Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV

Kaposi’s sarcoma-associated herpesvirus (KSHV) is a cancer-related virus which engages in two forms of infection: latent and lytic. Latent infection allows the virus to establish long-term persistent infection, whereas the lytic cycle is needed for the maintenance of the viral reservoir and for viru...

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Autores principales:	Chen Gam ze Letova, Inna Kalt, Meir Shamay, Ronit Sarid
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Kaposi’s sarcoma-associated herpesvirus (KSHV) superinfection primary infection latency-associated nuclear antigen 1 (LANA-1) latent infection Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8377516422ee408097867aef96eee898
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spelling	oai:doaj.org-article:8377516422ee408097867aef96eee8982021-11-11T17:24:35ZLatently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV10.3390/ijms2221119941422-00671661-6596https://doaj.org/article/8377516422ee408097867aef96eee8982021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11994https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Kaposi’s sarcoma-associated herpesvirus (KSHV) is a cancer-related virus which engages in two forms of infection: latent and lytic. Latent infection allows the virus to establish long-term persistent infection, whereas the lytic cycle is needed for the maintenance of the viral reservoir and for virus spread. By using recombinant KSHV viruses encoding mNeonGreen and mCherry fluorescent proteins, we show that various cell types that are latently-infected with KSHV can be superinfected, and that the new incoming viruses establish latent infection. Moreover, we show that latency establishment is enhanced in superinfected cells compared to primary infected ones. Further analysis revealed that cells that ectopically express the major latency protein of KSHV, LANA-1, prior to and during infection exhibit enhanced establishment of latency, but not cells expressing LANA-1 fragments. This observation supports the notion that the expression level of LANA-1 following infection determines the efficiency of latency establishment and avoids loss of viral genomes. These findings imply that a host can be infected with more than a single viral genome and that superinfection may support the maintenance of long-term latency.Chen Gam ze LetovaInna KaltMeir ShamayRonit SaridMDPI AGarticleKaposi’s sarcoma-associated herpesvirus (KSHV)superinfectionprimary infectionlatency-associated nuclear antigen 1 (LANA-1)latent infectionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11994, p 11994 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Kaposi’s sarcoma-associated herpesvirus (KSHV) superinfection primary infection latency-associated nuclear antigen 1 (LANA-1) latent infection Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Kaposi’s sarcoma-associated herpesvirus (KSHV) superinfection primary infection latency-associated nuclear antigen 1 (LANA-1) latent infection Biology (General) QH301-705.5 Chemistry QD1-999 Chen Gam ze Letova Inna Kalt Meir Shamay Ronit Sarid Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV
description	Kaposi’s sarcoma-associated herpesvirus (KSHV) is a cancer-related virus which engages in two forms of infection: latent and lytic. Latent infection allows the virus to establish long-term persistent infection, whereas the lytic cycle is needed for the maintenance of the viral reservoir and for virus spread. By using recombinant KSHV viruses encoding mNeonGreen and mCherry fluorescent proteins, we show that various cell types that are latently-infected with KSHV can be superinfected, and that the new incoming viruses establish latent infection. Moreover, we show that latency establishment is enhanced in superinfected cells compared to primary infected ones. Further analysis revealed that cells that ectopically express the major latency protein of KSHV, LANA-1, prior to and during infection exhibit enhanced establishment of latency, but not cells expressing LANA-1 fragments. This observation supports the notion that the expression level of LANA-1 following infection determines the efficiency of latency establishment and avoids loss of viral genomes. These findings imply that a host can be infected with more than a single viral genome and that superinfection may support the maintenance of long-term latency.
format	article
author	Chen Gam ze Letova Inna Kalt Meir Shamay Ronit Sarid
author_facet	Chen Gam ze Letova Inna Kalt Meir Shamay Ronit Sarid
author_sort	Chen Gam ze Letova
title	Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV
title_short	Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV
title_full	Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV
title_fullStr	Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV
title_full_unstemmed	Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV
title_sort	latently kshv-infected cells promote further establishment of latency upon superinfection with kshv
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8377516422ee408097867aef96eee898
work_keys_str_mv	AT chengamzeletova latentlykshvinfectedcellspromotefurtherestablishmentoflatencyuponsuperinfectionwithkshv AT innakalt latentlykshvinfectedcellspromotefurtherestablishmentoflatencyuponsuperinfectionwithkshv AT meirshamay latentlykshvinfectedcellspromotefurtherestablishmentoflatencyuponsuperinfectionwithkshv AT ronitsarid latentlykshvinfectedcellspromotefurtherestablishmentoflatencyuponsuperinfectionwithkshv
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Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV

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