Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.

Organic isothiocyanates (ITCs) are allelochemicals produced by plants in order to combat insects and other herbivores. The compounds are toxic electrophiles that can be inactivated and conjugated with intracellular glutathione in reactions catalyzed by glutathione transferases (GSTs). The Drosophila...

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Autores principales:	Aslam M A Mazari, Olle Dahlberg, Bengt Mannervik, Mattias Mannervik
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/838ba7f23f634d49850a438d69d96b98
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spelling	oai:doaj.org-article:838ba7f23f634d49850a438d69d96b982021-11-25T05:56:18ZOverexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.1932-620310.1371/journal.pone.0110103https://doaj.org/article/838ba7f23f634d49850a438d69d96b982014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110103https://doaj.org/toc/1932-6203Organic isothiocyanates (ITCs) are allelochemicals produced by plants in order to combat insects and other herbivores. The compounds are toxic electrophiles that can be inactivated and conjugated with intracellular glutathione in reactions catalyzed by glutathione transferases (GSTs). The Drosophila melanogaster GSTE7 was heterologously expressed in Escherichia coli and purified for functional studies. The enzyme showed high catalytic activity with various isothiocyanates including phenethyl isothiocyanate (PEITC) and allyl isothiocyanate (AITC), which in millimolar dietary concentrations conferred toxicity to adult D. melanogaster leading to death or a shortened life-span of the flies. In situ hybridization revealed a maternal contribution of GSTE7 transcripts to embryos, and strongest zygotic expression in the digestive tract. Transgenesis involving the GSTE7 gene controlled by an actin promoter produced viable flies expressing the GSTE7 transcript ubiquitously. Transgenic females show a significantly increased survival when subjected to the same PEITC treatment as the wild-type flies. By contrast, transgenic male flies show a significantly lower survival rate. Oviposition activity was enhanced in transgenic flies. The effect was significant in transgenic females reared in the absence of ITCs as well as in the presence of 0.15 mM PEITC or 1 mM AITC. Thus the GSTE7 transgene elicits responses to exposure to ITC allelochemicals which differentially affect life-span and fecundity of male and female flies.Aslam M A MazariOlle DahlbergBengt MannervikMattias MannervikPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110103 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aslam M A Mazari Olle Dahlberg Bengt Mannervik Mattias Mannervik Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
description	Organic isothiocyanates (ITCs) are allelochemicals produced by plants in order to combat insects and other herbivores. The compounds are toxic electrophiles that can be inactivated and conjugated with intracellular glutathione in reactions catalyzed by glutathione transferases (GSTs). The Drosophila melanogaster GSTE7 was heterologously expressed in Escherichia coli and purified for functional studies. The enzyme showed high catalytic activity with various isothiocyanates including phenethyl isothiocyanate (PEITC) and allyl isothiocyanate (AITC), which in millimolar dietary concentrations conferred toxicity to adult D. melanogaster leading to death or a shortened life-span of the flies. In situ hybridization revealed a maternal contribution of GSTE7 transcripts to embryos, and strongest zygotic expression in the digestive tract. Transgenesis involving the GSTE7 gene controlled by an actin promoter produced viable flies expressing the GSTE7 transcript ubiquitously. Transgenic females show a significantly increased survival when subjected to the same PEITC treatment as the wild-type flies. By contrast, transgenic male flies show a significantly lower survival rate. Oviposition activity was enhanced in transgenic flies. The effect was significant in transgenic females reared in the absence of ITCs as well as in the presence of 0.15 mM PEITC or 1 mM AITC. Thus the GSTE7 transgene elicits responses to exposure to ITC allelochemicals which differentially affect life-span and fecundity of male and female flies.
format	article
author	Aslam M A Mazari Olle Dahlberg Bengt Mannervik Mattias Mannervik
author_facet	Aslam M A Mazari Olle Dahlberg Bengt Mannervik Mattias Mannervik
author_sort	Aslam M A Mazari
title	Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
title_short	Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
title_full	Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
title_fullStr	Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
title_full_unstemmed	Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
title_sort	overexpression of glutathione transferase e7 in drosophila differentially impacts toxicity of organic isothiocyanates in males and females.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/838ba7f23f634d49850a438d69d96b98
work_keys_str_mv	AT aslammamazari overexpressionofglutathionetransferasee7indrosophiladifferentiallyimpactstoxicityoforganicisothiocyanatesinmalesandfemales AT olledahlberg overexpressionofglutathionetransferasee7indrosophiladifferentiallyimpactstoxicityoforganicisothiocyanatesinmalesandfemales AT bengtmannervik overexpressionofglutathionetransferasee7indrosophiladifferentiallyimpactstoxicityoforganicisothiocyanatesinmalesandfemales AT mattiasmannervik overexpressionofglutathionetransferasee7indrosophiladifferentiallyimpactstoxicityoforganicisothiocyanatesinmalesandfemales
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Overexpression of glutathione transferase E7 in Drosophila differentially impacts toxicity of organic isothiocyanates in males and females.

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