A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice

Joshua Crawford, Sufang Liu, Feng Tao Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, TX, 75246, USACorrespondence: Feng TaoDepartment of Biomedical Sciences, Texas A&M University College of Dentistry, 3302 Gaston Ave., Dallas, TX, 75246, USATel +1-214-8...

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Autores principales:	Crawford J, Liu S, Tao F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	optogenetic stimulation multi-channel recording real-time place preference Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/83af863a19ee4f4096c25afd62423837
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spelling	oai:doaj.org-article:83af863a19ee4f4096c25afd624238372021-11-09T18:40:35ZA Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice1178-7090https://doaj.org/article/83af863a19ee4f4096c25afd624238372021-11-01T00:00:00Zhttps://www.dovepress.com/a-multidisciplinary-approach-to-simultaneously-monitoring-real-time-ne-peer-reviewed-fulltext-article-JPRhttps://doaj.org/toc/1178-7090Joshua Crawford, Sufang Liu, Feng Tao Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, TX, 75246, USACorrespondence: Feng TaoDepartment of Biomedical Sciences, Texas A&M University College of Dentistry, 3302 Gaston Ave., Dallas, TX, 75246, USATel +1-214-828-8272Fax +1-214-874-4538Email ftao81@tamu.eduBackground: Highlighted by the current opioid epidemic, identifying novel therapies to treat chronic trigeminal neuropathic pain is a critical need. To develop these treatments, it is necessary to have viable targets in the brain to act on. Historically, neural tracing studies have been extremely useful in determining connections between brain areas but do not provide information about the functionality of these connections. Combining optogenetics and behavioral observation allows researchers to determine whether a particular brain area is involved in the regulation of such behavior. The addition of multi-channel electrophysiological recording provides information on real-time neuronal activity in the specific neuronal pathway.Methods: Male C57/BL/6J mice (8-week-old) underwent either chronic constriction injury of infraorbital nerve (CCI-ION) or a sham surgery and were injected with either channelrhodopsin (ChR2) or a control virus in the hypothalamic A11 nucleus. Two weeks after CCI-ION, they were tested in real-time place preference (RTPP), while neuronal activity in the spinal trigeminal nucleus caudalis (Sp5C) was recorded.Results: Optogenetic excitation of the A11 neurons results in more time spent in the stimulation chamber during RTPP testing. Additionally, stimulation of the A11 results in a greater number of neuronal activity increase in the Sp5C in animals with the injection of AAV carrying ChR2 compared to animals injected with a control virus or that underwent a sham surgery.Conclusion: In vivo multi-channel electrophysiological recording, optogenetic stimulation, and behavioral observation can be combined in a mouse model of chronic trigeminal neuropathic pain to validate brain areas involved in the modulation of such pain.Keywords: optogenetic stimulation, multi-channel recording, real-time place preferenceCrawford JLiu STao FDove Medical Pressarticleoptogenetic stimulationmulti-channel recordingreal-time place preferenceMedicine (General)R5-920ENJournal of Pain Research, Vol Volume 14, Pp 3503-3509 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	optogenetic stimulation multi-channel recording real-time place preference Medicine (General) R5-920
spellingShingle	optogenetic stimulation multi-channel recording real-time place preference Medicine (General) R5-920 Crawford J Liu S Tao F A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice
description	Joshua Crawford, Sufang Liu, Feng Tao Department of Biomedical Sciences, Texas A&M University College of Dentistry, Dallas, TX, 75246, USACorrespondence: Feng TaoDepartment of Biomedical Sciences, Texas A&M University College of Dentistry, 3302 Gaston Ave., Dallas, TX, 75246, USATel +1-214-828-8272Fax +1-214-874-4538Email ftao81@tamu.eduBackground: Highlighted by the current opioid epidemic, identifying novel therapies to treat chronic trigeminal neuropathic pain is a critical need. To develop these treatments, it is necessary to have viable targets in the brain to act on. Historically, neural tracing studies have been extremely useful in determining connections between brain areas but do not provide information about the functionality of these connections. Combining optogenetics and behavioral observation allows researchers to determine whether a particular brain area is involved in the regulation of such behavior. The addition of multi-channel electrophysiological recording provides information on real-time neuronal activity in the specific neuronal pathway.Methods: Male C57/BL/6J mice (8-week-old) underwent either chronic constriction injury of infraorbital nerve (CCI-ION) or a sham surgery and were injected with either channelrhodopsin (ChR2) or a control virus in the hypothalamic A11 nucleus. Two weeks after CCI-ION, they were tested in real-time place preference (RTPP), while neuronal activity in the spinal trigeminal nucleus caudalis (Sp5C) was recorded.Results: Optogenetic excitation of the A11 neurons results in more time spent in the stimulation chamber during RTPP testing. Additionally, stimulation of the A11 results in a greater number of neuronal activity increase in the Sp5C in animals with the injection of AAV carrying ChR2 compared to animals injected with a control virus or that underwent a sham surgery.Conclusion: In vivo multi-channel electrophysiological recording, optogenetic stimulation, and behavioral observation can be combined in a mouse model of chronic trigeminal neuropathic pain to validate brain areas involved in the modulation of such pain.Keywords: optogenetic stimulation, multi-channel recording, real-time place preference
format	article
author	Crawford J Liu S Tao F
author_facet	Crawford J Liu S Tao F
author_sort	Crawford J
title	A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice
title_short	A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice
title_full	A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice
title_fullStr	A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice
title_full_unstemmed	A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice
title_sort	multidisciplinary approach to simultaneously monitoring real-time neuronal activity and pain behaviors during optogenetic stimulation of brain neurons in freely moving mice
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/83af863a19ee4f4096c25afd62423837
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A Multidisciplinary Approach to Simultaneously Monitoring Real-Time Neuronal Activity and Pain Behaviors During Optogenetic Stimulation of Brain Neurons in Freely Moving Mice

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