Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells

Natalia L Chaves,1 Irina Estrela-Lopis,2 Julia Böttner,2 Cláudio AP Lopes,1 Bruna C Guido,1 Aparecido R de Sousa,3 Sônia N Báo1 1Institute of Biological Sciences, Department of Cell Biology, University of Brasília (UnB), Brasília, Brazi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chaves NL, Estrela-Lopis I, Böttner J, Lopes CAP, Guido BC, de Souza AR, Báo SN
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Maghemite nanomaterials cells uptake endocytosis. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8379ee08656c43b887292574eb22e4c1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8379ee08656c43b887292574eb22e4c1
record_format	dspace
spelling	oai:doaj.org-article:8379ee08656c43b887292574eb22e4c12021-12-02T03:11:31ZExploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells1178-2013https://doaj.org/article/8379ee08656c43b887292574eb22e4c12017-08-01T00:00:00Zhttps://www.dovepress.com/exploring-cellular-uptake-of-iron-oxide-nanoparticles-associated-with--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Natalia L Chaves,1 Irina Estrela-Lopis,2 Julia Böttner,2 Cláudio AP Lopes,1 Bruna C Guido,1 Aparecido R de Sousa,3 Sônia N Báo1 1Institute of Biological Sciences, Department of Cell Biology, University of Brasília (UnB), Brasília, Brazil; 2Institute of Biophysics and Medical Physics, University of Leipzig, Leipzig, Germany; 3Institute of Chemistry, Federal University of Goiás, Goiânia, Brazil Abstract: Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite–rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specificity of the cytotoxic action. However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated. This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line. MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution. Moreover, this process occurred through a clathrin-dependent endocytosis pathway with different basal expression levels of this protein in the cell lines tested. Keywords: maghemite, nanomaterials, cells uptake, endocytosisChaves NLEstrela-Lopis IBöttner JLopes CAPGuido BCde Souza ARBáo SNDove Medical PressarticleMaghemitenanomaterialscells uptakeendocytosis.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5511-5523 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Maghemite nanomaterials cells uptake endocytosis. Medicine (General) R5-920
spellingShingle	Maghemite nanomaterials cells uptake endocytosis. Medicine (General) R5-920 Chaves NL Estrela-Lopis I Böttner J Lopes CAP Guido BC de Souza AR Báo SN Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
description	Natalia L Chaves,1 Irina Estrela-Lopis,2 Julia Böttner,2 Cláudio AP Lopes,1 Bruna C Guido,1 Aparecido R de Sousa,3 Sônia N Báo1 1Institute of Biological Sciences, Department of Cell Biology, University of Brasília (UnB), Brasília, Brazil; 2Institute of Biophysics and Medical Physics, University of Leipzig, Leipzig, Germany; 3Institute of Chemistry, Federal University of Goiás, Goiânia, Brazil Abstract: Nanocarriers have the potential to improve the therapeutic index of currently available drugs by improving their efficacy and achieving therapeutic steady-state levels over an extended period. The association of maghemite–rhodium citrate (MRC) nanoparticles (NPs) has the potential to increase specificity of the cytotoxic action. However, the interaction of these NPs with cells, their uptake mechanism, and subcellular localization need to be elucidated. This work evaluates the uptake mechanism of MRC NPs in metastatic and nonmetastatic breast cancer-cell models, comparing them to a nontumor cell line. MRC NPs uptake in breast cancer cells was more effective than in normal cells, with regard to both the amount of internalized material and the achievement of more strategic intracellular distribution. Moreover, this process occurred through a clathrin-dependent endocytosis pathway with different basal expression levels of this protein in the cell lines tested. Keywords: maghemite, nanomaterials, cells uptake, endocytosis
format	article
author	Chaves NL Estrela-Lopis I Böttner J Lopes CAP Guido BC de Souza AR Báo SN
author_facet	Chaves NL Estrela-Lopis I Böttner J Lopes CAP Guido BC de Souza AR Báo SN
author_sort	Chaves NL
title	Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_short	Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_full	Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_fullStr	Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_full_unstemmed	Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
title_sort	exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8379ee08656c43b887292574eb22e4c1
work_keys_str_mv	AT chavesnl exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells AT estrelalopisi exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells AT bottnerj exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells AT lopescap exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells AT guidobc exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells AT desouzaar exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells AT baosn exploringcellularuptakeofironoxidenanoparticlesassociatedwithrhodiumcitrateinbreastcancercells
_version_	1718401927986806784

Exploring cellular uptake of iron oxide nanoparticles associated with rhodium citrate in breast cancer cells

Ejemplares similares