%) and hBoV (9.5%) in HIV-infected; and by hBoV (13.3%) and WUPyV (11.9%) in HIV-uninfected children. Polyomavirus-KI (8.9% vs. 4.8%; p = 0.002) and CoV-OC43 (12.2% vs. 3.6%; p<0.001) were more prevalent in HIV-infected than -uninfected children. Combined with previously-tested viruses, respiratory viruses were identified in 60.9% of HIV-infected and 78.3% of HIV-uninfected children. The newly tested viruses were detected at high frequency in association with other respiratory viruses, including previously-investigated viruses (22.8% in HIV-infected and 28.5% in HIV-uninfected children).<h4>Conclusions</h4>We established that combined with previously-investigated viruses, at least one respiratory virus was identified in the majority of HIV-infected and HIV-uninfected children hospitalized for LRTI. The high frequency of viral co-infections illustrates the complexities in attributing causality to specific viruses in the aetiology of LRTI and may indicate a synergetic role of viral co-infections in the pathogenesis of childhood LRTI.Marta C NunesZachary KuschnerZelda RabedeRichard MadimabeNadia Van NiekerkJackie MoloiLocadiah KuwandaJohn W RossenKeith P KlugmanPeter V AdrianShabir A MadhiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e86448 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Marta C Nunes Zachary Kuschner Zelda Rabede Richard Madimabe Nadia Van Niekerk Jackie Moloi Locadiah Kuwanda John W Rossen Keith P Klugman Peter V Adrian Shabir A Madhi Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children. |
| description |
<h4>Background</h4>Advances in molecular diagnostics have implicated newly-discovered respiratory viruses in the pathogenesis of pneumonia. We aimed to determine the prevalence and clinical characteristics of human bocavirus (hBoV), human rhinovirus (hRV), polyomavirus-WU (WUPyV) and -KI (KIPyV) and human coronaviruses (CoV)-OC43, -NL63, -HKU1 and -229E among children hospitalized with lower respiratory tract infections (LRTI).<h4>Methods</h4>Multiplex real-time reverse-transcriptase polymerase chain reaction was undertaken on archived nasopharyngeal aspirates from HIV-infected and -uninfected children (<2 years age) hospitalized for LRTI, who had been previously investigated for respiratory syncytial virus, human metapneumovirus, parainfluenza I-III, adenovirus and influenza A/B.<h4>Results</h4>At least one of these viruses were identified in 274 (53.0%) of 517 and in 509 (54.0%) of 943 LRTI-episodes in HIV-infected and -uninfected children, respectively. Human rhinovirus was the most prevalent in HIV-infected (31.7%) and -uninfected children (32.0%), followed by CoV-OC43 (12.2%) and hBoV (9.5%) in HIV-infected; and by hBoV (13.3%) and WUPyV (11.9%) in HIV-uninfected children. Polyomavirus-KI (8.9% vs. 4.8%; p = 0.002) and CoV-OC43 (12.2% vs. 3.6%; p<0.001) were more prevalent in HIV-infected than -uninfected children. Combined with previously-tested viruses, respiratory viruses were identified in 60.9% of HIV-infected and 78.3% of HIV-uninfected children. The newly tested viruses were detected at high frequency in association with other respiratory viruses, including previously-investigated viruses (22.8% in HIV-infected and 28.5% in HIV-uninfected children).<h4>Conclusions</h4>We established that combined with previously-investigated viruses, at least one respiratory virus was identified in the majority of HIV-infected and HIV-uninfected children hospitalized for LRTI. The high frequency of viral co-infections illustrates the complexities in attributing causality to specific viruses in the aetiology of LRTI and may indicate a synergetic role of viral co-infections in the pathogenesis of childhood LRTI. |
| format |
article |
| author |
Marta C Nunes Zachary Kuschner Zelda Rabede Richard Madimabe Nadia Van Niekerk Jackie Moloi Locadiah Kuwanda John W Rossen Keith P Klugman Peter V Adrian Shabir A Madhi |
| author_facet |
Marta C Nunes Zachary Kuschner Zelda Rabede Richard Madimabe Nadia Van Niekerk Jackie Moloi Locadiah Kuwanda John W Rossen Keith P Klugman Peter V Adrian Shabir A Madhi |
| author_sort |
Marta C Nunes |
| title |
Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children. |
| title_short |
Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children. |
| title_full |
Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children. |
| title_fullStr |
Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children. |
| title_full_unstemmed |
Clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in HIV-infected and HIV-uninfected South African children. |
| title_sort |
clinical epidemiology of bocavirus, rhinovirus, two polyomaviruses and four coronaviruses in hiv-infected and hiv-uninfected south african children. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/8373482ba46e4d05a0bcf700e420ee3a |
| work_keys_str_mv |
AT martacnunes clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT zacharykuschner clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT zeldarabede clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT richardmadimabe clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT nadiavanniekerk clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT jackiemoloi clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT locadiahkuwanda clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT johnwrossen clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT keithpklugman clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT petervadrian clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren AT shabiramadhi clinicalepidemiologyofbocavirusrhinovirustwopolyomavirusesandfourcoronavirusesinhivinfectedandhivuninfectedsouthafricanchildren |
| _version_ |
1718421631616942080 |