Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

Field pea (Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant va...

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Autores principales:	Shaoming Huang, Krishna K. Gali, Reddy V. B. Lachagari, Navajeet Chakravartty, Rosalind A. Bueckert, Bunyamin Tar’an, Thomas D. Warkentin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/839db5162082416cb237412cdac4f05c
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spelling	oai:doaj.org-article:839db5162082416cb237412cdac4f05c2021-11-11T07:14:39ZIdentification of heat responsive genes in pea stipules and anthers through transcriptional profiling1932-6203https://doaj.org/article/839db5162082416cb237412cdac4f05c2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568175/?tool=EBIhttps://doaj.org/toc/1932-6203Field pea (Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant variety based on its similar field performance as PR11-2. This study aimed to characterize the differential transcription. Plants of these three varieties were stressed for 3 h at 38°C prior to self-pollination, and RNAs from heat stressed anthers and stipules on the same flowering node were extracted and sequenced via the Illumina NovaSeq platform for the characterization of heat responsive genes. In silico results were further validated by qPCR assay. Differentially expressed genes (DEGs) were identified at log2 \|fold change (FC)\| ≥ 2 between high temperature and control temperature, the three varieties shared 588 DEGs which were up-regulated and 220 genes which were down-regulated in anthers when subjected to heat treatment. In stipules, 879 DEGs (463/416 upregulation/downregulation) were consistent among varieties. The above heat-induced genes of the two plant organs were related to several biological processes i.e., response to heat, protein folding and DNA templated transcription. Ten gene ontology (GO) terms were over-represented in the consistently down-regulated DEGs of the two organs, and these terms were mainly related to cell wall macromolecule metabolism, lipid transport, lipid localization, and lipid metabolic processes. GO enrichment analysis on distinct DEGs of individual pea varieties suggested that heat affected biological processes were dynamic, and variety distinct responses provide insight into molecular mechanisms of heat-tolerance response. Several biological processes, e.g., cellular response to DNA damage stimulus in stipule, electron transport chain in anther that were only observed in heat induced PR11-2 and CDC Amarillo, and their relevance to field pea heat tolerance is worth further validation.Shaoming HuangKrishna K. GaliReddy V. B. LachagariNavajeet ChakravarttyRosalind A. BueckertBunyamin Tar’anThomas D. WarkentinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shaoming Huang Krishna K. Gali Reddy V. B. Lachagari Navajeet Chakravartty Rosalind A. Bueckert Bunyamin Tar’an Thomas D. Warkentin Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
description	Field pea (Pisum sativum L.), a cool-season legume crop, is known for poor heat tolerance. Our previous work identified PR11-2 and PR11-90 as heat tolerant and susceptible lines in a recombinant inbred population. CDC Amarillo, a Canadian elite pea variety, was considered as another heat tolerant variety based on its similar field performance as PR11-2. This study aimed to characterize the differential transcription. Plants of these three varieties were stressed for 3 h at 38°C prior to self-pollination, and RNAs from heat stressed anthers and stipules on the same flowering node were extracted and sequenced via the Illumina NovaSeq platform for the characterization of heat responsive genes. In silico results were further validated by qPCR assay. Differentially expressed genes (DEGs) were identified at log2 \|fold change (FC)\| ≥ 2 between high temperature and control temperature, the three varieties shared 588 DEGs which were up-regulated and 220 genes which were down-regulated in anthers when subjected to heat treatment. In stipules, 879 DEGs (463/416 upregulation/downregulation) were consistent among varieties. The above heat-induced genes of the two plant organs were related to several biological processes i.e., response to heat, protein folding and DNA templated transcription. Ten gene ontology (GO) terms were over-represented in the consistently down-regulated DEGs of the two organs, and these terms were mainly related to cell wall macromolecule metabolism, lipid transport, lipid localization, and lipid metabolic processes. GO enrichment analysis on distinct DEGs of individual pea varieties suggested that heat affected biological processes were dynamic, and variety distinct responses provide insight into molecular mechanisms of heat-tolerance response. Several biological processes, e.g., cellular response to DNA damage stimulus in stipule, electron transport chain in anther that were only observed in heat induced PR11-2 and CDC Amarillo, and their relevance to field pea heat tolerance is worth further validation.
format	article
author	Shaoming Huang Krishna K. Gali Reddy V. B. Lachagari Navajeet Chakravartty Rosalind A. Bueckert Bunyamin Tar’an Thomas D. Warkentin
author_facet	Shaoming Huang Krishna K. Gali Reddy V. B. Lachagari Navajeet Chakravartty Rosalind A. Bueckert Bunyamin Tar’an Thomas D. Warkentin
author_sort	Shaoming Huang
title	Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
title_short	Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
title_full	Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
title_fullStr	Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
title_full_unstemmed	Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
title_sort	identification of heat responsive genes in pea stipules and anthers through transcriptional profiling
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/839db5162082416cb237412cdac4f05c
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Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling

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