Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells

Ming-jun Zhang,1 Sheng-nan Liu,1 Ge Xu,2 Ya-nan Guo,1 Jian-nan Fu,3 De-chun Zhang11Molecular Medicine and Tumor Research Center, Chongqing Medical University, 2Electron Microscopy Group, Department of Life Science, Chongqing Medical University, Chongqing, People's Republic of China; 3First...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang MJ, Liu SN, Xu G, Guo YN, Fu JN, Zhang DC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/83764f387b5d4af48d9a8c7dcec26658
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:83764f387b5d4af48d9a8c7dcec26658
record_format	dspace
spelling	oai:doaj.org-article:83764f387b5d4af48d9a8c7dcec266582021-12-02T06:01:09ZCytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells1178-2013https://doaj.org/article/83764f387b5d4af48d9a8c7dcec266582013-12-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-and-apoptosis-induced-by-nanobacteria-in-human-breast-can-a15404https://doaj.org/toc/1178-2013 Ming-jun Zhang,1 Sheng-nan Liu,1 Ge Xu,2 Ya-nan Guo,1 Jian-nan Fu,3 De-chun Zhang11Molecular Medicine and Tumor Research Center, Chongqing Medical University, 2Electron Microscopy Group, Department of Life Science, Chongqing Medical University, Chongqing, People's Republic of China; 3First People's Hospital of Jiulongpo District, Chongqing, People's Republic of ChinaBackground: The existing evidence that nanobacteria (NB) are closely associated with human disease is overwhelming. However, their potential toxicity against cancer cells has not yet been reported. The objective of this study was to investigate the cytotoxic effects of NB and nanohydroxyapatites (nHAPs) against human breast cancer cells and to elucidate the mechanisms of action underlying their cytotoxicity.Methodology/principal findings: NB were isolated from calcified placental tissue, and nHAPs were artificially synthesized. The viability of the MDA-MB-231 human breast cancer cell line was tested by using the Kit-8 cell counting kit assay. Apoptosis was examined by transmission electron microscopy and flow cytometry. The endocytosis of NB and nHAPs by MDA-MB-231 cells was initially confirmed by microscopy. Although both NB and nHAPs significantly decreased MDA-MB-231 cell viability and increased the population of apoptotic cells, NB were more potent than nHAPs. After 72 hours, NB also caused ultrastructural changes typical of apoptosis, such as chromatin condensation, nuclear fragmentation, nuclear dissolution, mitochondrial swelling, and the formation of apoptotic bodies.Conclusion/significance: In MDA-MB-231 human breast cancer cells, NB and nHAPs exerted cytotoxic effects that were associated with the induction of apoptosis. The effects exerted by NB were more potent than those induced by nHAPs. NB cytotoxicity probably emerged from toxic metabolites or protein components, rather than merely the hydroxyapatite shells. NB divided during culturing, and similar to cells undergoing binary fission, many NB particles were observed in culture by transmission electron microscopy, suggesting they are live microorganisms.Keywords: nanobacteria, nanohydroxyapatites, human breast cancer MDA-MB-231 cells, cytotoxicity, apoptosisZhang MJLiu SNXu GGuo YNFu JNZhang DCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 265-271 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang MJ Liu SN Xu G Guo YN Fu JN Zhang DC Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
description	Ming-jun Zhang,1 Sheng-nan Liu,1 Ge Xu,2 Ya-nan Guo,1 Jian-nan Fu,3 De-chun Zhang11Molecular Medicine and Tumor Research Center, Chongqing Medical University, 2Electron Microscopy Group, Department of Life Science, Chongqing Medical University, Chongqing, People's Republic of China; 3First People's Hospital of Jiulongpo District, Chongqing, People's Republic of ChinaBackground: The existing evidence that nanobacteria (NB) are closely associated with human disease is overwhelming. However, their potential toxicity against cancer cells has not yet been reported. The objective of this study was to investigate the cytotoxic effects of NB and nanohydroxyapatites (nHAPs) against human breast cancer cells and to elucidate the mechanisms of action underlying their cytotoxicity.Methodology/principal findings: NB were isolated from calcified placental tissue, and nHAPs were artificially synthesized. The viability of the MDA-MB-231 human breast cancer cell line was tested by using the Kit-8 cell counting kit assay. Apoptosis was examined by transmission electron microscopy and flow cytometry. The endocytosis of NB and nHAPs by MDA-MB-231 cells was initially confirmed by microscopy. Although both NB and nHAPs significantly decreased MDA-MB-231 cell viability and increased the population of apoptotic cells, NB were more potent than nHAPs. After 72 hours, NB also caused ultrastructural changes typical of apoptosis, such as chromatin condensation, nuclear fragmentation, nuclear dissolution, mitochondrial swelling, and the formation of apoptotic bodies.Conclusion/significance: In MDA-MB-231 human breast cancer cells, NB and nHAPs exerted cytotoxic effects that were associated with the induction of apoptosis. The effects exerted by NB were more potent than those induced by nHAPs. NB cytotoxicity probably emerged from toxic metabolites or protein components, rather than merely the hydroxyapatite shells. NB divided during culturing, and similar to cells undergoing binary fission, many NB particles were observed in culture by transmission electron microscopy, suggesting they are live microorganisms.Keywords: nanobacteria, nanohydroxyapatites, human breast cancer MDA-MB-231 cells, cytotoxicity, apoptosis
format	article
author	Zhang MJ Liu SN Xu G Guo YN Fu JN Zhang DC
author_facet	Zhang MJ Liu SN Xu G Guo YN Fu JN Zhang DC
author_sort	Zhang MJ
title	Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
title_short	Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
title_full	Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
title_fullStr	Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
title_full_unstemmed	Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
title_sort	cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/83764f387b5d4af48d9a8c7dcec26658
work_keys_str_mv	AT zhangmj cytotoxicityandapoptosisinducedbynanobacteriainhumanbreastcancercells AT liusn cytotoxicityandapoptosisinducedbynanobacteriainhumanbreastcancercells AT xug cytotoxicityandapoptosisinducedbynanobacteriainhumanbreastcancercells AT guoyn cytotoxicityandapoptosisinducedbynanobacteriainhumanbreastcancercells AT fujn cytotoxicityandapoptosisinducedbynanobacteriainhumanbreastcancercells AT zhangdc cytotoxicityandapoptosisinducedbynanobacteriainhumanbreastcancercells
_version_	1718400118789505024

Cytotoxicity and apoptosis induced by nanobacteria in human breast cancer cells

Ejemplares similares