Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.

The high prevalence of morbidity and mortality from bacterial infections, together with the growing threat of antibacterial resistance, necessitated the development of alternative new drugs from traditional medicine. In Ethiopia, Impatiens tinctoria A. Rich has been traditionally used for the treatm...

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Autores principales:	Sileshi Degu, Abiy Abebe, Negero Gemeda, Adane Bitew
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8390ef58c9504313bb652a606174ad35
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spelling	oai:doaj.org-article:8390ef58c9504313bb652a606174ad352021-12-02T20:18:22ZEvaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.1932-620310.1371/journal.pone.0255932https://doaj.org/article/8390ef58c9504313bb652a606174ad352021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255932https://doaj.org/toc/1932-6203The high prevalence of morbidity and mortality from bacterial infections, together with the growing threat of antibacterial resistance, necessitated the development of alternative new drugs from traditional medicine. In Ethiopia, Impatiens tinctoria A. Rich has been traditionally used for the treatment of fungal infections such as ringworms that cause tinea pedis and it have also different medical values. Scientific information on its biological activity against a broad range of bacteria and safety data is scant, compared to its folklore data. In this study, we evaluated antibacterial activities and acute oral toxicity of aqueous, ethanol and ethyl acetate root extracts of Impatiens tinctoria A. Rich. Aqueous, ethanol and ethyl acetate extracts of the plant were evaluated using agar hole diffusion and agar dilution methods. Biological activities of the plant extracts were expressed as a zone of inhibition diameter, minimum inhibitory concentration (mg/ml), and minimum bactericidal concentration (mg/ml). The safety studies were performed by oral acute toxicity study according to the organization of economic cooperation and development test Guidelines 420.Gram-positive bacteria were more susceptible to the extracts compared to gram-negative bacteria, especially against S. aureus and S. epidermidis, which are commonly found in the skin. Ethyl acetate extract was more potent than ethanol and aqueous extracts. The 50% lethal dose (LD50) of tested mice was above 9600 mg/kg. This study provides a scientific basis for the antibacterial activity of the root extracts of I. tinctoria A. Rich, where, the ethyl acetate extract showed the most promising activity. Therefore, the antibacterial potential and practical non-toxicity of the study plant extracts suggested the possibility of using it for the development of antimicrobial drugs by further studying the plant in different directions.Sileshi DeguAbiy AbebeNegero GemedaAdane BitewPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255932 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sileshi Degu Abiy Abebe Negero Gemeda Adane Bitew Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.
description	The high prevalence of morbidity and mortality from bacterial infections, together with the growing threat of antibacterial resistance, necessitated the development of alternative new drugs from traditional medicine. In Ethiopia, Impatiens tinctoria A. Rich has been traditionally used for the treatment of fungal infections such as ringworms that cause tinea pedis and it have also different medical values. Scientific information on its biological activity against a broad range of bacteria and safety data is scant, compared to its folklore data. In this study, we evaluated antibacterial activities and acute oral toxicity of aqueous, ethanol and ethyl acetate root extracts of Impatiens tinctoria A. Rich. Aqueous, ethanol and ethyl acetate extracts of the plant were evaluated using agar hole diffusion and agar dilution methods. Biological activities of the plant extracts were expressed as a zone of inhibition diameter, minimum inhibitory concentration (mg/ml), and minimum bactericidal concentration (mg/ml). The safety studies were performed by oral acute toxicity study according to the organization of economic cooperation and development test Guidelines 420.Gram-positive bacteria were more susceptible to the extracts compared to gram-negative bacteria, especially against S. aureus and S. epidermidis, which are commonly found in the skin. Ethyl acetate extract was more potent than ethanol and aqueous extracts. The 50% lethal dose (LD50) of tested mice was above 9600 mg/kg. This study provides a scientific basis for the antibacterial activity of the root extracts of I. tinctoria A. Rich, where, the ethyl acetate extract showed the most promising activity. Therefore, the antibacterial potential and practical non-toxicity of the study plant extracts suggested the possibility of using it for the development of antimicrobial drugs by further studying the plant in different directions.
format	article
author	Sileshi Degu Abiy Abebe Negero Gemeda Adane Bitew
author_facet	Sileshi Degu Abiy Abebe Negero Gemeda Adane Bitew
author_sort	Sileshi Degu
title	Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.
title_short	Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.
title_full	Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.
title_fullStr	Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.
title_full_unstemmed	Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.
title_sort	evaluation of antibacterial and acute oral toxicity of impatiens tinctoria a. rich root extracts.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8390ef58c9504313bb652a606174ad35
work_keys_str_mv	AT sileshidegu evaluationofantibacterialandacuteoraltoxicityofimpatienstinctoriaarichrootextracts AT abiyabebe evaluationofantibacterialandacuteoraltoxicityofimpatienstinctoriaarichrootextracts AT negerogemeda evaluationofantibacterialandacuteoraltoxicityofimpatienstinctoriaarichrootextracts AT adanebitew evaluationofantibacterialandacuteoraltoxicityofimpatienstinctoriaarichrootextracts
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Evaluation of antibacterial and acute oral toxicity of Impatiens tinctoria A. Rich root extracts.

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