Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages

Christina L Ross,1,2 Benjamin S Harrison2 1Akamai University, Department of Energy Medicine, Hilo, HI, USA; 2Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Health, Winston-Salem, NC, USA Abstract: In the treatment of bacterial infections, antibiotics have proven to be very effe...

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Autores principales:	Ross CL, Harrison BS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8390977412224bf2a5a0005002b859b5
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spelling	oai:doaj.org-article:8390977412224bf2a5a0005002b859b52021-12-02T02:09:14ZEffect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages1178-7031https://doaj.org/article/8390977412224bf2a5a0005002b859b52013-03-01T00:00:00Zhttp://www.dovepress.com/effect-of-pulsed-electromagnetic-field-on-inflammatory-pathway-markers-a12441https://doaj.org/toc/1178-7031Christina L Ross,1,2 Benjamin S Harrison2 1Akamai University, Department of Energy Medicine, Hilo, HI, USA; 2Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Health, Winston-Salem, NC, USA Abstract: In the treatment of bacterial infections, antibiotics have proven to be very effective, but the way in which antibiotics are dosed can create a lag time between the administration of the drug and its absorption at the site of insult. The time it takes an antibiotic to reach therapeutic levels can often be significantly increased if the vascular system is compromized. Bacteria can multiply pending the delivery of the drug, therefore, developing treatments that can inhibit the inflammatory response while waiting for antibiotics to take effect could help prevent medical conditions such as septic shock. The aim of this study was to examine the effect of a pulsed electromagnetic field on the production of inflammatory markers tumor necrosis factor (TNF), transcription factor nuclear factor kappa B (NFkB), and the expression of the A20 (tumor necrosis factor-alpha-induced protein 3), in an inflamed-cell model. Lipopolysaccharide-challenged cells were exposed to a pulsed electromagnetic field at various frequencies in order to determine which, if any, frequency would affect the TNF-NFkB-A20 inflammatory response pathway. Our study revealed that cells continuously exposed to a pulsed electromagnetic field at 5 Hz demonstrated significant changes in the downregulation of TNF-&alpha; and NFkB and also showed a trend in the down regulation of A20, as compared with controls. This treatment could be beneficial in modulating the immune response, in the presence of infection. Keyword: TNFAIP3, pulsed electromagnetic field, macrophages, TNF, NFkBRoss CLHarrison BSDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2013, Iss default, Pp 45-51 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Ross CL Harrison BS Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
description	Christina L Ross,1,2 Benjamin S Harrison2 1Akamai University, Department of Energy Medicine, Hilo, HI, USA; 2Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Health, Winston-Salem, NC, USA Abstract: In the treatment of bacterial infections, antibiotics have proven to be very effective, but the way in which antibiotics are dosed can create a lag time between the administration of the drug and its absorption at the site of insult. The time it takes an antibiotic to reach therapeutic levels can often be significantly increased if the vascular system is compromized. Bacteria can multiply pending the delivery of the drug, therefore, developing treatments that can inhibit the inflammatory response while waiting for antibiotics to take effect could help prevent medical conditions such as septic shock. The aim of this study was to examine the effect of a pulsed electromagnetic field on the production of inflammatory markers tumor necrosis factor (TNF), transcription factor nuclear factor kappa B (NFkB), and the expression of the A20 (tumor necrosis factor-alpha-induced protein 3), in an inflamed-cell model. Lipopolysaccharide-challenged cells were exposed to a pulsed electromagnetic field at various frequencies in order to determine which, if any, frequency would affect the TNF-NFkB-A20 inflammatory response pathway. Our study revealed that cells continuously exposed to a pulsed electromagnetic field at 5 Hz demonstrated significant changes in the downregulation of TNF-&alpha; and NFkB and also showed a trend in the down regulation of A20, as compared with controls. This treatment could be beneficial in modulating the immune response, in the presence of infection. Keyword: TNFAIP3, pulsed electromagnetic field, macrophages, TNF, NFkB
format	article
author	Ross CL Harrison BS
author_facet	Ross CL Harrison BS
author_sort	Ross CL
title	Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
title_short	Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
title_full	Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
title_fullStr	Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
title_full_unstemmed	Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages
title_sort	effect of pulsed electromagnetic field on inflammatory pathway markers in raw 264.7 murine macrophages
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/8390977412224bf2a5a0005002b859b5
work_keys_str_mv	AT rosscl effectofpulsedelectromagneticfieldoninflammatorypathwaymarkersinraw2647murinemacrophages AT harrisonbs effectofpulsedelectromagneticfieldoninflammatorypathwaymarkersinraw2647murinemacrophages
_version_	1718402659124248576

Effect of pulsed electromagnetic field on inflammatory pathway markers in RAW 264.7 murine macrophages

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