The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates

ABSTRACT Kingella kingae is an encapsulated Gram-negative bacterium and an important etiology of osteoarticular infections in young children. A recent study examining a diverse collection of carrier and invasive K. kingae isolates from Israel revealed four distinct polysaccharide capsule types. In t...

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Autores principales:	Eric A. Porsch, Kimberly F. Starr, Pablo Yagupsky, Joseph W. St. Geme
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Kingella kingae PCR capsule typing clinical isolates polysaccharide capsule Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8388723c3c9d4e93a436e0952fbcca42
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spelling	oai:doaj.org-article:8388723c3c9d4e93a436e0952fbcca422021-11-15T15:21:46ZThe Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates10.1128/mSphere.00060-172379-5042https://doaj.org/article/8388723c3c9d4e93a436e0952fbcca422017-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00060-17https://doaj.org/toc/2379-5042ABSTRACT Kingella kingae is an encapsulated Gram-negative bacterium and an important etiology of osteoarticular infections in young children. A recent study examining a diverse collection of carrier and invasive K. kingae isolates from Israel revealed four distinct polysaccharide capsule types. In this study, to obtain a global view of K. kingae capsule type diversity, we examined an international collection of isolates using a multiplex PCR approach. The collection contained all four previously identified capsule types and no new capsule types. Over 95% of invasive isolates in the collection were type a or type b, similar to the findings in Israel. These results suggest that the type a and type b polysaccharide capsules may have enhanced pathogenic properties or may mark clonal groups of strains with specific virulence genes. In addition, they raise the possibility that a vaccine containing the type a and type b capsules might be an effective approach to preventing K. kingae disease. IMPORTANCE Kingella kingae has emerged as a significant cause of septic arthritis, osteomyelitis, and bacteremia in young children. A recent study examining a diverse collection of K. kingae isolates from Israel revealed four different polysaccharide capsule types in this species, designated types a to d. To determine the global distribution of K. kingae capsule types, we assembled and capsule typed an international collection of K. kingae isolates. The findings reported here show that the type a and type b capsules represent >95% of the invasive isolates, similar to the Israeli isolate collection, suggesting that a polysaccharide-based vaccine targeting these two capsules could be an attractive approach to prevent K. kingae disease.Eric A. PorschKimberly F. StarrPablo YagupskyJoseph W. St. GemeAmerican Society for MicrobiologyarticleKingella kingaePCRcapsule typingclinical isolatespolysaccharide capsuleMicrobiologyQR1-502ENmSphere, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Kingella kingae PCR capsule typing clinical isolates polysaccharide capsule Microbiology QR1-502
spellingShingle	Kingella kingae PCR capsule typing clinical isolates polysaccharide capsule Microbiology QR1-502 Eric A. Porsch Kimberly F. Starr Pablo Yagupsky Joseph W. St. Geme The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates
description	ABSTRACT Kingella kingae is an encapsulated Gram-negative bacterium and an important etiology of osteoarticular infections in young children. A recent study examining a diverse collection of carrier and invasive K. kingae isolates from Israel revealed four distinct polysaccharide capsule types. In this study, to obtain a global view of K. kingae capsule type diversity, we examined an international collection of isolates using a multiplex PCR approach. The collection contained all four previously identified capsule types and no new capsule types. Over 95% of invasive isolates in the collection were type a or type b, similar to the findings in Israel. These results suggest that the type a and type b polysaccharide capsules may have enhanced pathogenic properties or may mark clonal groups of strains with specific virulence genes. In addition, they raise the possibility that a vaccine containing the type a and type b capsules might be an effective approach to preventing K. kingae disease. IMPORTANCE Kingella kingae has emerged as a significant cause of septic arthritis, osteomyelitis, and bacteremia in young children. A recent study examining a diverse collection of K. kingae isolates from Israel revealed four different polysaccharide capsule types in this species, designated types a to d. To determine the global distribution of K. kingae capsule types, we assembled and capsule typed an international collection of K. kingae isolates. The findings reported here show that the type a and type b capsules represent >95% of the invasive isolates, similar to the Israeli isolate collection, suggesting that a polysaccharide-based vaccine targeting these two capsules could be an attractive approach to prevent K. kingae disease.
format	article
author	Eric A. Porsch Kimberly F. Starr Pablo Yagupsky Joseph W. St. Geme
author_facet	Eric A. Porsch Kimberly F. Starr Pablo Yagupsky Joseph W. St. Geme
author_sort	Eric A. Porsch
title	The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates
title_short	The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates
title_full	The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates
title_fullStr	The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates
title_full_unstemmed	The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates
title_sort	type a and type b polysaccharide capsules predominate in an international collection of invasive <named-content content-type="genus-species">kingella kingae</named-content> isolates
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/8388723c3c9d4e93a436e0952fbcca42
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The Type a and Type b Polysaccharide Capsules Predominate in an International Collection of Invasive <named-content content-type="genus-species">Kingella kingae</named-content> Isolates

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